Oceanobacillus picturae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9AJY8|W9AJY8_9BACI Glutathione transport system permease protein GsiD OS=Oceanobacillus picturae OX=171693 GN=gsiD_2 PE=3 SV=1
MM1 pKa = 7.18HH2 pKa = 7.52LEE4 pKa = 3.71WFDD7 pKa = 3.85RR8 pKa = 11.84VCEE11 pKa = 3.92EE12 pKa = 4.4LQEE15 pKa = 4.33SLNSICEE22 pKa = 4.15KK23 pKa = 10.06YY24 pKa = 10.63DD25 pKa = 3.29EE26 pKa = 4.5NGRR29 pKa = 11.84MSIEE33 pKa = 4.1RR34 pKa = 11.84GAKK37 pKa = 9.54HH38 pKa = 6.05PRR40 pKa = 11.84LDD42 pKa = 3.19FFTYY46 pKa = 10.24SGEE49 pKa = 3.7EE50 pKa = 4.28DD51 pKa = 2.87IDD53 pKa = 4.1YY54 pKa = 10.81FCTLFFDD61 pKa = 5.0PYY63 pKa = 10.98NEE65 pKa = 3.81EE66 pKa = 5.15FYY68 pKa = 10.75IQTIDD73 pKa = 3.53PEE75 pKa = 4.34YY76 pKa = 10.63GLTSKK81 pKa = 10.73VVLEE85 pKa = 5.42DD86 pKa = 3.59IDD88 pKa = 6.41DD89 pKa = 3.82IMDD92 pKa = 4.0AVHH95 pKa = 6.82EE96 pKa = 4.56SFHH99 pKa = 6.77EE100 pKa = 4.26YY101 pKa = 10.26IEE103 pKa = 4.84ADD105 pKa = 4.08FIEE108 pKa = 4.91TEE110 pKa = 4.01DD111 pKa = 3.6SGYY114 pKa = 10.74IMDD117 pKa = 5.58DD118 pKa = 3.45EE119 pKa = 4.86EE120 pKa = 4.52EE121 pKa = 3.86TDD123 pKa = 3.48EE124 pKa = 5.61IYY126 pKa = 10.85FEE128 pKa = 4.86EE129 pKa = 5.91IDD131 pKa = 4.28VEE133 pKa = 4.36WEE135 pKa = 4.22TPEE138 pKa = 3.57VTAYY142 pKa = 10.67YY143 pKa = 10.68DD144 pKa = 3.58EE145 pKa = 5.84DD146 pKa = 3.8EE147 pKa = 4.91VEE149 pKa = 3.64ISYY152 pKa = 10.67QFGVVGEE159 pKa = 4.41TGDD162 pKa = 3.9GVLKK166 pKa = 9.11RR167 pKa = 11.84TNRR170 pKa = 11.84IWTKK174 pKa = 11.29DD175 pKa = 3.09DD176 pKa = 4.93DD177 pKa = 4.73LIKK180 pKa = 10.93DD181 pKa = 3.46EE182 pKa = 4.52SHH184 pKa = 7.42FIFSKK189 pKa = 11.13DD190 pKa = 3.29EE191 pKa = 4.08ASTIIAMIASHH202 pKa = 6.62MDD204 pKa = 2.91SLAPLEE210 pKa = 4.35EE211 pKa = 5.42GEE213 pKa = 4.44VV214 pKa = 3.48

Molecular weight: 25.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9AHD8|W9AHD8_9BACI Metal-dependent carboxypeptidase OS=Oceanobacillus picturae OX=171693 GN=ypwA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3656

0

3656

1034527

29

1537

283.0

31.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.038 ± 0.042

0.602 ± 0.01

5.311 ± 0.034

7.867 ± 0.052

4.475 ± 0.034

6.83 ± 0.037

2.054 ± 0.019

7.748 ± 0.043

6.703 ± 0.035

9.746 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.836 ± 0.019

4.422 ± 0.028

3.517 ± 0.022

3.826 ± 0.028

3.897 ± 0.034

5.985 ± 0.029

5.579 ± 0.029

6.999 ± 0.031

1.019 ± 0.013

3.544 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski