Pseudomonas pharmacofabricae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseu

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0CTT0|A0A2I0CTT0_9PSED Histidine kinase OS=Pseudomonas pharmacofabricae OX=1905276 GN=CW360_03385 PE=4 SV=1
MM1 pKa = 6.96STSNTRR7 pKa = 11.84QINGVTAAYY16 pKa = 10.26SPSQEE21 pKa = 3.79FRR23 pKa = 11.84FFLYY27 pKa = 10.59DD28 pKa = 3.31PEE30 pKa = 6.02GFDD33 pKa = 3.54FLYY36 pKa = 10.55FRR38 pKa = 11.84SPEE41 pKa = 4.04DD42 pKa = 3.38RR43 pKa = 11.84NKK45 pKa = 10.68AAEE48 pKa = 4.16EE49 pKa = 4.29VISAYY54 pKa = 10.86LDD56 pKa = 4.34DD57 pKa = 4.4GWSEE61 pKa = 3.89EE62 pKa = 4.17VEE64 pKa = 3.95NVIAGEE70 pKa = 4.09ITHH73 pKa = 6.6TCDD76 pKa = 4.05KK77 pKa = 10.54INVVPRR83 pKa = 11.84PDD85 pKa = 3.58EE86 pKa = 4.14VDD88 pKa = 3.21EE89 pKa = 5.62DD90 pKa = 4.28GLDD93 pKa = 3.8GEE95 pKa = 4.7GNYY98 pKa = 8.38WAEE101 pKa = 4.05EE102 pKa = 4.11WDD104 pKa = 4.14CKK106 pKa = 10.48CDD108 pKa = 3.69YY109 pKa = 11.28GLTPVAAAADD119 pKa = 3.84EE120 pKa = 4.52TEE122 pKa = 3.93QCLEE126 pKa = 3.91QSAA129 pKa = 3.86

Molecular weight: 14.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0CN75|A0A2I0CN75_9PSED Uncharacterized protein OS=Pseudomonas pharmacofabricae OX=1905276 GN=CW360_12175 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3166

0

3166

1018675

27

2533

321.8

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.686 ± 0.054

1.123 ± 0.012

5.08 ± 0.028

5.949 ± 0.04

3.32 ± 0.029

7.68 ± 0.036

2.312 ± 0.022

4.319 ± 0.035

2.975 ± 0.041

13.036 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.021

2.673 ± 0.026

4.791 ± 0.029

5.76 ± 0.057

7.064 ± 0.038

5.584 ± 0.035

4.02 ± 0.031

6.588 ± 0.047

1.465 ± 0.02

2.457 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski