Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Psathyrellaceae; Coprinopsis; Coprinopsis cinerea

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8NHD5|A8NHD5_COPC7 Uncharacterized protein OS=Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) OX=240176 GN=CC1G_11527 PE=4 SV=2
MM1 pKa = 6.81TTDD4 pKa = 3.14STSLFDD10 pKa = 4.15LARR13 pKa = 11.84NKK15 pKa = 10.5LQTSFGGHH23 pKa = 6.64KK24 pKa = 10.12DD25 pKa = 2.85RR26 pKa = 11.84CSLHH30 pKa = 6.24RR31 pKa = 11.84WVLLKK36 pKa = 10.99NSIINSTSALVSSAVDD52 pKa = 3.27AVSEE56 pKa = 4.46TQVSSYY62 pKa = 11.44SEE64 pKa = 5.02DD65 pKa = 4.37LDD67 pKa = 6.47DD68 pKa = 6.32DD69 pKa = 5.59DD70 pKa = 5.53SVSDD74 pKa = 3.82EE75 pKa = 4.33VLSSVAGGSVHH86 pKa = 7.63SFMFPDD92 pKa = 3.97AGNLVEE98 pKa = 5.7AGPADD103 pKa = 3.54AHH105 pKa = 6.73SSEE108 pKa = 4.27AQWLDD113 pKa = 3.3SLLEE117 pKa = 4.07ALGDD121 pKa = 4.95DD122 pKa = 5.06DD123 pKa = 7.11DD124 pKa = 7.39DD125 pKa = 6.35DD126 pKa = 5.38FGPDD130 pKa = 3.1SDD132 pKa = 5.44SRR134 pKa = 11.84TEE136 pKa = 5.39DD137 pKa = 3.13EE138 pKa = 5.26DD139 pKa = 3.68EE140 pKa = 4.31QLFSPSASPMSSSDD154 pKa = 3.68DD155 pKa = 4.03LLPSMHH161 pKa = 6.32NHH163 pKa = 6.64HH164 pKa = 7.48PFQQHH169 pKa = 3.47QHH171 pKa = 5.33HH172 pKa = 7.19SYY174 pKa = 10.54FSSLPVSYY182 pKa = 9.71PIIDD186 pKa = 4.87PYY188 pKa = 10.31PPCHH192 pKa = 6.66PPLASPYY199 pKa = 10.42AFDD202 pKa = 4.1SSSLSSLPPPYY213 pKa = 10.07EE214 pKa = 4.67DD215 pKa = 4.72PLPFSDD221 pKa = 5.93DD222 pKa = 3.45VDD224 pKa = 4.25DD225 pKa = 4.67MPVPDD230 pKa = 4.95TIEE233 pKa = 5.43DD234 pKa = 3.61NTSDD238 pKa = 4.94DD239 pKa = 4.48EE240 pKa = 4.96SDD242 pKa = 4.02SPPTPMGGSRR252 pKa = 11.84SSLFDD257 pKa = 3.42VDD259 pKa = 4.32VLDD262 pKa = 4.62AASIPLPPDD271 pKa = 3.34RR272 pKa = 11.84SRR274 pKa = 11.84LSHH277 pKa = 5.89GAAVRR282 pKa = 11.84VYY284 pKa = 10.14DD285 pKa = 3.9QDD287 pKa = 4.03SSSSSCFGPSFDD299 pKa = 4.92PLPFSTNDD307 pKa = 2.93VHH309 pKa = 6.57STYY312 pKa = 11.27NLYY315 pKa = 10.57SEE317 pKa = 4.91CC318 pKa = 4.87

Molecular weight: 34.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6RQP7|D6RQP7_COPC7 Uncharacterized protein OS=Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) OX=240176 GN=CC1G_15455 PE=4 SV=1
MM1 pKa = 7.56AVLDD5 pKa = 3.9VFGLVLKK12 pKa = 10.41GGRR15 pKa = 11.84RR16 pKa = 11.84VRR18 pKa = 11.84QPLTRR23 pKa = 11.84TRR25 pKa = 11.84TRR27 pKa = 11.84SLPRR31 pKa = 11.84TRR33 pKa = 11.84NPPGIEE39 pKa = 4.13NPCPSLDD46 pKa = 3.68VFSSLSGRR54 pKa = 11.84WGAQAEE60 pKa = 4.38RR61 pKa = 11.84CNDD64 pKa = 3.01SHH66 pKa = 7.25RR67 pKa = 11.84KK68 pKa = 9.01KK69 pKa = 10.03NHH71 pKa = 6.05PSAAYY76 pKa = 10.48SFFDD80 pKa = 3.86HH81 pKa = 6.87PTNSLRR87 pKa = 11.84QRR89 pKa = 11.84AWLVFSVGGLVRR101 pKa = 11.84YY102 pKa = 8.58AALRR106 pKa = 11.84KK107 pKa = 8.73VTMNARR113 pKa = 11.84GALASFTSGRR123 pKa = 11.84EE124 pKa = 3.85FGNWWCQGRR133 pKa = 11.84SPVDD137 pKa = 3.04AFAFILPKK145 pKa = 10.38MMVPSLKK152 pKa = 9.86TLVLEE157 pKa = 4.5TRR159 pKa = 11.84YY160 pKa = 9.98HH161 pKa = 6.26RR162 pKa = 11.84PALFAAIVHH171 pKa = 6.55EE172 pKa = 4.67DD173 pKa = 3.92SDD175 pKa = 4.14WEE177 pKa = 4.14TLDD180 pKa = 4.29FVSCRR185 pKa = 11.84VTMVQDD191 pKa = 3.49

Molecular weight: 21.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13334

1

13335

6160588

27

4998

462.0

51.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.129 ± 0.019

1.164 ± 0.009

5.653 ± 0.014

6.215 ± 0.022

3.654 ± 0.017

6.654 ± 0.022

2.493 ± 0.01

4.633 ± 0.018

4.76 ± 0.018

9.042 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.927 ± 0.008

3.517 ± 0.012

6.922 ± 0.029

3.767 ± 0.015

6.402 ± 0.02

8.745 ± 0.029

6.035 ± 0.013

6.232 ± 0.016

1.42 ± 0.009

2.636 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski