Pedobacter sp. PACM 27299

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0NEP0|A0A0P0NEP0_9SPHI Anti-anti-sigma factor OS=Pedobacter sp. PACM 27299 OX=1727164 GN=AQ505_09200 PE=4 SV=1
MM1 pKa = 7.51NNLNHH6 pKa = 6.64LPSAAANANPKK17 pKa = 9.57EE18 pKa = 4.28EE19 pKa = 4.42NVNEE23 pKa = 4.13QPDD26 pKa = 3.74GHH28 pKa = 6.93MGMSPLIEE36 pKa = 4.24NFSLSNYY43 pKa = 9.74EE44 pKa = 5.57DD45 pKa = 5.58DD46 pKa = 5.98DD47 pKa = 5.71DD48 pKa = 6.85DD49 pKa = 6.25SGFSCPCCLPRR60 pKa = 6.09

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0NMX4|A0A0P0NMX4_9SPHI TonB-dependent receptor OS=Pedobacter sp. PACM 27299 OX=1727164 GN=AQ505_16620 PE=3 SV=1
MM1 pKa = 7.78GLRR4 pKa = 11.84KK5 pKa = 9.8FKK7 pKa = 10.46PVTPGTRR14 pKa = 11.84FRR16 pKa = 11.84VGASFSEE23 pKa = 4.18ITATKK28 pKa = 9.4PEE30 pKa = 3.92KK31 pKa = 10.78SLVVSSKK38 pKa = 10.94KK39 pKa = 10.5SGGRR43 pKa = 11.84NNTGKK48 pKa = 8.1MTMRR52 pKa = 11.84YY53 pKa = 8.57MGGGHH58 pKa = 6.3KK59 pKa = 10.01KK60 pKa = 9.96SYY62 pKa = 10.67RR63 pKa = 11.84LIDD66 pKa = 3.84FKK68 pKa = 10.93RR69 pKa = 11.84DD70 pKa = 3.21KK71 pKa = 11.05FNIPATVATVEE82 pKa = 3.94YY83 pKa = 10.46DD84 pKa = 3.37PNRR87 pKa = 11.84TARR90 pKa = 11.84IALLHH95 pKa = 5.81YY96 pKa = 10.78ADD98 pKa = 3.89GEE100 pKa = 4.06KK101 pKa = 10.2RR102 pKa = 11.84YY103 pKa = 10.16IIAPEE108 pKa = 4.2GLQVGQTVVSGDD120 pKa = 3.44TASPEE125 pKa = 3.94VGNALTLSRR134 pKa = 11.84IPLGSIVHH142 pKa = 6.53NIEE145 pKa = 3.71IQPGRR150 pKa = 11.84GAQMARR156 pKa = 11.84SAGAYY161 pKa = 8.57AQLAARR167 pKa = 11.84DD168 pKa = 3.89GKK170 pKa = 10.52YY171 pKa = 9.12ATVKK175 pKa = 9.72MPSGEE180 pKa = 3.85VRR182 pKa = 11.84AILVTCLATIGAVSNSDD199 pKa = 3.27HH200 pKa = 6.69ANEE203 pKa = 4.23VLGKK207 pKa = 10.21AGRR210 pKa = 11.84KK211 pKa = 7.93RR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.2GRR238 pKa = 11.84SSGGQPRR245 pKa = 11.84SRR247 pKa = 11.84NGLYY251 pKa = 10.68SKK253 pKa = 10.76GFKK256 pKa = 9.23TRR258 pKa = 11.84SLKK261 pKa = 10.55KK262 pKa = 8.95YY263 pKa = 7.96SNRR266 pKa = 11.84FIIEE270 pKa = 3.89KK271 pKa = 10.02RR272 pKa = 11.84KK273 pKa = 9.88KK274 pKa = 9.89

Molecular weight: 29.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4966

0

4966

1786279

36

6578

359.7

40.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.541 ± 0.037

0.718 ± 0.011

5.177 ± 0.022

5.792 ± 0.036

4.931 ± 0.026

6.86 ± 0.038

1.779 ± 0.019

7.184 ± 0.031

7.243 ± 0.04

9.835 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.018

5.635 ± 0.035

3.814 ± 0.017

3.778 ± 0.021

3.709 ± 0.023

6.559 ± 0.03

5.668 ± 0.056

6.173 ± 0.03

1.145 ± 0.013

4.094 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski