Flavobacterium sangjuense

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2637 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7PSQ4|A0A4P7PSQ4_9FLAO Uncharacterized protein OS=Flavobacterium sangjuense OX=2518177 GN=GS03_01174 PE=4 SV=1
MM1 pKa = 6.41KK2 pKa = 10.16QKK4 pKa = 10.85YY5 pKa = 9.65ILFILFSLFVFKK17 pKa = 11.17GNAQSNYY24 pKa = 9.39AVNSIPFQPYY34 pKa = 10.05SGTLSPLTTADD45 pKa = 4.52DD46 pKa = 3.87IYY48 pKa = 11.37SPVITMPFSFDD59 pKa = 3.71FYY61 pKa = 11.09GNSYY65 pKa = 7.73TQFVVSTNGYY75 pKa = 8.78VDD77 pKa = 4.41FRR79 pKa = 11.84TSLAGQYY86 pKa = 10.43SPWNVVYY93 pKa = 8.37TIPNAGFGTKK103 pKa = 10.23NSILGCYY110 pKa = 9.37EE111 pKa = 4.03DD112 pKa = 5.46LNNNSTGAIGTITSGVYY129 pKa = 8.75GTAPYY134 pKa = 10.43RR135 pKa = 11.84KK136 pKa = 8.74FVVYY140 pKa = 9.52FNNQPHH146 pKa = 5.74FQCNSTVALSSSQIILSEE164 pKa = 4.02TTNIIDD170 pKa = 3.45VQVINRR176 pKa = 11.84TPCLAWQQGRR186 pKa = 11.84GVIGLVNLDD195 pKa = 3.53GTQAIAPPGRR205 pKa = 11.84NTGNWSANQEE215 pKa = 3.3AWRR218 pKa = 11.84FYY220 pKa = 10.92RR221 pKa = 11.84PGYY224 pKa = 8.04YY225 pKa = 9.43PSYY228 pKa = 11.0SFVRR232 pKa = 11.84CDD234 pKa = 3.84DD235 pKa = 4.64DD236 pKa = 4.98SDD238 pKa = 4.57SFVTFDD244 pKa = 3.85LTVAANDD251 pKa = 4.33LSPANPSAISFFEE264 pKa = 4.87DD265 pKa = 3.03NALTIPVANPTAYY278 pKa = 9.7INTSNPRR285 pKa = 11.84TVYY288 pKa = 10.97ASGNGAIRR296 pKa = 11.84SVILSVIDD304 pKa = 3.79CSIDD308 pKa = 3.46ADD310 pKa = 4.01SDD312 pKa = 4.31SVPSATEE319 pKa = 3.77DD320 pKa = 3.57VNNDD324 pKa = 2.97TNLANDD330 pKa = 3.97DD331 pKa = 4.11TDD333 pKa = 5.49LDD335 pKa = 4.78GIPNYY340 pKa = 10.49LDD342 pKa = 4.06NDD344 pKa = 4.18DD345 pKa = 6.02DD346 pKa = 5.88GDD348 pKa = 4.08LVLTSVEE355 pKa = 4.29YY356 pKa = 11.04VFARR360 pKa = 11.84VNPRR364 pKa = 11.84QVNVILDD371 pKa = 3.74TDD373 pKa = 4.19LDD375 pKa = 4.73GIPNYY380 pKa = 10.49LDD382 pKa = 4.14NDD384 pKa = 4.21DD385 pKa = 5.81DD386 pKa = 6.21GDD388 pKa = 4.45GLLTWRR394 pKa = 11.84EE395 pKa = 4.24DD396 pKa = 3.71YY397 pKa = 11.44NHH399 pKa = 7.52DD400 pKa = 4.15GNPGNDD406 pKa = 3.69DD407 pKa = 3.84TNSSGTADD415 pKa = 3.64YY416 pKa = 11.16LEE418 pKa = 4.76SSVALGVTPVSIDD431 pKa = 3.36NNSIKK436 pKa = 10.41VFPNPATNVLNIQNNTDD453 pKa = 3.51DD454 pKa = 4.01TNASIEE460 pKa = 4.12IYY462 pKa = 10.05SISGAKK468 pKa = 9.52VKK470 pKa = 10.42SLKK473 pKa = 7.82TTQALTTIAVSDD485 pKa = 4.16LQSGVYY491 pKa = 8.67FVKK494 pKa = 9.72VTMNNQVGNYY504 pKa = 9.79KK505 pKa = 10.46FIKK508 pKa = 9.93NN509 pKa = 3.51

Molecular weight: 55.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7PS10|A0A4P7PS10_9FLAO Response regulatory domain-containing protein OS=Flavobacterium sangjuense OX=2518177 GN=GS03_01127 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.35KK16 pKa = 10.31RR17 pKa = 11.84KK18 pKa = 9.82KK19 pKa = 8.65RR20 pKa = 11.84ARR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.91KK27 pKa = 10.59KK28 pKa = 9.82KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2637

0

2637

965529

29

4907

366.1

40.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.888 ± 0.065

0.909 ± 0.03

5.289 ± 0.045

5.889 ± 0.087

5.233 ± 0.047

6.66 ± 0.072

1.601 ± 0.028

7.838 ± 0.053

7.431 ± 0.11

8.716 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.034

6.407 ± 0.049

3.542 ± 0.042

3.317 ± 0.026

2.965 ± 0.041

6.689 ± 0.06

6.952 ± 0.165

6.49 ± 0.039

1.02 ± 0.015

4.035 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski