Iodobacter phage PhiPLPE

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Iodovirus; Iodobacter virus PLPE

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5AX69|B5AX69_9CAUD Uncharacterized protein OS=Iodobacter phage PhiPLPE OX=551895 GN=phiPLPE_50 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84PCVGVGGACPPLCTWAQLEE21 pKa = 4.41DD22 pKa = 3.76GTYY25 pKa = 10.76SLADD29 pKa = 3.04VEE31 pKa = 4.85MFNQALDD38 pKa = 3.9DD39 pKa = 4.4VIEE42 pKa = 4.88ANTPPQQ48 pKa = 3.35

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5AX27|B5AX27_9CAUD Uncharacterized protein OS=Iodobacter phage PhiPLPE OX=551895 GN=phiPLPE_08 PE=4 SV=1
MM1 pKa = 7.72SEE3 pKa = 3.92AKK5 pKa = 10.78YY6 pKa = 10.34FGAWDD11 pKa = 3.3QGKK14 pKa = 8.75SLRR17 pKa = 11.84GGTIAQTKK25 pKa = 8.5QVSPTALRR33 pKa = 11.84YY34 pKa = 7.41TCARR38 pKa = 11.84CNVFLGRR45 pKa = 11.84HH46 pKa = 4.99NEE48 pKa = 3.99APVCHH53 pKa = 6.58CGSTRR58 pKa = 11.84FHH60 pKa = 7.23SGRR63 pKa = 3.61

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

14877

45

483

177.1

19.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.036 ± 0.399

1.324 ± 0.121

6.231 ± 0.234

5.989 ± 0.328

3.482 ± 0.182

7.434 ± 0.324

1.264 ± 0.106

6.413 ± 0.2

6.392 ± 0.295

7.488 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.119

4.961 ± 0.222

3.811 ± 0.18

3.569 ± 0.2

4.591 ± 0.209

6.735 ± 0.295

5.902 ± 0.343

6.695 ± 0.217

1.492 ± 0.133

3.395 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski