Bacillus phage BCP78

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Tsarbombavirus; Bacillus virus BCP78

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9J269|G9J269_9CAUD Uncharacterized protein OS=Bacillus phage BCP78 OX=1126950 GN=BCP78_0042 PE=4 SV=1
MM1 pKa = 7.39TKK3 pKa = 10.37LISVYY8 pKa = 10.68YY9 pKa = 9.9DD10 pKa = 3.06NNGINIYY17 pKa = 10.61LDD19 pKa = 3.18AVVTNEE25 pKa = 4.2GEE27 pKa = 4.16WARR30 pKa = 11.84VEE32 pKa = 3.52QDD34 pKa = 4.21LYY36 pKa = 10.88IEE38 pKa = 4.73HH39 pKa = 7.7DD40 pKa = 3.24NGFRR44 pKa = 11.84VEE46 pKa = 4.12QFHH49 pKa = 7.68DD50 pKa = 3.81VLGLCNLPNNTNLVIDD66 pKa = 4.22NQDD69 pKa = 3.69NICIIMQEE77 pKa = 4.18LFEE80 pKa = 4.82EE81 pKa = 4.49DD82 pKa = 3.56HH83 pKa = 6.51NN84 pKa = 4.41

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9J249|G9J249_9CAUD Thymidylate synthase OS=Bacillus phage BCP78 OX=1126950 GN=BCP78_0022 PE=3 SV=1
MM1 pKa = 7.29SRR3 pKa = 11.84NARR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84FLKK12 pKa = 10.71NNQATKK18 pKa = 10.8SAMQNVGNALVQGTLDD34 pKa = 3.22AGMKK38 pKa = 9.62EE39 pKa = 4.9VIRR42 pKa = 11.84QQPKK46 pKa = 10.2DD47 pKa = 3.26ISATRR52 pKa = 11.84MPKK55 pKa = 9.68YY56 pKa = 10.62LKK58 pKa = 9.55LTSEE62 pKa = 4.29RR63 pKa = 11.84LEE65 pKa = 3.99KK66 pKa = 11.24LEE68 pKa = 5.06VIDD71 pKa = 4.75LKK73 pKa = 11.21PFFAHH78 pKa = 5.76SSKK81 pKa = 10.53RR82 pKa = 11.84KK83 pKa = 8.69KK84 pKa = 10.76SKK86 pKa = 10.81DD87 pKa = 3.06GGWYY91 pKa = 10.03LRR93 pKa = 11.84VPIKK97 pKa = 10.59RR98 pKa = 11.84KK99 pKa = 9.84ARR101 pKa = 11.84DD102 pKa = 3.26MSRR105 pKa = 11.84RR106 pKa = 11.84MYY108 pKa = 9.88EE109 pKa = 3.47QLRR112 pKa = 11.84AIDD115 pKa = 3.67IAPSEE120 pKa = 4.3RR121 pKa = 11.84QTVISDD127 pKa = 3.26YY128 pKa = 10.98LYY130 pKa = 10.92DD131 pKa = 3.83RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84DD135 pKa = 3.28SDD137 pKa = 4.0ASMLNYY143 pKa = 9.83EE144 pKa = 4.78PKK146 pKa = 10.42SKK148 pKa = 10.8NITKK152 pKa = 9.77IRR154 pKa = 11.84SGNNRR159 pKa = 11.84HH160 pKa = 6.77DD161 pKa = 3.62YY162 pKa = 10.15VAFRR166 pKa = 11.84TVSNKK171 pKa = 9.82SPQSSWIINRR181 pKa = 11.84GKK183 pKa = 11.01VNTQDD188 pKa = 3.06TSKK191 pKa = 9.85TFVANVNRR199 pKa = 11.84LMKK202 pKa = 10.03WKK204 pKa = 9.24MKK206 pKa = 10.75NGLL209 pKa = 3.63

Molecular weight: 24.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

46626

43

1299

205.4

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.612 ± 0.204

0.721 ± 0.063

5.894 ± 0.127

7.764 ± 0.297

3.991 ± 0.125

6.453 ± 0.28

1.812 ± 0.111

6.488 ± 0.144

8.045 ± 0.228

8.021 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.108

5.347 ± 0.164

3.457 ± 0.178

3.822 ± 0.133

4.429 ± 0.145

5.72 ± 0.228

6.179 ± 0.242

7.007 ± 0.165

1.16 ± 0.073

4.309 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski