Rubinisphaera brasiliensis (strain ATCC 49424 / DSM 5305 / JCM 21570 / NBRC 103401 / IFAM 1448) (Planctomyces brasiliensis)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Rubinisphaera; Rubinisphaera brasiliensis

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0SK84|F0SK84_RUBBR Uncharacterized protein OS=Rubinisphaera brasiliensis (strain ATCC 49424 / DSM 5305 / JCM 21570 / NBRC 103401 / IFAM 1448) OX=756272 GN=Plabr_3244 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 5.41APIDD6 pKa = 4.05PSSSLDD12 pKa = 3.28AVYY15 pKa = 10.94EE16 pKa = 4.04NGGLMFRR23 pKa = 11.84YY24 pKa = 8.96PGYY27 pKa = 9.21WQMEE31 pKa = 4.34EE32 pKa = 4.07FTSEE36 pKa = 4.23EE37 pKa = 4.01EE38 pKa = 3.97HH39 pKa = 6.84SATILTDD46 pKa = 4.04NSAFWMVSLIRR57 pKa = 11.84DD58 pKa = 3.66VGDD61 pKa = 3.06VDD63 pKa = 5.14AVLDD67 pKa = 3.72SALAAFEE74 pKa = 5.42DD75 pKa = 4.82EE76 pKa = 5.45YY77 pKa = 11.35EE78 pKa = 4.53DD79 pKa = 4.06LDD81 pKa = 3.64IYY83 pKa = 11.18EE84 pKa = 4.79RR85 pKa = 11.84VPDD88 pKa = 4.83KK89 pKa = 11.16PPGWTSQEE97 pKa = 5.09LEE99 pKa = 4.04FQYY102 pKa = 11.19QDD104 pKa = 3.32LVSSVVLQSIAGSHH118 pKa = 6.01GALLVLYY125 pKa = 9.67QGHH128 pKa = 6.98DD129 pKa = 3.37RR130 pKa = 11.84DD131 pKa = 4.29LEE133 pKa = 4.03EE134 pKa = 4.06HH135 pKa = 6.04QEE137 pKa = 3.9IFEE140 pKa = 4.16QMTASVLLQVV150 pKa = 4.29

Molecular weight: 16.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0SHS3|F0SHS3_RUBBR Uncharacterized protein OS=Rubinisphaera brasiliensis (strain ATCC 49424 / DSM 5305 / JCM 21570 / NBRC 103401 / IFAM 1448) OX=756272 GN=Plabr_1945 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 8.26TQRR6 pKa = 11.84KK7 pKa = 8.43LKK9 pKa = 9.88KK10 pKa = 9.26ANHH13 pKa = 6.11GKK15 pKa = 10.1RR16 pKa = 11.84PASAKK21 pKa = 9.9ARR23 pKa = 11.84RR24 pKa = 11.84AKK26 pKa = 10.21RR27 pKa = 11.84KK28 pKa = 9.13HH29 pKa = 5.85LRR31 pKa = 11.84FSS33 pKa = 3.37

Molecular weight: 3.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4710

0

4710

1698526

30

4724

360.6

39.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.325 ± 0.039

1.214 ± 0.015

5.89 ± 0.028

6.894 ± 0.035

3.849 ± 0.023

7.399 ± 0.038

2.246 ± 0.018

4.873 ± 0.023

3.759 ± 0.034

10.056 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.194 ± 0.015

3.429 ± 0.027

5.237 ± 0.028

4.441 ± 0.027

6.451 ± 0.036

6.279 ± 0.026

5.476 ± 0.034

6.889 ± 0.029

1.534 ± 0.017

2.566 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski