Amino acid dipepetide frequency for Tsukamurella phage TPA4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.347AlaAla: 21.347 ± 2.276
1.025AlaCys: 1.025 ± 0.281
7.742AlaAsp: 7.742 ± 0.651
7.856AlaGlu: 7.856 ± 0.608
2.619AlaPhe: 2.619 ± 0.341
11.157AlaGly: 11.157 ± 0.856
2.049AlaHis: 2.049 ± 0.35
4.44AlaIle: 4.44 ± 0.561
3.188AlaLys: 3.188 ± 0.649
10.417AlaLeu: 10.417 ± 0.869
3.472AlaMet: 3.472 ± 0.385
2.732AlaAsn: 2.732 ± 0.407
7.4AlaPro: 7.4 ± 0.833
4.782AlaGln: 4.782 ± 0.57
9.165AlaArg: 9.165 ± 0.9
5.579AlaSer: 5.579 ± 0.766
7.628AlaThr: 7.628 ± 0.801
9.677AlaVal: 9.677 ± 0.937
2.619AlaTrp: 2.619 ± 0.412
2.22AlaTyr: 2.22 ± 0.395
0.0AlaXaa: 0.0 ± 0.0
Cys
1.138CysAla: 1.138 ± 0.318
0.114CysCys: 0.114 ± 0.077
0.74CysAsp: 0.74 ± 0.247
0.342CysGlu: 0.342 ± 0.12
0.171CysPhe: 0.171 ± 0.101
1.48CysGly: 1.48 ± 0.307
0.228CysHis: 0.228 ± 0.112
0.228CysIle: 0.228 ± 0.107
0.342CysLys: 0.342 ± 0.148
0.285CysLeu: 0.285 ± 0.134
0.285CysMet: 0.285 ± 0.119
0.114CysAsn: 0.114 ± 0.071
1.025CysPro: 1.025 ± 0.27
0.114CysGln: 0.114 ± 0.074
0.854CysArg: 0.854 ± 0.265
0.398CysSer: 0.398 ± 0.119
0.797CysThr: 0.797 ± 0.232
0.569CysVal: 0.569 ± 0.177
0.114CysTrp: 0.114 ± 0.084
0.114CysTyr: 0.114 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
8.026AspAla: 8.026 ± 0.565
0.626AspCys: 0.626 ± 0.187
4.896AspAsp: 4.896 ± 0.685
3.985AspGlu: 3.985 ± 0.549
1.935AspPhe: 1.935 ± 0.305
6.717AspGly: 6.717 ± 0.803
1.138AspHis: 1.138 ± 0.378
1.879AspIle: 1.879 ± 0.322
1.423AspLys: 1.423 ± 0.241
6.433AspLeu: 6.433 ± 0.574
1.025AspMet: 1.025 ± 0.257
1.195AspAsn: 1.195 ± 0.243
5.636AspPro: 5.636 ± 0.485
2.22AspGln: 2.22 ± 0.339
5.237AspArg: 5.237 ± 0.551
2.448AspSer: 2.448 ± 0.427
4.212AspThr: 4.212 ± 0.428
3.985AspVal: 3.985 ± 0.539
1.423AspTrp: 1.423 ± 0.285
2.106AspTyr: 2.106 ± 0.384
0.0AspXaa: 0.0 ± 0.0
Glu
6.603GluAla: 6.603 ± 0.7
0.455GluCys: 0.455 ± 0.172
2.903GluAsp: 2.903 ± 0.403
2.562GluGlu: 2.562 ± 0.383
1.537GluPhe: 1.537 ± 0.34
3.302GluGly: 3.302 ± 0.499
2.106GluHis: 2.106 ± 0.356
2.505GluIle: 2.505 ± 0.322
1.879GluLys: 1.879 ± 0.375
5.123GluLeu: 5.123 ± 0.608
0.683GluMet: 0.683 ± 0.151
0.968GluAsn: 0.968 ± 0.211
3.757GluPro: 3.757 ± 0.662
2.903GluGln: 2.903 ± 0.431
4.554GluArg: 4.554 ± 0.595
2.732GluSer: 2.732 ± 0.318
3.074GluThr: 3.074 ± 0.376
4.44GluVal: 4.44 ± 0.588
1.252GluTrp: 1.252 ± 0.272
1.252GluTyr: 1.252 ± 0.25
0.0GluXaa: 0.0 ± 0.0
Phe
2.163PheAla: 2.163 ± 0.363
0.171PheCys: 0.171 ± 0.101
1.822PheAsp: 1.822 ± 0.332
1.594PheGlu: 1.594 ± 0.275
0.683PhePhe: 0.683 ± 0.198
3.302PheGly: 3.302 ± 0.445
0.398PheHis: 0.398 ± 0.13
1.195PheIle: 1.195 ± 0.273
0.911PheLys: 0.911 ± 0.226
1.48PheLeu: 1.48 ± 0.277
0.228PheMet: 0.228 ± 0.098
0.968PheAsn: 0.968 ± 0.218
1.594PhePro: 1.594 ± 0.368
0.911PheGln: 0.911 ± 0.196
1.935PheArg: 1.935 ± 0.252
0.968PheSer: 0.968 ± 0.214
2.334PheThr: 2.334 ± 0.347
1.366PheVal: 1.366 ± 0.207
0.285PheTrp: 0.285 ± 0.106
0.398PheTyr: 0.398 ± 0.143
0.0PheXaa: 0.0 ± 0.0
Gly
10.19GlyAla: 10.19 ± 1.027
1.195GlyCys: 1.195 ± 0.286
6.091GlyAsp: 6.091 ± 0.683
5.18GlyGlu: 5.18 ± 0.863
2.106GlyPhe: 2.106 ± 0.341
10.645GlyGly: 10.645 ± 1.516
2.049GlyHis: 2.049 ± 0.237
3.472GlyIle: 3.472 ± 0.346
3.359GlyLys: 3.359 ± 0.43
6.66GlyLeu: 6.66 ± 0.676
2.163GlyMet: 2.163 ± 0.362
2.619GlyAsn: 2.619 ± 0.408
4.497GlyPro: 4.497 ± 0.548
3.529GlyGln: 3.529 ± 0.434
6.831GlyArg: 6.831 ± 0.621
6.091GlySer: 6.091 ± 1.081
7.286GlyThr: 7.286 ± 0.819
6.717GlyVal: 6.717 ± 0.751
2.106GlyTrp: 2.106 ± 0.31
1.992GlyTyr: 1.992 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
2.163HisAla: 2.163 ± 0.323
0.342HisCys: 0.342 ± 0.147
2.163HisAsp: 2.163 ± 0.396
0.797HisGlu: 0.797 ± 0.211
0.342HisPhe: 0.342 ± 0.129
2.049HisGly: 2.049 ± 0.349
1.025HisHis: 1.025 ± 0.267
0.74HisIle: 0.74 ± 0.16
0.455HisLys: 0.455 ± 0.128
1.822HisLeu: 1.822 ± 0.304
0.342HisMet: 0.342 ± 0.153
0.342HisAsn: 0.342 ± 0.161
1.935HisPro: 1.935 ± 0.42
0.968HisGln: 0.968 ± 0.269
1.423HisArg: 1.423 ± 0.277
0.569HisSer: 0.569 ± 0.174
1.423HisThr: 1.423 ± 0.256
1.48HisVal: 1.48 ± 0.335
0.342HisTrp: 0.342 ± 0.218
0.455HisTyr: 0.455 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
5.636IleAla: 5.636 ± 0.605
0.342IleCys: 0.342 ± 0.143
3.7IleAsp: 3.7 ± 0.42
2.163IleGlu: 2.163 ± 0.332
0.455IlePhe: 0.455 ± 0.178
3.7IleGly: 3.7 ± 0.395
0.626IleHis: 0.626 ± 0.245
1.423IleIle: 1.423 ± 0.276
0.626IleLys: 0.626 ± 0.171
3.188IleLeu: 3.188 ± 0.442
0.512IleMet: 0.512 ± 0.157
1.195IleAsn: 1.195 ± 0.293
2.22IlePro: 2.22 ± 0.336
1.082IleGln: 1.082 ± 0.216
3.586IleArg: 3.586 ± 0.424
1.195IleSer: 1.195 ± 0.215
3.928IleThr: 3.928 ± 0.469
2.846IleVal: 2.846 ± 0.348
0.569IleTrp: 0.569 ± 0.151
0.683IleTyr: 0.683 ± 0.16
0.0IleXaa: 0.0 ± 0.0
Lys
3.359LysAla: 3.359 ± 0.573
0.114LysCys: 0.114 ± 0.081
1.423LysAsp: 1.423 ± 0.299
1.48LysGlu: 1.48 ± 0.281
0.74LysPhe: 0.74 ± 0.213
1.708LysGly: 1.708 ± 0.349
0.626LysHis: 0.626 ± 0.228
1.025LysIle: 1.025 ± 0.239
1.423LysLys: 1.423 ± 0.377
2.619LysLeu: 2.619 ± 0.304
0.626LysMet: 0.626 ± 0.172
0.797LysAsn: 0.797 ± 0.236
1.594LysPro: 1.594 ± 0.247
0.569LysGln: 0.569 ± 0.143
2.22LysArg: 2.22 ± 0.391
1.879LysSer: 1.879 ± 0.377
2.846LysThr: 2.846 ± 0.541
2.448LysVal: 2.448 ± 0.371
0.455LysTrp: 0.455 ± 0.147
0.569LysTyr: 0.569 ± 0.155
0.0LysXaa: 0.0 ± 0.0
Leu
12.068LeuAla: 12.068 ± 0.765
0.968LeuCys: 0.968 ± 0.266
6.091LeuAsp: 6.091 ± 0.595
3.757LeuGlu: 3.757 ± 0.377
1.423LeuPhe: 1.423 ± 0.314
8.14LeuGly: 8.14 ± 0.499
1.594LeuHis: 1.594 ± 0.281
3.131LeuIle: 3.131 ± 0.477
1.594LeuLys: 1.594 ± 0.421
6.262LeuLeu: 6.262 ± 0.728
1.935LeuMet: 1.935 ± 0.385
2.96LeuAsn: 2.96 ± 0.496
4.896LeuPro: 4.896 ± 0.673
1.708LeuGln: 1.708 ± 0.352
5.806LeuArg: 5.806 ± 0.585
3.245LeuSer: 3.245 ± 0.395
6.376LeuThr: 6.376 ± 0.579
6.034LeuVal: 6.034 ± 0.696
1.138LeuTrp: 1.138 ± 0.308
1.309LeuTyr: 1.309 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
1.708MetAla: 1.708 ± 0.293
0.342MetCys: 0.342 ± 0.113
0.854MetAsp: 0.854 ± 0.284
1.082MetGlu: 1.082 ± 0.222
0.626MetPhe: 0.626 ± 0.202
1.651MetGly: 1.651 ± 0.407
0.455MetHis: 0.455 ± 0.196
0.455MetIle: 0.455 ± 0.146
0.797MetLys: 0.797 ± 0.199
1.708MetLeu: 1.708 ± 0.313
0.228MetMet: 0.228 ± 0.099
0.683MetAsn: 0.683 ± 0.207
2.106MetPro: 2.106 ± 0.312
0.626MetGln: 0.626 ± 0.163
1.423MetArg: 1.423 ± 0.272
1.594MetSer: 1.594 ± 0.359
2.732MetThr: 2.732 ± 0.363
1.195MetVal: 1.195 ± 0.218
0.342MetTrp: 0.342 ± 0.115
0.342MetTyr: 0.342 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.472AsnAla: 3.472 ± 0.473
0.114AsnCys: 0.114 ± 0.076
1.651AsnAsp: 1.651 ± 0.348
1.594AsnGlu: 1.594 ± 0.274
0.455AsnPhe: 0.455 ± 0.153
2.619AsnGly: 2.619 ± 0.343
0.911AsnHis: 0.911 ± 0.304
0.683AsnIle: 0.683 ± 0.184
0.854AsnLys: 0.854 ± 0.24
2.106AsnLeu: 2.106 ± 0.361
0.398AsnMet: 0.398 ± 0.132
0.626AsnAsn: 0.626 ± 0.244
1.822AsnPro: 1.822 ± 0.309
1.138AsnGln: 1.138 ± 0.249
2.334AsnArg: 2.334 ± 0.407
1.252AsnSer: 1.252 ± 0.282
1.537AsnThr: 1.537 ± 0.338
1.992AsnVal: 1.992 ± 0.37
0.114AsnTrp: 0.114 ± 0.069
0.398AsnTyr: 0.398 ± 0.114
0.0AsnXaa: 0.0 ± 0.0
Pro
8.596ProAla: 8.596 ± 0.93
0.74ProCys: 0.74 ± 0.239
4.668ProAsp: 4.668 ± 0.703
3.302ProGlu: 3.302 ± 0.53
1.822ProPhe: 1.822 ± 0.361
7.343ProGly: 7.343 ± 0.652
1.025ProHis: 1.025 ± 0.227
2.675ProIle: 2.675 ± 0.456
1.879ProLys: 1.879 ± 0.324
3.017ProLeu: 3.017 ± 0.43
1.48ProMet: 1.48 ± 0.345
1.423ProAsn: 1.423 ± 0.247
3.814ProPro: 3.814 ± 0.884
1.879ProGln: 1.879 ± 0.343
3.415ProArg: 3.415 ± 0.581
3.529ProSer: 3.529 ± 0.571
4.839ProThr: 4.839 ± 0.59
4.952ProVal: 4.952 ± 0.689
1.309ProTrp: 1.309 ± 0.314
1.082ProTyr: 1.082 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
4.611GlnAla: 4.611 ± 0.537
0.114GlnCys: 0.114 ± 0.076
1.423GlnAsp: 1.423 ± 0.277
1.366GlnGlu: 1.366 ± 0.226
0.854GlnPhe: 0.854 ± 0.218
1.992GlnGly: 1.992 ± 0.316
1.195GlnHis: 1.195 ± 0.311
1.309GlnIle: 1.309 ± 0.236
1.366GlnLys: 1.366 ± 0.267
4.269GlnLeu: 4.269 ± 0.502
0.569GlnMet: 0.569 ± 0.162
0.74GlnAsn: 0.74 ± 0.241
1.992GlnPro: 1.992 ± 0.359
1.879GlnGln: 1.879 ± 0.367
2.732GlnArg: 2.732 ± 0.388
1.537GlnSer: 1.537 ± 0.381
1.765GlnThr: 1.765 ± 0.367
2.619GlnVal: 2.619 ± 0.357
1.025GlnTrp: 1.025 ± 0.271
0.455GlnTyr: 0.455 ± 0.144
0.0GlnXaa: 0.0 ± 0.0
Arg
9.734ArgAla: 9.734 ± 0.906
1.025ArgCys: 1.025 ± 0.263
5.18ArgAsp: 5.18 ± 0.545
5.066ArgGlu: 5.066 ± 0.63
2.448ArgPhe: 2.448 ± 0.374
5.522ArgGly: 5.522 ± 0.56
2.163ArgHis: 2.163 ± 0.332
3.643ArgIle: 3.643 ± 0.461
1.708ArgLys: 1.708 ± 0.37
5.977ArgLeu: 5.977 ± 0.493
2.391ArgMet: 2.391 ± 0.465
2.22ArgAsn: 2.22 ± 0.325
3.586ArgPro: 3.586 ± 0.487
2.675ArgGln: 2.675 ± 0.406
5.237ArgArg: 5.237 ± 0.64
3.415ArgSer: 3.415 ± 0.368
4.782ArgThr: 4.782 ± 0.659
4.383ArgVal: 4.383 ± 0.529
1.594ArgTrp: 1.594 ± 0.291
2.334ArgTyr: 2.334 ± 0.356
0.0ArgXaa: 0.0 ± 0.0
Ser
5.066SerAla: 5.066 ± 0.851
0.512SerCys: 0.512 ± 0.199
3.302SerAsp: 3.302 ± 0.331
2.619SerGlu: 2.619 ± 0.43
1.594SerPhe: 1.594 ± 0.256
6.489SerGly: 6.489 ± 0.822
0.683SerHis: 0.683 ± 0.18
2.391SerIle: 2.391 ± 0.311
1.992SerLys: 1.992 ± 0.502
3.359SerLeu: 3.359 ± 0.551
1.537SerMet: 1.537 ± 0.269
1.082SerAsn: 1.082 ± 0.281
2.96SerPro: 2.96 ± 0.455
1.594SerGln: 1.594 ± 0.297
3.131SerArg: 3.131 ± 0.465
3.415SerSer: 3.415 ± 0.645
2.846SerThr: 2.846 ± 0.364
3.017SerVal: 3.017 ± 0.443
1.025SerTrp: 1.025 ± 0.284
0.968SerTyr: 0.968 ± 0.235
0.0SerXaa: 0.0 ± 0.0
Thr
8.71ThrAla: 8.71 ± 0.883
0.398ThrCys: 0.398 ± 0.17
4.554ThrAsp: 4.554 ± 0.688
3.131ThrGlu: 3.131 ± 0.405
2.334ThrPhe: 2.334 ± 0.497
7.856ThrGly: 7.856 ± 0.775
0.911ThrHis: 0.911 ± 0.24
3.643ThrIle: 3.643 ± 0.404
1.708ThrLys: 1.708 ± 0.343
5.351ThrLeu: 5.351 ± 0.659
1.309ThrMet: 1.309 ± 0.264
2.106ThrAsn: 2.106 ± 0.37
4.782ThrPro: 4.782 ± 0.511
1.423ThrGln: 1.423 ± 0.27
5.066ThrArg: 5.066 ± 0.539
3.757ThrSer: 3.757 ± 0.483
5.18ThrThr: 5.18 ± 0.683
7.856ThrVal: 7.856 ± 0.757
1.025ThrTrp: 1.025 ± 0.205
1.366ThrTyr: 1.366 ± 0.316
0.0ThrXaa: 0.0 ± 0.0
Val
8.937ValAla: 8.937 ± 0.884
0.626ValCys: 0.626 ± 0.192
4.554ValAsp: 4.554 ± 0.442
4.952ValGlu: 4.952 ± 0.755
1.594ValPhe: 1.594 ± 0.265
5.408ValGly: 5.408 ± 0.689
1.082ValHis: 1.082 ± 0.223
3.017ValIle: 3.017 ± 0.387
2.106ValLys: 2.106 ± 0.303
7.002ValLeu: 7.002 ± 0.84
0.911ValMet: 0.911 ± 0.224
2.106ValAsn: 2.106 ± 0.374
4.269ValPro: 4.269 ± 0.585
2.22ValGln: 2.22 ± 0.366
6.888ValArg: 6.888 ± 0.733
3.928ValSer: 3.928 ± 0.459
5.18ValThr: 5.18 ± 0.545
5.351ValVal: 5.351 ± 0.826
1.252ValTrp: 1.252 ± 0.273
1.765ValTyr: 1.765 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
1.366TrpAla: 1.366 ± 0.321
0.171TrpCys: 0.171 ± 0.093
0.911TrpAsp: 0.911 ± 0.254
0.854TrpGlu: 0.854 ± 0.239
0.569TrpPhe: 0.569 ± 0.162
1.195TrpGly: 1.195 ± 0.297
0.683TrpHis: 0.683 ± 0.22
0.74TrpIle: 0.74 ± 0.169
0.512TrpLys: 0.512 ± 0.175
1.822TrpLeu: 1.822 ± 0.361
0.569TrpMet: 0.569 ± 0.18
0.74TrpAsn: 0.74 ± 0.203
1.423TrpPro: 1.423 ± 0.244
0.854TrpGln: 0.854 ± 0.247
1.48TrpArg: 1.48 ± 0.325
1.025TrpSer: 1.025 ± 0.26
2.163TrpThr: 2.163 ± 0.406
1.138TrpVal: 1.138 ± 0.231
0.285TrpTrp: 0.285 ± 0.126
0.228TrpTyr: 0.228 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.277TyrAla: 2.277 ± 0.352
0.057TyrCys: 0.057 ± 0.059
1.879TyrAsp: 1.879 ± 0.346
0.911TyrGlu: 0.911 ± 0.22
0.398TyrPhe: 0.398 ± 0.168
2.505TyrGly: 2.505 ± 0.386
0.228TyrHis: 0.228 ± 0.115
1.082TyrIle: 1.082 ± 0.248
0.398TyrLys: 0.398 ± 0.144
1.537TyrLeu: 1.537 ± 0.302
0.228TyrMet: 0.228 ± 0.099
0.455TyrAsn: 0.455 ± 0.157
1.48TyrPro: 1.48 ± 0.356
0.74TyrGln: 0.74 ± 0.21
1.765TyrArg: 1.765 ± 0.333
1.025TyrSer: 1.025 ± 0.257
1.537TyrThr: 1.537 ± 0.286
1.082TyrVal: 1.082 ± 0.219
0.455TyrTrp: 0.455 ± 0.13
0.342TyrTyr: 0.342 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (17568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski