Didemnum sp. Sea Squirt associated virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL41|A0A0K1RL41_9CIRC Putative capsid protein OS=Didemnum sp. Sea Squirt associated virus OX=1692247 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 4.36NTTTKK7 pKa = 10.69HH8 pKa = 3.99EE9 pKa = 4.49TKK11 pKa = 10.2PATNLPHH18 pKa = 7.02KK19 pKa = 10.54SWFLTLNNWTEE30 pKa = 4.05TEE32 pKa = 4.15YY33 pKa = 11.13KK34 pKa = 10.16ILQQLHH40 pKa = 5.56GVTYY44 pKa = 10.39SIIGKK49 pKa = 8.79EE50 pKa = 4.34GKK52 pKa = 7.89EE53 pKa = 4.25TTPHH57 pKa = 5.43LQGVVTFQKK66 pKa = 9.55ATRR69 pKa = 11.84IAALKK74 pKa = 10.11KK75 pKa = 9.69ILPRR79 pKa = 11.84VHH81 pKa = 6.74WEE83 pKa = 3.59IPLNLDD89 pKa = 3.56ASRR92 pKa = 11.84NYY94 pKa = 10.11CMKK97 pKa = 10.53EE98 pKa = 3.18GDD100 pKa = 4.17YY101 pKa = 11.15EE102 pKa = 4.52VTDD105 pKa = 3.58HH106 pKa = 6.59RR107 pKa = 11.84HH108 pKa = 4.36QGRR111 pKa = 11.84RR112 pKa = 11.84SDD114 pKa = 3.14IAEE117 pKa = 4.1AVGMVTDD124 pKa = 5.27GKK126 pKa = 10.51HH127 pKa = 4.25MMEE130 pKa = 4.31VAEE133 pKa = 4.52AHH135 pKa = 6.07PVTYY139 pKa = 10.44VRR141 pKa = 11.84NFRR144 pKa = 11.84GLAALQGILEE154 pKa = 4.47RR155 pKa = 11.84PRR157 pKa = 11.84TWKK160 pKa = 9.14TRR162 pKa = 11.84VHH164 pKa = 5.1VHH166 pKa = 6.45WGPTGTGKK174 pKa = 9.11TYY176 pKa = 10.53NAHH179 pKa = 6.32AQLKK183 pKa = 9.44QLFPLEE189 pKa = 4.29RR190 pKa = 11.84PYY192 pKa = 11.46VWTPAMEE199 pKa = 4.25NWWDD203 pKa = 4.14GYY205 pKa = 10.76SGQSGVILDD214 pKa = 4.01EE215 pKa = 4.88FRR217 pKa = 11.84GQLPFATLLNLLDD230 pKa = 4.57EE231 pKa = 4.96YY232 pKa = 11.63EE233 pKa = 4.46MIVPIKK239 pKa = 10.46GGSRR243 pKa = 11.84QWKK246 pKa = 7.2PHH248 pKa = 5.59VIFITSPLEE257 pKa = 3.73PRR259 pKa = 11.84SWYY262 pKa = 7.74EE263 pKa = 3.4TGKK266 pKa = 10.67AADD269 pKa = 3.64SVEE272 pKa = 3.89QLLRR276 pKa = 11.84RR277 pKa = 11.84IDD279 pKa = 3.93IIEE282 pKa = 4.55HH283 pKa = 4.11MTEE286 pKa = 3.9AYY288 pKa = 9.52KK289 pKa = 10.54ISLDD293 pKa = 5.05DD294 pKa = 3.46IEE296 pKa = 5.04QIYY299 pKa = 9.67KK300 pKa = 10.54

Molecular weight: 34.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL41|A0A0K1RL41_9CIRC Putative capsid protein OS=Didemnum sp. Sea Squirt associated virus OX=1692247 PE=4 SV=1
MM1 pKa = 7.5VFATPFKK8 pKa = 10.59RR9 pKa = 11.84GVKK12 pKa = 10.11RR13 pKa = 11.84LGAHH17 pKa = 6.76AARR20 pKa = 11.84SKK22 pKa = 9.99GVKK25 pKa = 9.24RR26 pKa = 11.84RR27 pKa = 11.84MFGRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PIRR37 pKa = 11.84SRR39 pKa = 11.84FIRR42 pKa = 11.84RR43 pKa = 11.84GRR45 pKa = 11.84NLSSRR50 pKa = 11.84VRR52 pKa = 11.84TLEE55 pKa = 3.88KK56 pKa = 10.1FHH58 pKa = 5.92EE59 pKa = 4.84TKK61 pKa = 10.7FSLSNIAEE69 pKa = 4.06QSPSVGVPTSLNIGSLAVGDD89 pKa = 4.23DD90 pKa = 3.29ANTRR94 pKa = 11.84EE95 pKa = 4.3GNKK98 pKa = 9.76VSLIRR103 pKa = 11.84FDD105 pKa = 5.89LRR107 pKa = 11.84MNWVMADD114 pKa = 3.5AYY116 pKa = 10.01QAIRR120 pKa = 11.84IILVSCDD127 pKa = 3.4HH128 pKa = 6.63VSPADD133 pKa = 3.52LTLANVLDD141 pKa = 4.62ANHH144 pKa = 7.29LIARR148 pKa = 11.84LNINSSYY155 pKa = 11.02KK156 pKa = 9.61RR157 pKa = 11.84DD158 pKa = 3.55QRR160 pKa = 11.84PTPFRR165 pKa = 11.84VIWDD169 pKa = 3.42RR170 pKa = 11.84RR171 pKa = 11.84YY172 pKa = 10.55KK173 pKa = 10.24IRR175 pKa = 11.84SQTFWNTVSEE185 pKa = 4.72GVGVAPDD192 pKa = 3.6DD193 pKa = 3.76SVNIRR198 pKa = 11.84KK199 pKa = 9.43SFHH202 pKa = 6.0FPRR205 pKa = 11.84GRR207 pKa = 11.84KK208 pKa = 6.65MQFRR212 pKa = 11.84TASTDD217 pKa = 3.11NPVRR221 pKa = 11.84GGFKK225 pKa = 10.36LFYY228 pKa = 9.72MSDD231 pKa = 3.4SAAAPHH237 pKa = 5.93PTCLATTQLLFKK249 pKa = 10.9DD250 pKa = 4.28GG251 pKa = 3.69

Molecular weight: 28.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

551

251

300

275.5

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.897 ± 0.69

0.544 ± 0.162

4.719 ± 0.553

5.263 ± 2.105

3.993 ± 1.276

6.352 ± 0.242

3.63 ± 0.797

5.808 ± 0.404

5.808 ± 0.404

7.985 ± 0.524

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.097

4.174 ± 0.647

5.082 ± 0.194

3.448 ± 0.681

8.893 ± 2.481

5.989 ± 1.786

7.26 ± 1.082

6.352 ± 0.527

2.359 ± 0.749

2.904 ± 0.843
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski