Dokdonella fugitiva

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Dokdonella

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2HXH2|A0A4R2HXH2_9GAMM FG-GAP repeat protein OS=Dokdonella fugitiva OX=328517 GN=EV148_11343 PE=4 SV=1
MM1 pKa = 7.29NCISKK6 pKa = 10.82ANAKK10 pKa = 10.01LPSATVLTCAVGVVLAMASSIAHH33 pKa = 6.69AEE35 pKa = 4.16MFCVDD40 pKa = 3.86ATAGLQIALDD50 pKa = 4.08DD51 pKa = 4.53ALSNAEE57 pKa = 3.94PDD59 pKa = 3.91EE60 pKa = 4.24IRR62 pKa = 11.84IQAGDD67 pKa = 3.99YY68 pKa = 10.02ILSAGLVYY76 pKa = 9.6NTQVPGADD84 pKa = 3.11QHH86 pKa = 6.96ALTISGGYY94 pKa = 9.89DD95 pKa = 3.27ADD97 pKa = 3.72CVQRR101 pKa = 11.84IGVSKK106 pKa = 11.04LNGNTQVQVIKK117 pKa = 10.46LVLGAPVFMDD127 pKa = 3.65HH128 pKa = 5.38VTIAGGRR135 pKa = 11.84VEE137 pKa = 4.38NTPGDD142 pKa = 3.79SGRR145 pKa = 11.84GGGVFAQLQGSSAEE159 pKa = 3.96LHH161 pKa = 6.36LDD163 pKa = 3.31AVRR166 pKa = 11.84LISNVASGFGGGIAVISNVGGGSHH190 pKa = 7.2FSLRR194 pKa = 11.84NSLLAGNVAQGAGGAAIGVVSKK216 pKa = 11.05GMVDD220 pKa = 3.23ISNNTITANVSDD232 pKa = 4.22NASAVHH238 pKa = 6.08LQANDD243 pKa = 3.06ASAQFRR249 pKa = 11.84VDD251 pKa = 4.15NNIVWGNGASPSYY264 pKa = 10.49DD265 pKa = 3.52LQLVAPPAGGDD276 pKa = 3.66PYY278 pKa = 11.24HH279 pKa = 6.95LVANDD284 pKa = 3.13IGSTQGMPGDD294 pKa = 4.69DD295 pKa = 3.45SDD297 pKa = 5.72LNVSIDD303 pKa = 3.8PLFVACAFCSNYY315 pKa = 9.33PLSAASPLIDD325 pKa = 4.83AGVGDD330 pKa = 4.33PAGGLAQTDD339 pKa = 3.96LLGAARR345 pKa = 11.84VMGASVDD352 pKa = 3.31MGAYY356 pKa = 9.9EE357 pKa = 4.23FVQPNIAPTVAEE369 pKa = 4.12DD370 pKa = 3.22QAFVVDD376 pKa = 5.03EE377 pKa = 4.53SAAQGAIVGTVTATDD392 pKa = 4.09DD393 pKa = 4.02YY394 pKa = 11.24LPQPHH399 pKa = 7.79AITYY403 pKa = 9.96AIVDD407 pKa = 4.33GNTGDD412 pKa = 4.55AFAIDD417 pKa = 4.17ADD419 pKa = 4.26SGVLTVANPDD429 pKa = 3.94ALDD432 pKa = 3.7STAMPAYY439 pKa = 10.09ALAIKK444 pKa = 9.17VTDD447 pKa = 4.02GEE449 pKa = 4.36LDD451 pKa = 3.45GVGVVNVTVSGPGEE465 pKa = 4.04SDD467 pKa = 4.49VIFQDD472 pKa = 4.23GFDD475 pKa = 3.58GG476 pKa = 4.7

Molecular weight: 47.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2IEM7|A0A4R2IEM7_9GAMM WbqC-like protein OS=Dokdonella fugitiva OX=328517 GN=EV148_10147 PE=4 SV=1
MM1 pKa = 7.06FVAPLSLPSAATRR14 pKa = 11.84AKK16 pKa = 10.18PIAHH20 pKa = 6.4SAWAARR26 pKa = 11.84SPIPWLAGWLLAGLVAVAFIPALRR50 pKa = 11.84GGSTFGATVPFWLVVAPAVDD70 pKa = 4.29LAWLARR76 pKa = 11.84ARR78 pKa = 11.84IGAATSRR85 pKa = 11.84LLRR88 pKa = 11.84RR89 pKa = 11.84MLQRR93 pKa = 11.84TPGQARR99 pKa = 11.84RR100 pKa = 11.84LTVRR104 pKa = 11.84AKK106 pKa = 10.33RR107 pKa = 3.58

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3614

0

3614

1270453

26

5886

351.5

37.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.483 ± 0.066

0.927 ± 0.015

6.182 ± 0.034

5.065 ± 0.043

3.383 ± 0.025

8.797 ± 0.049

2.306 ± 0.022

4.04 ± 0.026

2.377 ± 0.033

10.369 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.836 ± 0.017

2.409 ± 0.031

5.383 ± 0.03

2.979 ± 0.025

8.028 ± 0.06

4.982 ± 0.038

5.096 ± 0.058

7.566 ± 0.03

1.493 ± 0.018

2.301 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski