Clostridium phage phi24R

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Guelinviridae; Denniswatsonvirinae; Gregsiragusavirus; Clostridium virus phi24R

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9J3I6|G9J3I6_9CAUD Uncharacterized protein OS=Clostridium phage phi24R OX=1128071 GN=phi24R_gp19 PE=4 SV=1
MM1 pKa = 8.26IIIDD5 pKa = 3.78RR6 pKa = 11.84NKK8 pKa = 9.0ITVNQFDD15 pKa = 4.73LCILDD20 pKa = 5.55LKK22 pKa = 10.43LCEE25 pKa = 4.25YY26 pKa = 9.82NLKK29 pKa = 10.81VGDD32 pKa = 4.38KK33 pKa = 10.64IEE35 pKa = 4.06INIDD39 pKa = 3.46GEE41 pKa = 4.5ITVQDD46 pKa = 4.33YY47 pKa = 11.79NNLDD51 pKa = 3.36IQFNTDD57 pKa = 2.69NKK59 pKa = 11.06GVFDD63 pKa = 3.91YY64 pKa = 10.93SITIIQQGFARR75 pKa = 11.84TKK77 pKa = 9.83VIQNKK82 pKa = 9.65IEE84 pKa = 4.14VVV86 pKa = 3.31

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9J3H1|G9J3H1_9CAUD Uncharacterized protein OS=Clostridium phage phi24R OX=1128071 GN=phi24R_gp4 PE=4 SV=1
MM1 pKa = 7.38GLKK4 pKa = 9.03TFHH7 pKa = 6.62AVVVEE12 pKa = 3.8PFYY15 pKa = 11.75YY16 pKa = 10.41EE17 pKa = 4.82LNSKK21 pKa = 9.98NYY23 pKa = 10.51NIGDD27 pKa = 4.5DD28 pKa = 3.58IFVIDD33 pKa = 4.5ILGDD37 pKa = 3.61KK38 pKa = 10.61YY39 pKa = 11.08FLEE42 pKa = 5.17SDD44 pKa = 4.08VIDD47 pKa = 4.55IIPRR51 pKa = 11.84KK52 pKa = 8.81NVRR55 pKa = 11.84KK56 pKa = 9.59VRR58 pKa = 11.84KK59 pKa = 9.26HH60 pKa = 4.92GKK62 pKa = 9.59KK63 pKa = 10.16KK64 pKa = 10.66INIKK68 pKa = 10.23KK69 pKa = 10.24

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

5948

51

769

270.4

31.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.194 ± 0.302

1.446 ± 0.225

6.086 ± 0.257

7.969 ± 0.584

4.657 ± 0.302

6.153 ± 0.642

1.143 ± 0.112

8.406 ± 0.554

9.516 ± 0.769

8.221 ± 0.491

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.155

9.465 ± 0.546

2.236 ± 0.28

2.808 ± 0.363

3.161 ± 0.208

6.069 ± 0.35

5.313 ± 0.263

5.851 ± 0.431

0.757 ± 0.141

5.262 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski