Rhodovulum imhoffii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5BNX2|A0A2T5BNX2_9RHOB Uncharacterized protein (Fragment) OS=Rhodovulum imhoffii OX=365340 GN=C8N32_1241 PE=4 SV=1
MM1 pKa = 7.18TQPQEE6 pKa = 4.11QLEE9 pKa = 4.57GTPLIQPSTTDD20 pKa = 2.96HH21 pKa = 7.04PLYY24 pKa = 10.66DD25 pKa = 3.96AVVEE29 pKa = 4.34ACRR32 pKa = 11.84SVFDD36 pKa = 4.4PEE38 pKa = 4.25IPVNIYY44 pKa = 10.8DD45 pKa = 4.42LGLIYY50 pKa = 10.21TIGISPEE57 pKa = 3.66NAVRR61 pKa = 11.84IIMTLTAPGCPVAGDD76 pKa = 3.6MPGWIVDD83 pKa = 4.88AISPVPGIKK92 pKa = 10.08EE93 pKa = 3.74VDD95 pKa = 3.83VEE97 pKa = 4.47ITWDD101 pKa = 4.11PPWGMDD107 pKa = 3.35MMSDD111 pKa = 3.68EE112 pKa = 4.89ARR114 pKa = 11.84LEE116 pKa = 4.14LGFMM120 pKa = 4.54

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5BNH8|A0A2T5BNH8_9RHOB Putative tricarboxylic transport membrane protein OS=Rhodovulum imhoffii OX=365340 GN=C8N32_1323 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2892

0

2892

883240

26

1836

305.4

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.532 ± 0.063

0.961 ± 0.016

5.668 ± 0.033

5.87 ± 0.041

3.686 ± 0.031

8.969 ± 0.047

2.083 ± 0.023

4.864 ± 0.03

2.882 ± 0.035

10.327 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.025

2.404 ± 0.022

5.372 ± 0.034

3.02 ± 0.025

7.559 ± 0.048

4.788 ± 0.029

5.424 ± 0.039

7.371 ± 0.041

1.374 ± 0.022

2.167 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski