Bacillus phage PM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pemunavirus; Bacillus virus PM1

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4ZR48|M4ZR48_9CAUD Uncharacterized protein OS=Bacillus phage PM1 OX=547228 PE=4 SV=1
MM1 pKa = 6.9KK2 pKa = 10.29QIYY5 pKa = 10.14KK6 pKa = 10.18YY7 pKa = 10.01DD8 pKa = 3.46NEE10 pKa = 4.36MNYY13 pKa = 9.46VPYY16 pKa = 11.13GNMIINDD23 pKa = 3.82GDD25 pKa = 4.35NIPEE29 pKa = 4.94GYY31 pKa = 9.25TDD33 pKa = 4.23IPPVNPDD40 pKa = 2.63GSGMYY45 pKa = 10.22KK46 pKa = 10.25PVFDD50 pKa = 4.46KK51 pKa = 11.14DD52 pKa = 3.25KK53 pKa = 11.05SVWRR57 pKa = 11.84EE58 pKa = 3.59TATQEE63 pKa = 4.92YY64 pKa = 9.52IDD66 pKa = 4.58SLQPPPPEE74 pKa = 4.32PSEE77 pKa = 4.79LDD79 pKa = 3.12ILSEE83 pKa = 4.18KK84 pKa = 10.49VADD87 pKa = 3.9IYY89 pKa = 11.8YY90 pKa = 9.29MIAIGGG96 pKa = 3.74

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4ZR19|M4ZR19_9CAUD Uncharacterized protein OS=Bacillus phage PM1 OX=547228 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.27KK3 pKa = 10.2IILALVAVITLGVMVLPEE21 pKa = 4.17SASAALSGYY30 pKa = 7.31QTSKK34 pKa = 10.61KK35 pKa = 9.47VRR37 pKa = 11.84VYY39 pKa = 10.79TDD41 pKa = 2.55ATTYY45 pKa = 10.66SKK47 pKa = 11.07SATSVDD53 pKa = 2.62WKK55 pKa = 10.56AQKK58 pKa = 6.84TTSGKK63 pKa = 9.7VYY65 pKa = 9.69YY66 pKa = 10.35SAMLVRR72 pKa = 11.84TDD74 pKa = 3.45NYY76 pKa = 10.96SNSGTQRR83 pKa = 11.84GSFTSGTPLKK93 pKa = 10.44KK94 pKa = 10.43FSLSKK99 pKa = 9.64TRR101 pKa = 11.84PGTYY105 pKa = 8.95QVVVNIYY112 pKa = 8.51SDD114 pKa = 3.24SSEE117 pKa = 3.54RR118 pKa = 11.84HH119 pKa = 5.55YY120 pKa = 11.22IGTAKK125 pKa = 10.14SAKK128 pKa = 9.83IYY130 pKa = 10.32IKK132 pKa = 10.58RR133 pKa = 3.56

Molecular weight: 14.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

15253

50

1456

177.4

20.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.615 ± 0.377

0.636 ± 0.086

6.11 ± 0.177

8.064 ± 0.485

4.222 ± 0.206

6.884 ± 0.473

1.567 ± 0.14

6.904 ± 0.21

9.061 ± 0.418

7.166 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.16

5.815 ± 0.294

3.435 ± 0.219

3.606 ± 0.207

4.268 ± 0.254

5.592 ± 0.247

5.828 ± 0.198

6.196 ± 0.257

1.075 ± 0.091

4.006 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski