Lactococcus phage P335

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5SP23|B5SP23_9CAUD Putative ABC-transporter OS=Lactococcus phage P335 OX=201846 PE=4 SV=1
MM1 pKa = 6.9EE2 pKa = 5.22TIIKK6 pKa = 9.69KK7 pKa = 9.93VSEE10 pKa = 4.85LIPYY14 pKa = 9.52INNPRR19 pKa = 11.84NNDD22 pKa = 3.32EE23 pKa = 4.72AVDD26 pKa = 3.99AVASSIKK33 pKa = 10.62NFGFKK38 pKa = 10.69VPIVVDD44 pKa = 3.98SNNEE48 pKa = 3.83IINGHH53 pKa = 5.17TRR55 pKa = 11.84FKK57 pKa = 10.67AAKK60 pKa = 8.88KK61 pKa = 10.2LGLEE65 pKa = 4.13TVPDD69 pKa = 3.97IVADD73 pKa = 4.09DD74 pKa = 4.22LTPEE78 pKa = 4.0QIKK81 pKa = 10.64AFRR84 pKa = 11.84LADD87 pKa = 3.58NKK89 pKa = 10.4VGEE92 pKa = 4.18IATWDD97 pKa = 3.67EE98 pKa = 3.6NALAIEE104 pKa = 4.16LEE106 pKa = 4.19EE107 pKa = 4.87LKK109 pKa = 11.22NLDD112 pKa = 3.17FDD114 pKa = 3.54MSEE117 pKa = 4.46FEE119 pKa = 4.66FEE121 pKa = 3.72IDD123 pKa = 3.53EE124 pKa = 4.53PQAQEE129 pKa = 3.91KK130 pKa = 10.87DD131 pKa = 3.68EE132 pKa = 4.33NTDD135 pKa = 3.21SVLIDD140 pKa = 3.51EE141 pKa = 4.76KK142 pKa = 10.65MIIIIEE148 pKa = 4.07SDD150 pKa = 3.45NEE152 pKa = 4.47SKK154 pKa = 11.06LEE156 pKa = 3.95EE157 pKa = 4.26YY158 pKa = 10.49FEE160 pKa = 4.41KK161 pKa = 10.55LVEE164 pKa = 4.07EE165 pKa = 5.58GYY167 pKa = 9.85PCRR170 pKa = 11.84LSTLL174 pKa = 3.59

Molecular weight: 19.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5SP25|B5SP25_9CAUD Uncharacterized protein OS=Lactococcus phage P335 OX=201846 PE=4 SV=1
MM1 pKa = 7.72SSDD4 pKa = 3.34INNAAQCYY12 pKa = 8.66GVYY15 pKa = 10.58DD16 pKa = 3.81RR17 pKa = 11.84DD18 pKa = 3.8EE19 pKa = 3.99IVAFIGVIHH28 pKa = 6.74FPHH31 pKa = 6.85PKK33 pKa = 8.67NRR35 pKa = 11.84KK36 pKa = 6.79IKK38 pKa = 9.75KK39 pKa = 5.91VTRR42 pKa = 11.84LVVLPDD48 pKa = 3.41YY49 pKa = 10.95QGIGLGVAFLNTIAEE64 pKa = 4.21IYY66 pKa = 9.97KK67 pKa = 10.63DD68 pKa = 3.46YY69 pKa = 11.16DD70 pKa = 3.42FRR72 pKa = 11.84ITTSAKK78 pKa = 10.57NLIHH82 pKa = 7.19ALNKK86 pKa = 8.79HH87 pKa = 5.54KK88 pKa = 10.58NWRR91 pKa = 11.84LEE93 pKa = 3.67SFGRR97 pKa = 11.84HH98 pKa = 4.84KK99 pKa = 10.43FAASMKK105 pKa = 10.32KK106 pKa = 9.49EE107 pKa = 3.94MKK109 pKa = 10.06KK110 pKa = 10.29SFRR113 pKa = 11.84GHH115 pKa = 5.23VKK117 pKa = 8.15TASFIYY123 pKa = 10.25VSKK126 pKa = 11.18

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10213

31

918

204.3

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.345 ± 0.442

0.519 ± 0.095

6.188 ± 0.244

7.324 ± 0.634

4.191 ± 0.197

6.384 ± 0.528

1.204 ± 0.127

7.069 ± 0.289

9.106 ± 0.672

7.804 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.108

6.502 ± 0.291

2.732 ± 0.154

4.249 ± 0.275

3.584 ± 0.265

6.746 ± 0.336

6.129 ± 0.276

5.924 ± 0.303

1.4 ± 0.145

4.191 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski