Sewage-associated circular DNA virus-34

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH99|A0A0B4UH99_9VIRU Coat protein OS=Sewage-associated circular DNA virus-34 OX=1592101 PE=4 SV=1
MM1 pKa = 7.6SKK3 pKa = 10.27PLRR6 pKa = 11.84NICFTSFNLDD16 pKa = 3.29IEE18 pKa = 4.46WFEE21 pKa = 4.49TEE23 pKa = 4.34WPSHH27 pKa = 5.76HH28 pKa = 7.01LPGEE32 pKa = 4.15EE33 pKa = 3.73TGMVQYY39 pKa = 10.57IIVQGEE45 pKa = 3.96FCKK48 pKa = 10.45EE49 pKa = 3.97GKK51 pKa = 9.69KK52 pKa = 10.05HH53 pKa = 4.84IQGYY57 pKa = 8.68AQLRR61 pKa = 11.84GQYY64 pKa = 8.14YY65 pKa = 10.08LKK67 pKa = 10.41KK68 pKa = 9.61IKK70 pKa = 10.43EE71 pKa = 4.1FFGDD75 pKa = 3.18NGLHH79 pKa = 5.03VEE81 pKa = 4.32PRR83 pKa = 11.84RR84 pKa = 11.84GTHH87 pKa = 4.7EE88 pKa = 3.87QARR91 pKa = 11.84DD92 pKa = 3.39YY93 pKa = 11.21CKK95 pKa = 10.16QEE97 pKa = 4.06KK98 pKa = 9.16NGRR101 pKa = 11.84WHH103 pKa = 7.7DD104 pKa = 3.93YY105 pKa = 11.54VEE107 pKa = 4.68MGDD110 pKa = 3.53QKK112 pKa = 11.42KK113 pKa = 10.17PGSRR117 pKa = 11.84TDD119 pKa = 3.61LLDD122 pKa = 4.13VIDD125 pKa = 5.01KK126 pKa = 10.37IKK128 pKa = 10.71EE129 pKa = 4.1GEE131 pKa = 4.31TIASIISNSRR141 pKa = 11.84DD142 pKa = 2.97PKK144 pKa = 8.44MVRR147 pKa = 11.84NAVVYY152 pKa = 10.66NRR154 pKa = 11.84TLTAIEE160 pKa = 4.25KK161 pKa = 9.29QFKK164 pKa = 9.44YY165 pKa = 10.69DD166 pKa = 3.49AAIKK170 pKa = 9.72EE171 pKa = 4.34AIKK174 pKa = 10.48EE175 pKa = 4.0YY176 pKa = 11.2DD177 pKa = 3.23GVTWRR182 pKa = 11.84KK183 pKa = 6.78WQEE186 pKa = 3.58EE187 pKa = 4.29LLCYY191 pKa = 9.92VDD193 pKa = 5.14KK194 pKa = 11.4DD195 pKa = 3.25ADD197 pKa = 3.53KK198 pKa = 11.25RR199 pKa = 11.84KK200 pKa = 9.42VRR202 pKa = 11.84WYY204 pKa = 9.73TDD206 pKa = 2.92MGGNTGKK213 pKa = 10.67SYY215 pKa = 9.13ITTYY219 pKa = 9.8LTLLGKK225 pKa = 10.46AYY227 pKa = 10.19VVSGGKK233 pKa = 9.07QADD236 pKa = 3.19ILYY239 pKa = 9.96AYY241 pKa = 8.71EE242 pKa = 4.38DD243 pKa = 4.04QPIVIYY249 pKa = 10.69DD250 pKa = 3.83LARR253 pKa = 11.84AYY255 pKa = 7.78EE256 pKa = 3.91QNMDD260 pKa = 4.17HH261 pKa = 7.06IYY263 pKa = 9.66VTMEE267 pKa = 3.53YY268 pKa = 10.17FKK270 pKa = 11.07NGRR273 pKa = 11.84FLSTKK278 pKa = 9.41YY279 pKa = 9.05EE280 pKa = 3.94SKK282 pKa = 9.73MRR284 pKa = 11.84VFKK287 pKa = 10.52RR288 pKa = 11.84PHH290 pKa = 6.2VIVMANFPPHH300 pKa = 6.3INKK303 pKa = 9.92LSEE306 pKa = 4.04DD307 pKa = 3.39RR308 pKa = 11.84WDD310 pKa = 3.27IVYY313 pKa = 7.48MTNPP317 pKa = 3.42

Molecular weight: 37.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UH99|A0A0B4UH99_9VIRU Coat protein OS=Sewage-associated circular DNA virus-34 OX=1592101 PE=4 SV=1
MM1 pKa = 7.4SLVKK5 pKa = 10.65YY6 pKa = 10.27RR7 pKa = 11.84ISKK10 pKa = 9.68PKK12 pKa = 7.77YY13 pKa = 7.23TKK15 pKa = 10.28KK16 pKa = 10.42KK17 pKa = 9.21SEE19 pKa = 4.02KK20 pKa = 9.94KK21 pKa = 8.46FTSKK25 pKa = 10.69RR26 pKa = 11.84SINTMLSRR34 pKa = 11.84LPEE37 pKa = 3.79TKK39 pKa = 10.61YY40 pKa = 10.43YY41 pKa = 10.8DD42 pKa = 3.59VYY44 pKa = 10.42HH45 pKa = 6.13TTTITQNGALDD56 pKa = 4.79LLTGMTTLGTGEE68 pKa = 4.14GQRR71 pKa = 11.84VGSKK75 pKa = 10.23VFVKK79 pKa = 10.0YY80 pKa = 10.33IQVNGLIMRR89 pKa = 11.84NASSTIDD96 pKa = 3.36NLRR99 pKa = 11.84IIIFMDD105 pKa = 3.31KK106 pKa = 10.83QGMNTPAVTDD116 pKa = 3.41IMEE119 pKa = 4.49PAYY122 pKa = 10.38LGSGFSPVGRR132 pKa = 11.84INEE135 pKa = 3.95YY136 pKa = 10.31RR137 pKa = 11.84IPRR140 pKa = 11.84YY141 pKa = 9.89KK142 pKa = 9.99ILYY145 pKa = 9.29DD146 pKa = 3.46KK147 pKa = 9.46LTHH150 pKa = 6.98VSTQKK155 pKa = 10.96DD156 pKa = 3.56GFNFKK161 pKa = 11.16ANLRR165 pKa = 11.84INMPVYY171 pKa = 10.4YY172 pKa = 10.54VSTSTFKK179 pKa = 10.44NQVYY183 pKa = 10.47ILFLGDD189 pKa = 3.37NGNVLTLPYY198 pKa = 10.12CNYY201 pKa = 7.71TARR204 pKa = 11.84VSYY207 pKa = 10.52TDD209 pKa = 3.08QQ210 pKa = 4.44

Molecular weight: 23.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

527

210

317

263.5

30.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.985 ± 0.622

0.949 ± 0.26

5.693 ± 0.775

5.503 ± 1.721

3.605 ± 0.113

6.641 ± 0.014

2.087 ± 0.626

7.021 ± 0.067

9.108 ± 0.296

6.641 ± 0.801

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.416 ± 0.217

5.123 ± 0.851

3.605 ± 0.113

3.795 ± 0.254

5.503 ± 0.146

5.123 ± 1.113

7.4 ± 1.695

6.262 ± 0.223

1.328 ± 0.732

7.211 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski