St Croix River virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q9DSP3|Q9DSP3_9REOV NS2 OS=St Croix River virus OX=104581 PE=4 SV=1
MM1 pKa = 7.6SDD3 pKa = 2.72SRR5 pKa = 11.84AYY7 pKa = 10.33AIEE10 pKa = 4.07ILDD13 pKa = 4.04LFADD17 pKa = 3.92QVPPLPGGDD26 pKa = 3.32SRR28 pKa = 11.84ALSRR32 pKa = 11.84ARR34 pKa = 11.84AFLRR38 pKa = 11.84NPIVVRR44 pKa = 11.84NYY46 pKa = 10.54PNLDD50 pKa = 3.44PLGNDD55 pKa = 2.66ATVRR59 pKa = 11.84RR60 pKa = 11.84NLFFVCLDD68 pKa = 3.47VAMLALDD75 pKa = 3.26INEE78 pKa = 3.91FKK80 pKa = 11.15VRR82 pKa = 11.84IEE84 pKa = 3.76SHH86 pKa = 5.25RR87 pKa = 11.84VARR90 pKa = 11.84EE91 pKa = 3.49LAALLGPTGADD102 pKa = 3.43PQPTAVQIAEE112 pKa = 4.19SFTDD116 pKa = 3.29KK117 pKa = 11.15GRR119 pKa = 11.84TTYY122 pKa = 11.18AVGQDD127 pKa = 3.22GPVYY131 pKa = 10.49IEE133 pKa = 4.33GAYY136 pKa = 10.36SPSPFTYY143 pKa = 9.97GPRR146 pKa = 11.84YY147 pKa = 8.6VQDD150 pKa = 3.49GNFVNVVGPVRR161 pKa = 11.84DD162 pKa = 3.74GAAPPEE168 pKa = 4.28PCFLVANSPGRR179 pKa = 11.84VTVILNGGPNSLALEE194 pKa = 4.61HH195 pKa = 6.81IFRR198 pKa = 11.84TILRR202 pKa = 11.84RR203 pKa = 11.84GSLFTARR210 pKa = 11.84WYY212 pKa = 10.92ANSCFIGEE220 pKa = 4.15NGAPIITNGVPSLTSGGGGTNIPAYY245 pKa = 10.58FPFTFSKK252 pKa = 10.64RR253 pKa = 11.84STFTARR259 pKa = 11.84VRR261 pKa = 11.84GGGAWPVAAITIEE274 pKa = 3.93FLSFYY279 pKa = 10.55SIGHH283 pKa = 6.48FGDD286 pKa = 4.82PYY288 pKa = 10.76PGFSTDD294 pKa = 3.37LQDD297 pKa = 4.89FYY299 pKa = 11.11TYY301 pKa = 10.64TNPVWHH307 pKa = 7.62ALRR310 pKa = 11.84KK311 pKa = 8.86QLSSAMDD318 pKa = 3.96LSMYY322 pKa = 10.51VATKK326 pKa = 9.95YY327 pKa = 11.23DD328 pKa = 3.73DD329 pKa = 3.79FTRR332 pKa = 11.84HH333 pKa = 5.59QMLALALFGRR343 pKa = 11.84LSRR346 pKa = 11.84IFRR349 pKa = 11.84RR350 pKa = 11.84LDD352 pKa = 3.24PDD354 pKa = 4.28LRR356 pKa = 11.84VLPLDD361 pKa = 4.11NGDD364 pKa = 4.36GEE366 pKa = 4.63AEE368 pKa = 4.1AYY370 pKa = 9.86AAEE373 pKa = 4.33AAVEE377 pKa = 4.17EE378 pKa = 4.57AA379 pKa = 4.7

Molecular weight:
41.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9DSP1|Q9DSP1_9REOV VP6 OS=St Croix River virus OX=104581 PE=4 SV=1
MM1 pKa = 7.31QKK3 pKa = 10.03QSHH6 pKa = 5.82LTMMPRR12 pKa = 11.84PTAVVMTKK20 pKa = 10.64DD21 pKa = 3.38GIGAEE26 pKa = 4.36RR27 pKa = 11.84VTLMPSAPSVSPLQVISEE45 pKa = 4.41TLSVPSAKK53 pKa = 9.66TPPEE57 pKa = 4.0RR58 pKa = 11.84MEE60 pKa = 3.89KK61 pKa = 10.44AALKK65 pKa = 10.0GAQEE69 pKa = 4.91AISDD73 pKa = 3.86SSDD76 pKa = 3.89PIGMLIKK83 pKa = 10.63VEE85 pKa = 4.59ANASTISALQHH96 pKa = 5.67GEE98 pKa = 3.82RR99 pKa = 11.84KK100 pKa = 9.1LARR103 pKa = 11.84RR104 pKa = 11.84KK105 pKa = 10.15SLLFWLHH112 pKa = 5.73LTTVILMLVFTAISSIANLSIHH134 pKa = 6.53LEE136 pKa = 4.09HH137 pKa = 6.9YY138 pKa = 10.94LRR140 pKa = 11.84MANLSSNMICLGFATSTMFITRR162 pKa = 11.84TRR164 pKa = 11.84LSVALEE170 pKa = 3.64LHH172 pKa = 6.19KK173 pKa = 10.83VRR175 pKa = 11.84KK176 pKa = 7.87QLRR179 pKa = 11.84KK180 pKa = 9.45RR181 pKa = 11.84LAYY184 pKa = 9.89QATAKK189 pKa = 10.52NISAARR195 pKa = 11.84APPPSQPFLSDD206 pKa = 3.31VSSGTSGWIVAPGCKK221 pKa = 9.72ALQQ224 pKa = 3.55

Molecular weight:
24.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5863

224

1345

586.3

65.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.846 ± 0.771

0.955 ± 0.151

5.611 ± 0.381

5.151 ± 0.41

4.844 ± 0.364

5.987 ± 0.251

3.087 ± 0.271

5.373 ± 0.35

3.275 ± 0.318

10.387 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.193

2.729 ± 0.247

6.55 ± 0.448

3.377 ± 0.297

7.266 ± 0.315

9.006 ± 0.442

5.526 ± 0.448

6.669 ± 0.24

0.972 ± 0.222

3.036 ± 0.2

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski