Xylaria multiplex

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariaceae; Xylaria

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C8IYH7|A0A7C8IYH7_9PEZI Uncharacterized protein OS=Xylaria multiplex OX=323545 GN=GQX73_g1344 PE=4 SV=1
MM1 pKa = 7.26QLEE4 pKa = 4.85KK5 pKa = 10.6ILILLSVAALGAAEE19 pKa = 4.47NDD21 pKa = 3.54VEE23 pKa = 5.62ADD25 pKa = 4.1DD26 pKa = 5.15VPSACLPACQFTIDD40 pKa = 4.25LSARR44 pKa = 11.84CDD46 pKa = 3.33RR47 pKa = 11.84QTNSDD52 pKa = 3.85DD53 pKa = 3.46QYY55 pKa = 10.65TPCVCEE61 pKa = 4.24AQDD64 pKa = 3.16SRR66 pKa = 11.84QRR68 pKa = 11.84LTEE71 pKa = 4.03CASCVKK77 pKa = 10.63DD78 pKa = 4.06HH79 pKa = 6.55GRR81 pKa = 11.84SDD83 pKa = 3.85PDD85 pKa = 4.05DD86 pKa = 4.74NDD88 pKa = 3.5VADD91 pKa = 5.75LMDD94 pKa = 4.51DD95 pKa = 4.4CGWDD99 pKa = 3.63FNDD102 pKa = 4.67ADD104 pKa = 4.04VPYY107 pKa = 7.98PTSWSTTTAAIPSSTSAPASASTNPTGSTTTVVSQSSSQTLSQTPSQTSSSSSTTTVTGAPADD170 pKa = 3.91ATSSPDD176 pKa = 3.23SMAGALSAKK185 pKa = 9.07TVVGSLLAGLAISFFAMFF203 pKa = 4.77

Molecular weight: 20.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C8J1C8|A0A7C8J1C8_9PEZI DLH domain-containing protein OS=Xylaria multiplex OX=323545 GN=GQX73_g2059 PE=4 SV=1
MM1 pKa = 7.15QATPKK6 pKa = 10.42GATHH10 pKa = 7.34APQTGSFAIDD20 pKa = 3.07PTGYY24 pKa = 9.92NRR26 pKa = 11.84SAAGALPRR34 pKa = 11.84GSWRR38 pKa = 11.84ARR40 pKa = 11.84GPRR43 pKa = 11.84EE44 pKa = 4.07DD45 pKa = 3.22RR46 pKa = 11.84GSRR49 pKa = 11.84CRR51 pKa = 11.84RR52 pKa = 11.84GCHH55 pKa = 5.42GRR57 pKa = 11.84ASSQQEE63 pKa = 3.77SRR65 pKa = 11.84QLRR68 pKa = 11.84EE69 pKa = 3.95RR70 pKa = 11.84NRR72 pKa = 11.84CGPDD76 pKa = 3.08HH77 pKa = 6.66VPSGRR82 pKa = 11.84LKK84 pKa = 10.52VPGVLTISPLRR95 pKa = 11.84PGVMVAFSALIASSAIRR112 pKa = 11.84WLSAGTGVSLAGAGAVGWLYY132 pKa = 8.1QQKK135 pKa = 10.31VGSILYY141 pKa = 9.69AAVITRR147 pKa = 11.84PDD149 pKa = 3.11IAFGASRR156 pKa = 11.84LVRR159 pKa = 11.84FNHH162 pKa = 5.63NPSPEE167 pKa = 3.84HH168 pKa = 6.47HH169 pKa = 6.7KK170 pKa = 10.93AADD173 pKa = 3.86RR174 pKa = 11.84LLSYY178 pKa = 10.72TDD180 pKa = 3.41DD181 pKa = 3.55WLNKK185 pKa = 7.67ITRR188 pKa = 11.84ADD190 pKa = 3.58TEE192 pKa = 4.46GADD195 pKa = 3.33VTEE198 pKa = 4.42PFKK201 pKa = 11.03LTTDD205 pKa = 3.8LEE207 pKa = 4.52KK208 pKa = 11.13AHH210 pKa = 7.09DD211 pKa = 4.73LPTLPRR217 pKa = 11.84PLRR220 pKa = 11.84FGTVTVLKK228 pKa = 10.75LL229 pKa = 3.28

Molecular weight: 24.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10963

0

10963

5903969

66

6056

538.5

59.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.482 ± 0.019

1.178 ± 0.008

5.835 ± 0.015

6.117 ± 0.02

3.726 ± 0.013

6.847 ± 0.018

2.382 ± 0.01

5.219 ± 0.016

4.716 ± 0.018

8.96 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.009

3.846 ± 0.011

5.98 ± 0.023

3.921 ± 0.014

6.086 ± 0.021

8.167 ± 0.027

6.08 ± 0.016

6.089 ± 0.016

1.494 ± 0.008

2.838 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski