Paramecium bursaria Chlorella virus 1 (PBCV-1)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q84614|Q84614_PBCV1 Uncharacterized protein OS=Paramecium bursaria Chlorella virus 1 OX=10506 GN=A298L PE=4 SV=1
MM1 pKa = 7.93SDD3 pKa = 3.25FCFATFMITEE13 pKa = 4.4EE14 pKa = 4.1PALGSSLNTLPPPLFSPILSCSMSEE39 pKa = 3.87AVTFALYY46 pKa = 10.58ADD48 pKa = 5.64PYY50 pKa = 8.51TPPPIFFPNLLGVNLISVDD69 pKa = 3.48TTLEE73 pKa = 3.67ILLASDD79 pKa = 3.12ILKK82 pKa = 10.27FFIGRR87 pKa = 11.84NVLGILL93 pKa = 3.79

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O41165|O41165_PBCV1 Cytosine-specific methyltransferase OS=Paramecium bursaria Chlorella virus 1 OX=10506 GN=A683L PE=3 SV=1
MM1 pKa = 7.37VMPHH5 pKa = 5.72FRR7 pKa = 11.84QRR9 pKa = 11.84NHH11 pKa = 5.65LQRR14 pKa = 11.84NHH16 pKa = 5.73LQRR19 pKa = 11.84NHH21 pKa = 5.73LQRR24 pKa = 11.84NHH26 pKa = 5.73LQRR29 pKa = 11.84NHH31 pKa = 5.73LQRR34 pKa = 11.84NHH36 pKa = 5.74LQRR39 pKa = 11.84NHH41 pKa = 5.88LRR43 pKa = 11.84RR44 pKa = 11.84NHH46 pKa = 5.8LRR48 pKa = 11.84RR49 pKa = 11.84NHH51 pKa = 5.8LRR53 pKa = 11.84RR54 pKa = 11.84NHH56 pKa = 5.8LRR58 pKa = 11.84RR59 pKa = 11.84NHH61 pKa = 5.77LRR63 pKa = 11.84RR64 pKa = 11.84NHH66 pKa = 4.44QHH68 pKa = 5.87LPEE71 pKa = 4.16VFKK74 pKa = 11.29

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

792

2

794

136866

40

1369

172.4

19.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.407 ± 0.115

1.907 ± 0.069

5.03 ± 0.109

4.997 ± 0.133

5.497 ± 0.111

5.567 ± 0.169

2.156 ± 0.072

7.363 ± 0.114

7.262 ± 0.2

7.796 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.062

5.812 ± 0.205

4.789 ± 0.174

2.732 ± 0.076

4.93 ± 0.129

7.442 ± 0.141

6.565 ± 0.145

7.092 ± 0.112

1.097 ± 0.046

3.785 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski