Pata virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; unclassified Orbivirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9ZXS9|H9ZXS9_9REOV Core protein VP7 OS=Pata virus OX=1170506 PE=3 SV=1
MM1 pKa = 6.71EE2 pKa = 4.21QKK4 pKa = 10.01PRR6 pKa = 11.84RR7 pKa = 11.84FTKK10 pKa = 9.12TICVYY15 pKa = 10.44DD16 pKa = 3.67PSGRR20 pKa = 11.84TICGKK25 pKa = 8.22VAKK28 pKa = 9.72EE29 pKa = 3.83KK30 pKa = 10.17RR31 pKa = 11.84DD32 pKa = 3.39PYY34 pKa = 11.25AIIKK38 pKa = 9.48LGRR41 pKa = 11.84VFSLKK46 pKa = 10.61SADD49 pKa = 3.57GPQPKK54 pKa = 10.06SYY56 pKa = 10.55VLEE59 pKa = 3.93ISRR62 pKa = 11.84PGAYY66 pKa = 9.56RR67 pKa = 11.84IQDD70 pKa = 3.68GQDD73 pKa = 3.4TISLMIEE80 pKa = 3.92EE81 pKa = 4.77NGVEE85 pKa = 4.23GTTEE89 pKa = 3.79RR90 pKa = 11.84WEE92 pKa = 3.85EE93 pKa = 3.97WKK95 pKa = 10.94FEE97 pKa = 4.36TLNPMPMATMIRR109 pKa = 11.84HH110 pKa = 5.84NGQMVDD116 pKa = 5.42AEE118 pKa = 4.39LKK120 pKa = 7.92YY121 pKa = 10.58CKK123 pKa = 10.0GMGMIEE129 pKa = 4.91PYY131 pKa = 9.5TKK133 pKa = 10.72NDD135 pKa = 3.4FDD137 pKa = 4.68RR138 pKa = 11.84KK139 pKa = 10.01EE140 pKa = 3.97MPALPGVEE148 pKa = 3.72QSKK151 pKa = 10.28YY152 pKa = 6.9EE153 pKa = 4.0VRR155 pKa = 11.84EE156 pKa = 3.68LRR158 pKa = 11.84QKK160 pKa = 10.24IRR162 pKa = 11.84EE163 pKa = 4.02EE164 pKa = 4.08RR165 pKa = 11.84EE166 pKa = 3.91AQAKK170 pKa = 9.79GLLPQPRR177 pKa = 11.84DD178 pKa = 3.25MRR180 pKa = 11.84VRR182 pKa = 11.84EE183 pKa = 4.03EE184 pKa = 3.93EE185 pKa = 3.87QMQRR189 pKa = 11.84AQEE192 pKa = 4.08VVRR195 pKa = 11.84QEE197 pKa = 3.65KK198 pKa = 9.38TSYY201 pKa = 10.16KK202 pKa = 9.91WEE204 pKa = 4.44DD205 pKa = 4.87DD206 pKa = 3.34IDD208 pKa = 4.07FDD210 pKa = 5.02QPKK213 pKa = 8.88DD214 pKa = 3.49TQVRR218 pKa = 11.84KK219 pKa = 10.58DD220 pKa = 3.42FDD222 pKa = 3.98EE223 pKa = 4.12EE224 pKa = 3.91RR225 pKa = 11.84HH226 pKa = 5.36RR227 pKa = 11.84QEE229 pKa = 4.1QRR231 pKa = 11.84AQRR234 pKa = 11.84RR235 pKa = 11.84NEE237 pKa = 3.62QNEE240 pKa = 3.88RR241 pKa = 11.84DD242 pKa = 3.9EE243 pKa = 4.16EE244 pKa = 4.19LHH246 pKa = 6.62RR247 pKa = 11.84KK248 pKa = 9.66LEE250 pKa = 4.14AFEE253 pKa = 4.42QNILNKK259 pKa = 9.86AEE261 pKa = 4.02KK262 pKa = 9.42MEE264 pKa = 4.26QIVEE268 pKa = 4.32EE269 pKa = 4.11VSEE272 pKa = 4.08EE273 pKa = 4.23SEE275 pKa = 4.06EE276 pKa = 4.38EE277 pKa = 4.16EE278 pKa = 4.39VVEE281 pKa = 4.75SYY283 pKa = 8.59ITAEE287 pKa = 4.12YY288 pKa = 9.21IEE290 pKa = 5.05RR291 pKa = 11.84MSKK294 pKa = 7.45QQKK297 pKa = 8.15TKK299 pKa = 10.66DD300 pKa = 3.66EE301 pKa = 4.49RR302 pKa = 11.84FMSYY306 pKa = 10.73AQMMPQAQGDD316 pKa = 3.87FDD318 pKa = 5.94RR319 pKa = 11.84ILVTKK324 pKa = 9.49KK325 pKa = 10.66VKK327 pKa = 9.24WDD329 pKa = 3.61NVPLFIVNEE338 pKa = 4.01SRR340 pKa = 11.84KK341 pKa = 10.12AYY343 pKa = 8.57EE344 pKa = 3.66LQAIGEE350 pKa = 4.56CVRR353 pKa = 11.84VVFAVKK359 pKa = 8.85GTNLLILPAGVV370 pKa = 3.13

Molecular weight: 43.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9ZXT1|H9ZXT1_9REOV Non-structural protein NS3 OS=Pata virus OX=1170506 PE=3 SV=1
MM1 pKa = 7.81LSRR4 pKa = 11.84LIAKK8 pKa = 9.99YY9 pKa = 9.9EE10 pKa = 3.93EE11 pKa = 4.56EE12 pKa = 3.8KK13 pKa = 10.65TEE15 pKa = 3.8IYY17 pKa = 10.82EE18 pKa = 4.1MNKK21 pKa = 7.9TQNEE25 pKa = 4.13TLEE28 pKa = 4.07LVRR31 pKa = 11.84YY32 pKa = 9.32DD33 pKa = 3.9EE34 pKa = 4.62SAARR38 pKa = 11.84PPSYY42 pKa = 11.04APTAPVQIPGMPSVSLDD59 pKa = 3.43ILDD62 pKa = 4.08KK63 pKa = 11.5AMSNTTGTTNAQKK76 pKa = 10.58EE77 pKa = 4.26EE78 pKa = 3.94KK79 pKa = 10.06AAYY82 pKa = 9.79ASYY85 pKa = 11.6AEE87 pKa = 4.21AFRR90 pKa = 11.84DD91 pKa = 3.68NLRR94 pKa = 11.84VRR96 pKa = 11.84QVKK99 pKa = 9.93RR100 pKa = 11.84RR101 pKa = 11.84VNEE104 pKa = 3.86QILPRR109 pKa = 11.84LKK111 pKa = 10.08TEE113 pKa = 3.83LRR115 pKa = 11.84GLKK118 pKa = 8.17TRR120 pKa = 11.84KK121 pKa = 8.24TIIHH125 pKa = 5.87ITLLVAATAALLTSVSTLTGDD146 pKa = 4.38LSITIPAIGQANSTKK161 pKa = 10.01IEE163 pKa = 3.77MPAWFKK169 pKa = 11.22GFSTVFGIINLASTTVMLISARR191 pKa = 11.84QEE193 pKa = 3.88RR194 pKa = 11.84ALDD197 pKa = 3.22AHH199 pKa = 6.53IAMLRR204 pKa = 11.84KK205 pKa = 9.78EE206 pKa = 4.12IMKK209 pKa = 8.95KK210 pKa = 8.2TSYY213 pKa = 11.14NEE215 pKa = 3.9AVHH218 pKa = 6.32MSMTEE223 pKa = 3.86LSEE226 pKa = 4.25VPLDD230 pKa = 3.71GFEE233 pKa = 4.66IPMTT237 pKa = 4.11

Molecular weight: 26.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6181

237

1303

618.1

70.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.973 ± 0.602

0.955 ± 0.232

5.468 ± 0.309

8.041 ± 0.65

4.028 ± 0.346

5.452 ± 0.309

2.039 ± 0.197

6.973 ± 0.208

5.646 ± 0.619

9.157 ± 0.44

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.333 ± 0.326

4.239 ± 0.331

3.964 ± 0.358

4.158 ± 0.392

7.199 ± 0.254

5.485 ± 0.367

5.274 ± 0.401

6.569 ± 0.324

1.23 ± 0.17

3.818 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski