Hubei tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH12|A0A1L3KH12_9VIRU Capsid protein OS=Hubei tombus-like virus 2 OX=1923266 PE=3 SV=1
MM1 pKa = 7.27PTSNYY6 pKa = 10.24DD7 pKa = 3.21VLGIGGSTTLRR18 pKa = 11.84AADD21 pKa = 3.68GTQLISYY28 pKa = 9.43DD29 pKa = 3.75PGSAGSIMVNVSGKK43 pKa = 9.59PLFVDD48 pKa = 4.92FTAFFPDD55 pKa = 3.44VRR57 pKa = 11.84KK58 pKa = 10.5SGIGRR63 pKa = 11.84PSLKK67 pKa = 9.25TRR69 pKa = 11.84HH70 pKa = 6.17NLTEE74 pKa = 4.23SYY76 pKa = 8.39QTVVDD81 pKa = 4.55EE82 pKa = 4.57NADD85 pKa = 3.43LSRR88 pKa = 11.84FGGEE92 pKa = 3.63PKK94 pKa = 10.22KK95 pKa = 10.78GYY97 pKa = 9.9EE98 pKa = 4.06YY99 pKa = 9.94STSRR103 pKa = 11.84TIQPDD108 pKa = 4.25GYY110 pKa = 10.89FILQWADD117 pKa = 3.47LNDD120 pKa = 4.05SRR122 pKa = 11.84TMPQTRR128 pKa = 11.84VKK130 pKa = 10.85VVISDD135 pKa = 3.72TLGEE139 pKa = 4.32RR140 pKa = 11.84GPVVTGGSRR149 pKa = 11.84DD150 pKa = 3.71TVDD153 pKa = 3.14GKK155 pKa = 9.83GTEE158 pKa = 4.38VIAITRR164 pKa = 11.84QPTVGASFTSWAGSYY179 pKa = 9.95TYY181 pKa = 10.47TDD183 pKa = 3.59PLGFGANINRR193 pKa = 11.84LPSFITATRR202 pKa = 11.84YY203 pKa = 9.99RR204 pKa = 11.84LGGSMDD210 pKa = 3.61SVALRR215 pKa = 11.84NDD217 pKa = 2.8SDD219 pKa = 3.86MEE221 pKa = 4.18YY222 pKa = 10.41VICFSLVINNFQGSIRR238 pKa = 11.84YY239 pKa = 7.43YY240 pKa = 10.08CPNGEE245 pKa = 4.06RR246 pKa = 11.84TEE248 pKa = 4.09MEE250 pKa = 4.21LNGRR254 pKa = 11.84TIGVATVAFPPGGIMAYY271 pKa = 10.18GYY273 pKa = 10.19DD274 pKa = 3.86AYY276 pKa = 11.01SDD278 pKa = 3.43TGFPPVAVTMSVVPGKK294 pKa = 10.77YY295 pKa = 9.58SDD297 pKa = 3.65VFTTGISVLL306 pKa = 3.8

Molecular weight: 33.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH12|A0A1L3KH12_9VIRU Capsid protein OS=Hubei tombus-like virus 2 OX=1923266 PE=3 SV=1
MM1 pKa = 7.69PPKK4 pKa = 10.42NNQVSKK10 pKa = 10.94RR11 pKa = 11.84LAARR15 pKa = 11.84KK16 pKa = 9.29APRR19 pKa = 11.84PKK21 pKa = 9.98TSSSGNRR28 pKa = 11.84LVTAPQSRR36 pKa = 11.84AVRR39 pKa = 11.84ATVPSGDD46 pKa = 3.36RR47 pKa = 11.84KK48 pKa = 10.13QICVMTIKK56 pKa = 10.52RR57 pKa = 11.84PAGLTEE63 pKa = 4.01WAQEE67 pKa = 4.22YY68 pKa = 8.5TLHH71 pKa = 7.43PDD73 pKa = 4.07NIPWLEE79 pKa = 4.47GIAPSYY85 pKa = 9.46QRR87 pKa = 11.84WGLKK91 pKa = 8.79GLKK94 pKa = 8.82VWYY97 pKa = 9.13EE98 pKa = 3.84PRR100 pKa = 11.84ATALTPGTVSMAILSDD116 pKa = 4.13FKK118 pKa = 11.39DD119 pKa = 3.73GTPKK123 pKa = 10.55SLQSLTSVKK132 pKa = 10.26GAVRR136 pKa = 11.84GAPWDD141 pKa = 3.94KK142 pKa = 10.27FTLSCPKK149 pKa = 9.75FRR151 pKa = 11.84TYY153 pKa = 10.87EE154 pKa = 4.01YY155 pKa = 10.57VSDD158 pKa = 3.93VASLSGEE165 pKa = 4.05DD166 pKa = 3.77LNNRR170 pKa = 11.84ALGKK174 pKa = 10.13IVVCADD180 pKa = 3.15MDD182 pKa = 4.08DD183 pKa = 4.09SFTVGSIVGRR193 pKa = 11.84IFIEE197 pKa = 4.21YY198 pKa = 10.43SDD200 pKa = 4.38VLLDD204 pKa = 5.02SIDD207 pKa = 3.41PTLQRR212 pKa = 11.84KK213 pKa = 8.14SAATSPGTT221 pKa = 3.53

Molecular weight: 24.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1322

221

570

330.5

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.413 ± 0.922

1.815 ± 0.709

5.673 ± 0.319

5.9 ± 1.224

3.782 ± 0.647

8.245 ± 0.912

1.664 ± 0.586

4.085 ± 0.482

4.841 ± 0.765

7.186 ± 0.64

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.175

3.631 ± 0.244

5.522 ± 0.63

2.799 ± 0.214

7.64 ± 0.881

7.413 ± 0.956

6.203 ± 1.222

7.943 ± 0.667

2.118 ± 0.64

3.707 ± 0.642
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski