Arthrobacter phage Edmundo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Laroyevirus; unclassified Laroyevirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V8J0|A0A649V8J0_9CAUD Uncharacterized protein OS=Arthrobacter phage Edmundo OX=2656524 GN=40 PE=4 SV=1
MM1 pKa = 7.52AAKK4 pKa = 8.47THH6 pKa = 6.33QGDD9 pKa = 4.51CVDD12 pKa = 4.42IDD14 pKa = 3.78PFIPTGQCALCTDD27 pKa = 4.67TPQDD31 pKa = 3.83TIPPEE36 pKa = 4.28DD37 pKa = 3.89RR38 pKa = 11.84DD39 pKa = 3.88GGVQQ43 pKa = 3.24

Molecular weight: 4.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V6Y1|A0A649V6Y1_9CAUD Uncharacterized protein OS=Arthrobacter phage Edmundo OX=2656524 GN=2 PE=4 SV=1
MM1 pKa = 7.36TAPKK5 pKa = 9.6PRR7 pKa = 11.84PLKK10 pKa = 9.73PLPKK14 pKa = 9.09PSPPAGSRR22 pKa = 11.84AFDD25 pKa = 4.86DD26 pKa = 3.6MLQTYY31 pKa = 9.61YY32 pKa = 11.01EE33 pKa = 4.2MPGCRR38 pKa = 11.84EE39 pKa = 3.99LVNGIIEE46 pKa = 4.19RR47 pKa = 11.84KK48 pKa = 8.83RR49 pKa = 11.84ARR51 pKa = 11.84EE52 pKa = 3.67GAKK55 pKa = 10.02VVEE58 pKa = 4.51SRR60 pKa = 11.84ARR62 pKa = 11.84RR63 pKa = 3.46

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

18692

27

1005

190.7

20.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.802 ± 0.48

0.776 ± 0.125

5.623 ± 0.281

6.072 ± 0.326

2.557 ± 0.199

8.485 ± 0.332

2.108 ± 0.144

4.761 ± 0.283

4.157 ± 0.284

7.431 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.111

3.274 ± 0.161

5.719 ± 0.257

3.649 ± 0.24

6.152 ± 0.349

5.468 ± 0.281

7.42 ± 0.339

7.527 ± 0.215

1.862 ± 0.09

2.707 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski