Beihai sobemo-like virus 25

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE85|A0A1L3KE85_9VIRU Capsid protein OS=Beihai sobemo-like virus 25 OX=1922697 PE=3 SV=1
MM1 pKa = 8.07DD2 pKa = 4.1SMGPRR7 pKa = 11.84WRR9 pKa = 11.84SKK11 pKa = 7.68QQYY14 pKa = 10.29VDD16 pKa = 4.48FDD18 pKa = 4.52DD19 pKa = 5.36DD20 pKa = 4.22RR21 pKa = 11.84YY22 pKa = 10.75HH23 pKa = 6.1RR24 pKa = 11.84HH25 pKa = 5.54SYY27 pKa = 10.27EE28 pKa = 3.77NASIAGSVTRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84SSKK44 pKa = 10.55KK45 pKa = 8.74EE46 pKa = 3.64RR47 pKa = 11.84QRR49 pKa = 11.84PATKK53 pKa = 10.08DD54 pKa = 3.14RR55 pKa = 11.84PSEE58 pKa = 3.8VHH60 pKa = 5.84YY61 pKa = 10.78KK62 pKa = 10.15IVGKK66 pKa = 10.04GKK68 pKa = 9.17PVHH71 pKa = 6.34GPSAPKK77 pKa = 8.85MQPEE81 pKa = 4.24ALQVIEE87 pKa = 4.19DD88 pKa = 3.76HH89 pKa = 6.67KK90 pKa = 11.45EE91 pKa = 3.75EE92 pKa = 4.02IVALGFEE99 pKa = 4.62EE100 pKa = 6.09GVFAYY105 pKa = 9.97PDD107 pKa = 3.53MSPDD111 pKa = 3.58TEE113 pKa = 4.14RR114 pKa = 11.84KK115 pKa = 9.92SLEE118 pKa = 3.45AHH120 pKa = 6.26LNLFGEE126 pKa = 4.6RR127 pKa = 11.84TRR129 pKa = 11.84TITHH133 pKa = 7.32PPTDD137 pKa = 4.75DD138 pKa = 2.87EE139 pKa = 4.42HH140 pKa = 6.72QRR142 pKa = 11.84CSRR145 pKa = 11.84LVAEE149 pKa = 5.1MMQSATFVPKK159 pKa = 9.87SDD161 pKa = 3.36YY162 pKa = 11.06RR163 pKa = 11.84EE164 pKa = 3.82VSGVLDD170 pKa = 4.93IINSSIIDD178 pKa = 3.6PKK180 pKa = 10.44KK181 pKa = 9.99ASGYY185 pKa = 9.15PYY187 pKa = 10.26CLEE190 pKa = 4.21GKK192 pKa = 6.76PTNGQVLEE200 pKa = 4.2QYY202 pKa = 9.98GVRR205 pKa = 11.84GFAQHH210 pKa = 6.78VLNTWEE216 pKa = 4.77DD217 pKa = 3.68LPFQVKK223 pKa = 10.13NFLKK227 pKa = 10.9GEE229 pKa = 4.12PTKK232 pKa = 9.97KK233 pKa = 8.99TKK235 pKa = 10.23LDD237 pKa = 3.32KK238 pKa = 11.21GMPRR242 pKa = 11.84CIEE245 pKa = 4.11GFPLHH250 pKa = 5.84VTVKK254 pKa = 9.2HH255 pKa = 5.93ASVFSQLAMTLVKK268 pKa = 9.55QWKK271 pKa = 8.96HH272 pKa = 5.78IPVKK276 pKa = 10.68YY277 pKa = 10.31AFSPANPGHH286 pKa = 6.41IEE288 pKa = 3.92HH289 pKa = 7.0LKK291 pKa = 9.61EE292 pKa = 4.06CLPGKK297 pKa = 9.85VWEE300 pKa = 4.24SDD302 pKa = 3.18KK303 pKa = 11.55TNWDD307 pKa = 3.36YY308 pKa = 12.12LMYY311 pKa = 10.64LWIANVVRR319 pKa = 11.84DD320 pKa = 4.24SIKK323 pKa = 10.83KK324 pKa = 10.05LVIKK328 pKa = 9.53PAEE331 pKa = 4.09WTEE334 pKa = 4.01EE335 pKa = 3.96QYY337 pKa = 11.24SIYY340 pKa = 10.68LSDD343 pKa = 3.76IDD345 pKa = 4.51GCFKK349 pKa = 10.78QVFEE353 pKa = 4.21EE354 pKa = 4.08ASYY357 pKa = 8.92RR358 pKa = 11.84TSDD361 pKa = 3.11GHH363 pKa = 7.85IYY365 pKa = 10.26QSNEE369 pKa = 3.45PGIMKK374 pKa = 10.14SGWFMTIGANSIAQVAVHH392 pKa = 6.23VMTCIRR398 pKa = 11.84LGMSDD403 pKa = 5.61DD404 pKa = 5.81DD405 pKa = 4.1ILSTPIVAGGDD416 pKa = 3.73DD417 pKa = 4.03VNQAPVPAGKK427 pKa = 9.68AAYY430 pKa = 10.15LEE432 pKa = 4.34EE433 pKa = 4.13AQKK436 pKa = 11.0LGVSMEE442 pKa = 3.54IHH444 pKa = 6.11EE445 pKa = 5.11RR446 pKa = 11.84GDD448 pKa = 3.66LYY450 pKa = 10.89EE451 pKa = 4.67SEE453 pKa = 4.74YY454 pKa = 10.84FSSDD458 pKa = 2.6LRR460 pKa = 11.84LGVEE464 pKa = 4.35GPEE467 pKa = 4.05FFPKK471 pKa = 9.81RR472 pKa = 11.84WTKK475 pKa = 10.53HH476 pKa = 4.69IEE478 pKa = 3.67HH479 pKa = 7.14LKK481 pKa = 7.98TVKK484 pKa = 10.45RR485 pKa = 11.84EE486 pKa = 3.98NLADD490 pKa = 3.64ALVSHH495 pKa = 6.5MEE497 pKa = 4.13NYY499 pKa = 9.33RR500 pKa = 11.84HH501 pKa = 6.52HH502 pKa = 6.92VDD504 pKa = 4.17KK505 pKa = 11.24FNLLVKK511 pKa = 10.43LYY513 pKa = 10.89LSLEE517 pKa = 4.21EE518 pKa = 5.22KK519 pKa = 10.93YY520 pKa = 10.43PADD523 pKa = 3.77FPKK526 pKa = 10.76SKK528 pKa = 10.38LVSRR532 pKa = 11.84SLLRR536 pKa = 11.84ARR538 pKa = 11.84QYY540 pKa = 11.25GYY542 pKa = 10.94EE543 pKa = 4.03HH544 pKa = 7.46ALLCC548 pKa = 5.34

Molecular weight: 62.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE90|A0A1L3KE90_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 25 OX=1922697 PE=4 SV=1
MM1 pKa = 7.34NNSLTTQYY9 pKa = 11.32LGIPGEE15 pKa = 5.02DD16 pKa = 3.5PTSPPWASGNYY27 pKa = 9.33VGPFWSNGKK36 pKa = 8.68LQEE39 pKa = 4.24SVEE42 pKa = 4.39FGDD45 pKa = 5.04APPLHH50 pKa = 6.74EE51 pKa = 5.65LDD53 pKa = 3.66ALARR57 pKa = 11.84LHH59 pKa = 6.09DD60 pKa = 3.76TAYY63 pKa = 10.78ARR65 pKa = 11.84YY66 pKa = 9.22KK67 pKa = 10.92DD68 pKa = 3.75SAHH71 pKa = 6.8RR72 pKa = 11.84AAADD76 pKa = 3.37EE77 pKa = 4.36LFAEE81 pKa = 4.78EE82 pKa = 5.04AEE84 pKa = 4.32KK85 pKa = 10.89LKK87 pKa = 10.89KK88 pKa = 10.35KK89 pKa = 10.68YY90 pKa = 10.15GPNWAGNPQVAAKK103 pKa = 9.91LVRR106 pKa = 11.84YY107 pKa = 9.28GNHH110 pKa = 6.51TIRR113 pKa = 11.84AASRR117 pKa = 11.84VGGNVATGFKK127 pKa = 10.61FGGPLGAVGGLLYY140 pKa = 10.92SGLQNIKK147 pKa = 9.87QSHH150 pKa = 6.48DD151 pKa = 3.62MVTGNYY157 pKa = 9.02LRR159 pKa = 11.84KK160 pKa = 9.37EE161 pKa = 4.12RR162 pKa = 11.84KK163 pKa = 9.52DD164 pKa = 3.55LLSLFSKK171 pKa = 10.99DD172 pKa = 3.07PMKK175 pKa = 10.28TIKK178 pKa = 9.94VQPIGGSVVEE188 pKa = 4.93PGSSDD193 pKa = 3.42SKK195 pKa = 10.37PKK197 pKa = 10.64KK198 pKa = 8.52KK199 pKa = 8.95TLVEE203 pKa = 3.94KK204 pKa = 10.78LKK206 pKa = 11.12NLVTSPTKK214 pKa = 9.76IVPVKK219 pKa = 10.39EE220 pKa = 4.19EE221 pKa = 3.81EE222 pKa = 4.38GRR224 pKa = 11.84PAWAVKK230 pKa = 8.41QSRR233 pKa = 11.84KK234 pKa = 9.8LEE236 pKa = 4.08KK237 pKa = 10.13FRR239 pKa = 11.84QRR241 pKa = 11.84LNDD244 pKa = 3.25ANNRR248 pKa = 11.84QKK250 pKa = 11.23AGLPPKK256 pKa = 10.21RR257 pKa = 11.84KK258 pKa = 9.3PKK260 pKa = 9.96RR261 pKa = 11.84KK262 pKa = 9.33KK263 pKa = 10.17NLGAALPDD271 pKa = 3.56AYY273 pKa = 9.82KK274 pKa = 10.2EE275 pKa = 4.1RR276 pKa = 11.84KK277 pKa = 8.02RR278 pKa = 11.84QEE280 pKa = 3.75VEE282 pKa = 3.79RR283 pKa = 11.84KK284 pKa = 9.62RR285 pKa = 11.84KK286 pKa = 8.87EE287 pKa = 3.69KK288 pKa = 10.32RR289 pKa = 11.84KK290 pKa = 10.38KK291 pKa = 9.84ILLKK295 pKa = 10.81VGGRR299 pKa = 11.84SAA301 pKa = 4.04

Molecular weight: 33.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1225

301

548

408.3

45.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.755 ± 0.905

0.898 ± 0.287

4.98 ± 0.456

5.714 ± 1.418

3.673 ± 0.566

6.939 ± 0.657

2.531 ± 0.889

4.327 ± 0.636

7.673 ± 1.981

7.592 ± 0.647

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.386

4.327 ± 0.865

6.122 ± 0.267

3.429 ± 0.119

5.469 ± 0.551

8.163 ± 1.069

6.041 ± 1.835

6.857 ± 0.274

1.551 ± 0.071

3.837 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski