Amino acid dipepetide frequency for Novosphingobium sp. MD-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.246AlaAla: 19.246 ± 0.195
1.129AlaCys: 1.129 ± 0.035
7.921AlaAsp: 7.921 ± 0.076
7.585AlaGlu: 7.585 ± 0.091
4.639AlaPhe: 4.639 ± 0.059
12.412AlaGly: 12.412 ± 0.117
2.503AlaHis: 2.503 ± 0.044
6.639AlaIle: 6.639 ± 0.065
3.63AlaLys: 3.63 ± 0.064
14.529AlaLeu: 14.529 ± 0.154
4.137AlaMet: 4.137 ± 0.056
3.194AlaAsn: 3.194 ± 0.059
6.834AlaPro: 6.834 ± 0.097
4.657AlaGln: 4.657 ± 0.064
10.599AlaArg: 10.599 ± 0.102
6.914AlaSer: 6.914 ± 0.08
6.672AlaThr: 6.672 ± 0.09
9.14AlaVal: 9.14 ± 0.103
1.783AlaTrp: 1.783 ± 0.04
2.64AlaTyr: 2.64 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.148CysAla: 1.148 ± 0.035
0.106CysCys: 0.106 ± 0.008
0.543CysAsp: 0.543 ± 0.019
0.392CysGlu: 0.392 ± 0.019
0.287CysPhe: 0.287 ± 0.015
0.931CysGly: 0.931 ± 0.03
0.268CysHis: 0.268 ± 0.018
0.353CysIle: 0.353 ± 0.018
0.177CysLys: 0.177 ± 0.012
0.766CysLeu: 0.766 ± 0.023
0.16CysMet: 0.16 ± 0.011
0.208CysAsn: 0.208 ± 0.012
0.496CysPro: 0.496 ± 0.027
0.225CysGln: 0.225 ± 0.014
0.558CysArg: 0.558 ± 0.019
0.455CysSer: 0.455 ± 0.022
0.414CysThr: 0.414 ± 0.018
0.574CysVal: 0.574 ± 0.021
0.118CysTrp: 0.118 ± 0.009
0.188CysTyr: 0.188 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.911AspAla: 7.911 ± 0.091
0.472AspCys: 0.472 ± 0.021
3.121AspAsp: 3.121 ± 0.055
3.186AspGlu: 3.186 ± 0.057
2.222AspPhe: 2.222 ± 0.043
5.676AspGly: 5.676 ± 0.079
1.364AspHis: 1.364 ± 0.034
2.714AspIle: 2.714 ± 0.045
1.607AspLys: 1.607 ± 0.037
5.801AspLeu: 5.801 ± 0.084
1.284AspMet: 1.284 ± 0.03
1.318AspAsn: 1.318 ± 0.034
3.844AspPro: 3.844 ± 0.055
1.636AspGln: 1.636 ± 0.036
4.795AspArg: 4.795 ± 0.063
2.231AspSer: 2.231 ± 0.043
2.628AspThr: 2.628 ± 0.058
4.14AspVal: 4.14 ± 0.061
1.121AspTrp: 1.121 ± 0.03
1.592AspTyr: 1.592 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
7.663GluAla: 7.663 ± 0.091
0.367GluCys: 0.367 ± 0.019
2.687GluAsp: 2.687 ± 0.05
2.768GluGlu: 2.768 ± 0.06
1.47GluPhe: 1.47 ± 0.034
4.404GluGly: 4.404 ± 0.059
1.127GluHis: 1.127 ± 0.032
2.674GluIle: 2.674 ± 0.046
1.813GluLys: 1.813 ± 0.04
4.811GluLeu: 4.811 ± 0.062
1.305GluMet: 1.305 ± 0.03
1.332GluAsn: 1.332 ± 0.032
2.513GluPro: 2.513 ± 0.046
2.109GluGln: 2.109 ± 0.044
4.711GluArg: 4.711 ± 0.069
2.155GluSer: 2.155 ± 0.039
3.04GluThr: 3.04 ± 0.045
3.411GluVal: 3.411 ± 0.052
0.763GluTrp: 0.763 ± 0.023
0.945GluTyr: 0.945 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.939PheAla: 4.939 ± 0.068
0.355PheCys: 0.355 ± 0.015
2.776PheAsp: 2.776 ± 0.051
1.816PheGlu: 1.816 ± 0.037
1.284PhePhe: 1.284 ± 0.036
3.799PheGly: 3.799 ± 0.06
0.794PheHis: 0.794 ± 0.024
1.382PheIle: 1.382 ± 0.03
0.815PheLys: 0.815 ± 0.023
3.109PheLeu: 3.109 ± 0.049
0.728PheMet: 0.728 ± 0.023
1.087PheAsn: 1.087 ± 0.029
1.616PhePro: 1.616 ± 0.034
0.867PheGln: 0.867 ± 0.027
2.368PheArg: 2.368 ± 0.043
2.023PheSer: 2.023 ± 0.037
2.097PheThr: 2.097 ± 0.044
2.664PheVal: 2.664 ± 0.046
0.516PheTrp: 0.516 ± 0.021
0.937PheTyr: 0.937 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.603GlyAla: 10.603 ± 0.107
0.868GlyCys: 0.868 ± 0.027
4.78GlyAsp: 4.78 ± 0.07
4.742GlyGlu: 4.742 ± 0.065
3.719GlyPhe: 3.719 ± 0.054
8.575GlyGly: 8.575 ± 0.136
1.969GlyHis: 1.969 ± 0.039
4.423GlyIle: 4.423 ± 0.058
3.372GlyLys: 3.372 ± 0.059
8.881GlyLeu: 8.881 ± 0.088
2.422GlyMet: 2.422 ± 0.042
2.438GlyAsn: 2.438 ± 0.063
3.864GlyPro: 3.864 ± 0.06
3.144GlyGln: 3.144 ± 0.049
6.476GlyArg: 6.476 ± 0.079
4.846GlySer: 4.846 ± 0.08
5.246GlyThr: 5.246 ± 0.091
6.515GlyVal: 6.515 ± 0.073
1.712GlyTrp: 1.712 ± 0.033
2.493GlyTyr: 2.493 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
2.719HisAla: 2.719 ± 0.047
0.237HisCys: 0.237 ± 0.014
1.272HisAsp: 1.272 ± 0.032
1.044HisGlu: 1.044 ± 0.027
0.867HisPhe: 0.867 ± 0.027
2.083HisGly: 2.083 ± 0.043
0.573HisHis: 0.573 ± 0.025
0.916HisIle: 0.916 ± 0.027
0.433HisLys: 0.433 ± 0.02
1.928HisLeu: 1.928 ± 0.041
0.485HisMet: 0.485 ± 0.018
0.45HisAsn: 0.45 ± 0.019
1.4HisPro: 1.4 ± 0.032
0.553HisGln: 0.553 ± 0.02
1.547HisArg: 1.547 ± 0.04
0.877HisSer: 0.877 ± 0.027
0.824HisThr: 0.824 ± 0.026
1.558HisVal: 1.558 ± 0.031
0.345HisTrp: 0.345 ± 0.016
0.563HisTyr: 0.563 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.867IleAla: 7.867 ± 0.082
0.439IleCys: 0.439 ± 0.019
3.841IleAsp: 3.841 ± 0.057
3.133IleGlu: 3.133 ± 0.058
1.315IlePhe: 1.315 ± 0.034
4.888IleGly: 4.888 ± 0.065
0.877IleHis: 0.877 ± 0.028
1.637IleIle: 1.637 ± 0.037
1.117IleLys: 1.117 ± 0.033
3.469IleLeu: 3.469 ± 0.055
0.821IleMet: 0.821 ± 0.026
1.313IleAsn: 1.313 ± 0.039
2.163IlePro: 2.163 ± 0.037
1.094IleGln: 1.094 ± 0.031
3.054IleArg: 3.054 ± 0.052
2.38IleSer: 2.38 ± 0.051
2.477IleThr: 2.477 ± 0.045
4.035IleVal: 4.035 ± 0.053
0.557IleTrp: 0.557 ± 0.023
0.972IleTyr: 0.972 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
4.079LysAla: 4.079 ± 0.065
0.137LysCys: 0.137 ± 0.01
1.457LysAsp: 1.457 ± 0.037
1.123LysGlu: 1.123 ± 0.033
0.813LysPhe: 0.813 ± 0.026
2.623LysGly: 2.623 ± 0.046
0.503LysHis: 0.503 ± 0.021
1.273LysIle: 1.273 ± 0.034
0.873LysLys: 0.873 ± 0.028
2.913LysLeu: 2.913 ± 0.053
0.643LysMet: 0.643 ± 0.024
0.656LysAsn: 0.656 ± 0.022
1.898LysPro: 1.898 ± 0.044
0.859LysGln: 0.859 ± 0.032
1.967LysArg: 1.967 ± 0.043
1.3LysSer: 1.3 ± 0.034
1.468LysThr: 1.468 ± 0.038
2.232LysVal: 2.232 ± 0.046
0.373LysTrp: 0.373 ± 0.019
0.566LysTyr: 0.566 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.145LeuAla: 15.145 ± 0.139
0.851LeuCys: 0.851 ± 0.027
6.003LeuAsp: 6.003 ± 0.076
4.817LeuGlu: 4.817 ± 0.071
3.557LeuPhe: 3.557 ± 0.062
8.479LeuGly: 8.479 ± 0.094
1.96LeuHis: 1.96 ± 0.041
4.307LeuIle: 4.307 ± 0.078
2.726LeuLys: 2.726 ± 0.047
9.337LeuLeu: 9.337 ± 0.124
2.036LeuMet: 2.036 ± 0.046
2.314LeuAsn: 2.314 ± 0.051
5.863LeuPro: 5.863 ± 0.07
2.438LeuGln: 2.438 ± 0.045
7.18LeuArg: 7.18 ± 0.079
5.725LeuSer: 5.725 ± 0.064
5.492LeuThr: 5.492 ± 0.074
7.407LeuVal: 7.407 ± 0.084
1.225LeuTrp: 1.225 ± 0.031
1.885LeuTyr: 1.885 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
3.452MetAla: 3.452 ± 0.052
0.162MetCys: 0.162 ± 0.01
1.158MetAsp: 1.158 ± 0.032
1.06MetGlu: 1.06 ± 0.025
0.66MetPhe: 0.66 ± 0.023
1.83MetGly: 1.83 ± 0.037
0.454MetHis: 0.454 ± 0.02
1.287MetIle: 1.287 ± 0.031
0.819MetLys: 0.819 ± 0.023
2.566MetLeu: 2.566 ± 0.045
0.604MetMet: 0.604 ± 0.024
0.698MetAsn: 0.698 ± 0.023
1.509MetPro: 1.509 ± 0.033
0.701MetGln: 0.701 ± 0.022
1.736MetArg: 1.736 ± 0.034
1.222MetSer: 1.222 ± 0.027
1.632MetThr: 1.632 ± 0.038
1.651MetVal: 1.651 ± 0.032
0.219MetTrp: 0.219 ± 0.013
0.26MetTyr: 0.26 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.452AsnAla: 3.452 ± 0.063
0.255AsnCys: 0.255 ± 0.016
1.358AsnAsp: 1.358 ± 0.04
1.046AsnGlu: 1.046 ± 0.033
1.011AsnPhe: 1.011 ± 0.032
2.585AsnGly: 2.585 ± 0.064
0.477AsnHis: 0.477 ± 0.019
1.192AsnIle: 1.192 ± 0.032
0.606AsnLys: 0.606 ± 0.024
2.501AsnLeu: 2.501 ± 0.041
0.523AsnMet: 0.523 ± 0.018
0.711AsnAsn: 0.711 ± 0.027
1.785AsnPro: 1.785 ± 0.042
0.73AsnGln: 0.73 ± 0.025
1.868AsnArg: 1.868 ± 0.038
1.188AsnSer: 1.188 ± 0.037
1.304AsnThr: 1.304 ± 0.032
1.895AsnVal: 1.895 ± 0.047
0.416AsnTrp: 0.416 ± 0.017
0.703AsnTyr: 0.703 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
7.918ProAla: 7.918 ± 0.098
0.352ProCys: 0.352 ± 0.015
3.907ProAsp: 3.907 ± 0.053
3.513ProGlu: 3.513 ± 0.054
2.02ProPhe: 2.02 ± 0.041
5.136ProGly: 5.136 ± 0.068
1.068ProHis: 1.068 ± 0.029
2.278ProIle: 2.278 ± 0.042
1.351ProLys: 1.351 ± 0.035
5.162ProLeu: 5.162 ± 0.072
1.188ProMet: 1.188 ± 0.03
1.222ProAsn: 1.222 ± 0.034
2.912ProPro: 2.912 ± 0.069
1.769ProGln: 1.769 ± 0.037
3.26ProArg: 3.26 ± 0.056
2.685ProSer: 2.685 ± 0.047
2.542ProThr: 2.542 ± 0.046
4.714ProVal: 4.714 ± 0.064
0.736ProTrp: 0.736 ± 0.023
1.131ProTyr: 1.131 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.412GlnAla: 4.412 ± 0.06
0.235GlnCys: 0.235 ± 0.014
1.476GlnAsp: 1.476 ± 0.034
1.318GlnGlu: 1.318 ± 0.034
1.104GlnPhe: 1.104 ± 0.028
2.714GlnGly: 2.714 ± 0.048
0.654GlnHis: 0.654 ± 0.021
1.68GlnIle: 1.68 ± 0.036
0.87GlnLys: 0.87 ± 0.029
2.853GlnLeu: 2.853 ± 0.05
0.741GlnMet: 0.741 ± 0.023
0.758GlnAsn: 0.758 ± 0.03
1.87GlnPro: 1.87 ± 0.041
1.152GlnGln: 1.152 ± 0.034
2.438GlnArg: 2.438 ± 0.05
1.613GlnSer: 1.613 ± 0.035
1.604GlnThr: 1.604 ± 0.036
2.354GlnVal: 2.354 ± 0.041
0.464GlnTrp: 0.464 ± 0.02
0.651GlnTyr: 0.651 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
9.309ArgAla: 9.309 ± 0.111
0.556ArgCys: 0.556 ± 0.021
4.242ArgAsp: 4.242 ± 0.069
4.071ArgGlu: 4.071 ± 0.063
3.2ArgPhe: 3.2 ± 0.056
5.204ArgGly: 5.204 ± 0.07
1.803ArgHis: 1.803 ± 0.043
4.112ArgIle: 4.112 ± 0.058
2.157ArgLys: 2.157 ± 0.043
8.25ArgLeu: 8.25 ± 0.104
1.943ArgMet: 1.943 ± 0.038
1.979ArgAsn: 1.979 ± 0.037
3.697ArgPro: 3.697 ± 0.059
2.691ArgGln: 2.691 ± 0.044
5.681ArgArg: 5.681 ± 0.087
3.469ArgSer: 3.469 ± 0.053
3.5ArgThr: 3.5 ± 0.055
4.974ArgVal: 4.974 ± 0.064
1.239ArgTrp: 1.239 ± 0.03
1.852ArgTyr: 1.852 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.562SerAla: 6.562 ± 0.086
0.44SerCys: 0.44 ± 0.02
2.914SerAsp: 2.914 ± 0.052
2.342SerGlu: 2.342 ± 0.044
2.0SerPhe: 2.0 ± 0.042
5.479SerGly: 5.479 ± 0.081
0.97SerHis: 0.97 ± 0.027
2.487SerIle: 2.487 ± 0.045
1.212SerLys: 1.212 ± 0.034
5.045SerLeu: 5.045 ± 0.067
1.11SerMet: 1.11 ± 0.032
1.34SerAsn: 1.34 ± 0.034
2.869SerPro: 2.869 ± 0.052
1.509SerGln: 1.509 ± 0.042
3.422SerArg: 3.422 ± 0.048
2.731SerSer: 2.731 ± 0.059
2.553SerThr: 2.553 ± 0.045
3.619SerVal: 3.619 ± 0.053
0.766SerTrp: 0.766 ± 0.025
1.338SerTyr: 1.338 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
6.602ThrAla: 6.602 ± 0.074
0.419ThrCys: 0.419 ± 0.026
2.764ThrAsp: 2.764 ± 0.053
2.229ThrGlu: 2.229 ± 0.036
1.899ThrPhe: 1.899 ± 0.04
5.535ThrGly: 5.535 ± 0.073
0.978ThrHis: 0.978 ± 0.026
2.836ThrIle: 2.836 ± 0.052
1.152ThrLys: 1.152 ± 0.028
5.784ThrLeu: 5.784 ± 0.075
1.16ThrMet: 1.16 ± 0.032
1.34ThrAsn: 1.34 ± 0.041
3.593ThrPro: 3.593 ± 0.054
1.428ThrGln: 1.428 ± 0.036
3.577ThrArg: 3.577 ± 0.051
2.662ThrSer: 2.662 ± 0.061
2.663ThrThr: 2.663 ± 0.06
4.373ThrVal: 4.373 ± 0.057
0.649ThrTrp: 0.649 ± 0.023
1.189ThrTyr: 1.189 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
9.581ValAla: 9.581 ± 0.09
0.628ValCys: 0.628 ± 0.024
4.251ValAsp: 4.251 ± 0.058
4.204ValGlu: 4.204 ± 0.062
2.444ValPhe: 2.444 ± 0.049
5.552ValGly: 5.552 ± 0.07
1.398ValHis: 1.398 ± 0.036
3.763ValIle: 3.763 ± 0.055
1.983ValLys: 1.983 ± 0.04
7.188ValLeu: 7.188 ± 0.08
1.643ValMet: 1.643 ± 0.033
2.109ValAsn: 2.109 ± 0.051
4.311ValPro: 4.311 ± 0.062
2.03ValGln: 2.03 ± 0.037
5.405ValArg: 5.405 ± 0.072
4.142ValSer: 4.142 ± 0.064
4.693ValThr: 4.693 ± 0.066
5.405ValVal: 5.405 ± 0.079
0.933ValTrp: 0.933 ± 0.023
1.384ValTyr: 1.384 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.358TrpAla: 1.358 ± 0.031
0.135TrpCys: 0.135 ± 0.01
0.717TrpAsp: 0.717 ± 0.024
0.605TrpGlu: 0.605 ± 0.02
0.59TrpPhe: 0.59 ± 0.022
1.011TrpGly: 1.011 ± 0.025
0.396TrpHis: 0.396 ± 0.016
0.672TrpIle: 0.672 ± 0.022
0.465TrpLys: 0.465 ± 0.021
1.776TrpLeu: 1.776 ± 0.04
0.342TrpMet: 0.342 ± 0.016
0.49TrpAsn: 0.49 ± 0.02
0.719TrpPro: 0.719 ± 0.025
0.683TrpGln: 0.683 ± 0.025
1.363TrpArg: 1.363 ± 0.031
0.953TrpSer: 0.953 ± 0.027
0.868TrpThr: 0.868 ± 0.027
0.841TrpVal: 0.841 ± 0.026
0.255TrpTrp: 0.255 ± 0.014
0.304TrpTyr: 0.304 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.838TyrAla: 2.838 ± 0.048
0.223TyrCys: 0.223 ± 0.014
1.475TyrAsp: 1.475 ± 0.039
1.071TyrGlu: 1.071 ± 0.03
0.871TyrPhe: 0.871 ± 0.029
2.174TyrGly: 2.174 ± 0.043
0.535TyrHis: 0.535 ± 0.02
0.824TyrIle: 0.824 ± 0.021
0.579TyrLys: 0.579 ± 0.026
2.078TyrLeu: 2.078 ± 0.048
0.406TyrMet: 0.406 ± 0.018
0.648TyrAsn: 0.648 ± 0.024
1.107TyrPro: 1.107 ± 0.028
0.687TyrGln: 0.687 ± 0.025
1.923TyrArg: 1.923 ± 0.04
1.142TyrSer: 1.142 ± 0.038
1.116TyrThr: 1.116 ± 0.036
1.547TyrVal: 1.547 ± 0.036
0.351TyrTrp: 0.351 ± 0.018
0.645TyrTyr: 0.645 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4161 proteins (1363674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski