Emiliania huxleyi virus 86 (isolate United Kingdom/English Channel/1999) (EhV-86)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Coccolithovirus; Emiliania huxleyi virus 86

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4A372|Q4A372_EHV8U Sphingolipid 4-desaturase OS=Emiliania huxleyi virus 86 (isolate United Kingdom/English Channel/1999) OX=654925 GN=EhV061 PE=3 SV=1
MM1 pKa = 7.24SVCKK5 pKa = 9.37ITNDD9 pKa = 5.27GIDD12 pKa = 3.35LANNVDD18 pKa = 3.48VDD20 pKa = 3.81TLYY23 pKa = 10.92KK24 pKa = 10.23RR25 pKa = 11.84EE26 pKa = 4.05EE27 pKa = 4.27VVIQPPASSLRR38 pKa = 11.84ICLYY42 pKa = 10.46TLDD45 pKa = 4.67KK46 pKa = 10.91PGTPVKK52 pKa = 10.56QSTLLSYY59 pKa = 10.74DD60 pKa = 3.94LPPPFDD66 pKa = 3.56NYY68 pKa = 11.01LYY70 pKa = 10.48NSPVYY75 pKa = 9.16MQFIGGTITSVSQATQLIKK94 pKa = 10.89LLGEE98 pKa = 4.36IFVHH102 pKa = 6.39TDD104 pKa = 3.19DD105 pKa = 5.45SVSDD109 pKa = 4.12SNTLVYY115 pKa = 10.62EE116 pKa = 4.0KK117 pKa = 11.17VEE119 pKa = 4.08DD120 pKa = 3.9VEE122 pKa = 4.77SEE124 pKa = 4.13EE125 pKa = 4.5EE126 pKa = 4.23EE127 pKa = 4.12EE128 pKa = 4.62DD129 pKa = 4.25DD130 pKa = 5.39VPEE133 pKa = 4.38EE134 pKa = 4.38YY135 pKa = 10.56YY136 pKa = 11.14DD137 pKa = 3.68HH138 pKa = 7.26NEE140 pKa = 3.67GSEE143 pKa = 3.99YY144 pKa = 11.02EE145 pKa = 4.05EE146 pKa = 5.84DD147 pKa = 3.54EE148 pKa = 4.33DD149 pKa = 4.59VAYY152 pKa = 10.95SDD154 pKa = 6.17DD155 pKa = 4.07EE156 pKa = 6.76DD157 pKa = 5.97DD158 pKa = 5.8DD159 pKa = 4.32EE160 pKa = 6.81SKK162 pKa = 11.31VEE164 pKa = 4.4DD165 pKa = 3.66NSTVYY170 pKa = 10.89EE171 pKa = 4.63DD172 pKa = 5.38DD173 pKa = 4.39SSLKK177 pKa = 10.73

Molecular weight: 20.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4A2V5|Q4A2V5_EHV8U Major Facilitator Superfamily protein OS=Emiliania huxleyi virus 86 (isolate United Kingdom/English Channel/1999) OX=654925 GN=EhV179 PE=4 SV=1
MM1 pKa = 7.25AVSRR5 pKa = 11.84RR6 pKa = 11.84SRR8 pKa = 11.84YY9 pKa = 7.36GTVRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TVRR20 pKa = 11.84KK21 pKa = 8.12STASRR26 pKa = 11.84STKK29 pKa = 9.49VGRR32 pKa = 11.84VAKK35 pKa = 9.99LSNMSAGCRR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 10.55VKK48 pKa = 10.54SQLGNRR54 pKa = 11.84KK55 pKa = 9.43LSRR58 pKa = 11.84ATRR61 pKa = 11.84ASIIRR66 pKa = 11.84RR67 pKa = 11.84SVKK70 pKa = 8.22TWSRR74 pKa = 11.84MSKK77 pKa = 9.88ARR79 pKa = 11.84KK80 pKa = 8.16SRR82 pKa = 11.84YY83 pKa = 5.01MTRR86 pKa = 11.84KK87 pKa = 8.7SSKK90 pKa = 10.01SKK92 pKa = 10.52RR93 pKa = 11.84KK94 pKa = 4.15TTRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84TKK101 pKa = 7.71RR102 pKa = 11.84TKK104 pKa = 9.69RR105 pKa = 11.84RR106 pKa = 11.84SSRR109 pKa = 11.84RR110 pKa = 11.84TKK112 pKa = 9.97RR113 pKa = 11.84RR114 pKa = 11.84SSRR117 pKa = 11.84RR118 pKa = 11.84STMKK122 pKa = 10.35RR123 pKa = 11.84PKK125 pKa = 9.54TSTMSKK131 pKa = 10.35GLKK134 pKa = 9.86KK135 pKa = 10.63YY136 pKa = 10.42IRR138 pKa = 11.84TKK140 pKa = 10.44SGKK143 pKa = 9.72KK144 pKa = 9.61NLSKK148 pKa = 11.04VSTKK152 pKa = 7.7MLRR155 pKa = 11.84KK156 pKa = 9.62LVALWRR162 pKa = 11.84RR163 pKa = 11.84MSAKK167 pKa = 10.19SKK169 pKa = 10.43AKK171 pKa = 9.76YY172 pKa = 9.51SVRR175 pKa = 11.84STRR178 pKa = 11.84KK179 pKa = 7.96RR180 pKa = 11.84STRR183 pKa = 11.84KK184 pKa = 8.83RR185 pKa = 11.84STRR188 pKa = 11.84KK189 pKa = 8.83RR190 pKa = 11.84STRR193 pKa = 11.84KK194 pKa = 8.83RR195 pKa = 11.84STRR198 pKa = 11.84KK199 pKa = 8.83RR200 pKa = 11.84STRR203 pKa = 11.84KK204 pKa = 8.83RR205 pKa = 11.84STRR208 pKa = 11.84KK209 pKa = 8.83RR210 pKa = 11.84STRR213 pKa = 11.84KK214 pKa = 8.83RR215 pKa = 11.84STRR218 pKa = 11.84KK219 pKa = 8.83RR220 pKa = 11.84STRR223 pKa = 11.84RR224 pKa = 11.84RR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84TTRR230 pKa = 11.84KK231 pKa = 9.12RR232 pKa = 11.84RR233 pKa = 11.84RR234 pKa = 11.84SRR236 pKa = 11.84PRR238 pKa = 11.84TRR240 pKa = 11.84RR241 pKa = 11.84VRR243 pKa = 11.84RR244 pKa = 11.84TRR246 pKa = 11.84RR247 pKa = 11.84TRR249 pKa = 11.84RR250 pKa = 11.84TVRR253 pKa = 11.84RR254 pKa = 11.84RR255 pKa = 3.12

Molecular weight: 31.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

472

0

472

123003

20

2873

260.6

29.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.123

1.829 ± 0.075

5.716 ± 0.092

5.094 ± 0.117

3.833 ± 0.083

5.254 ± 0.128

2.532 ± 0.082

7.259 ± 0.138

5.768 ± 0.204

7.462 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.069 ± 0.092

5.553 ± 0.099

6.481 ± 0.718

3.018 ± 0.07

4.704 ± 0.119

7.493 ± 0.166

6.806 ± 0.095

6.416 ± 0.109

1.092 ± 0.041

4.228 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski