Clostridium algidicarnis DSM 15099

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium algidicarnis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S6FVT8|A0A2S6FVT8_9CLOT Lipopolysaccharide cholinephosphotransferase OS=Clostridium algidicarnis DSM 15099 OX=1121295 GN=BD821_11646 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.38ISKK5 pKa = 10.46EE6 pKa = 3.88MTIGDD11 pKa = 3.69VVMEE15 pKa = 4.18YY16 pKa = 10.3PEE18 pKa = 4.26SVEE21 pKa = 3.9VLMSFGMGCVGCPASQMEE39 pKa = 4.51TLEE42 pKa = 4.13EE43 pKa = 3.64AAMVHH48 pKa = 6.13GFDD51 pKa = 4.57IKK53 pKa = 11.06EE54 pKa = 4.16LLSALNGG61 pKa = 3.67

Molecular weight:
6.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S6G1E5|A0A2S6G1E5_9CLOT Cell division protein FtsI/penicillin-binding protein 2 OS=Clostridium algidicarnis DSM 15099 OX=1121295 GN=BD821_101283 PE=4 SV=1
MM1 pKa = 7.53SRR3 pKa = 11.84RR4 pKa = 11.84CEE6 pKa = 4.0YY7 pKa = 10.33CDD9 pKa = 3.4KK10 pKa = 11.28GVMSGVQYY18 pKa = 10.81SHH20 pKa = 6.33SHH22 pKa = 5.36RR23 pKa = 11.84QSKK26 pKa = 10.2RR27 pKa = 11.84KK28 pKa = 7.68WAPNIKK34 pKa = 9.68KK35 pKa = 9.98IKK37 pKa = 10.06AIVNGTPRR45 pKa = 11.84TVHH48 pKa = 4.66VCTRR52 pKa = 11.84CLRR55 pKa = 11.84SGKK58 pKa = 7.94VEE60 pKa = 3.89RR61 pKa = 11.84AII63 pKa = 4.51

Molecular weight:
7.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2618

0

2618

808886

29

2948

309.0

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.383 ± 0.047

1.063 ± 0.018

5.758 ± 0.035

7.606 ± 0.058

4.261 ± 0.035

6.441 ± 0.045

1.331 ± 0.017

10.192 ± 0.055

9.419 ± 0.048

9.129 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.734 ± 0.02

6.033 ± 0.038

2.832 ± 0.025

2.15 ± 0.024

3.33 ± 0.033

6.569 ± 0.039

4.754 ± 0.032

6.351 ± 0.039

0.606 ± 0.016

4.057 ± 0.034

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski