Cryobacterium roopkundense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099J456|A0A099J456_9MICO Enoyl-CoA hydratase OS=Cryobacterium roopkundense OX=1001240 GN=BJ997_003394 PE=3 SV=1
MM1 pKa = 6.3EE2 pKa = 4.04TRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84GLMATATVVASALFLTGCVQSDD28 pKa = 3.82RR29 pKa = 11.84DD30 pKa = 3.61DD31 pKa = 3.68TAAGDD36 pKa = 3.6VDD38 pKa = 4.11STFVFAASSDD48 pKa = 3.91PASLDD53 pKa = 3.26PAFASDD59 pKa = 3.96GEE61 pKa = 4.44SFRR64 pKa = 11.84VSRR67 pKa = 11.84QIFEE71 pKa = 4.37GLVGVAPGTADD82 pKa = 3.85PAPLLAEE89 pKa = 4.38SWDD92 pKa = 3.7QSDD95 pKa = 5.27DD96 pKa = 3.56GLSYY100 pKa = 10.85TFALKK105 pKa = 10.63EE106 pKa = 3.95GVTFHH111 pKa = 7.79DD112 pKa = 4.21GTDD115 pKa = 3.81FDD117 pKa = 5.35AEE119 pKa = 4.15AVCFNFDD126 pKa = 2.43RR127 pKa = 11.84WFNFTGIAQSPSVAYY142 pKa = 10.45YY143 pKa = 10.03YY144 pKa = 11.51GNLFRR149 pKa = 11.84AYY151 pKa = 10.35ADD153 pKa = 3.55NPEE156 pKa = 3.95GAVYY160 pKa = 10.12DD161 pKa = 3.91SCSTGDD167 pKa = 3.37TGEE170 pKa = 4.23VTVTLAQPFAGFIAALSLPAFSMQSPTALEE200 pKa = 3.92EE201 pKa = 3.97FAADD205 pKa = 4.32DD206 pKa = 3.45IGGSEE211 pKa = 4.22EE212 pKa = 4.21APALSEE218 pKa = 4.12YY219 pKa = 10.93AQGHH223 pKa = 4.88PTGTGPFAFDD233 pKa = 2.71SWEE236 pKa = 3.89IGSQLEE242 pKa = 3.79LSAYY246 pKa = 9.35SDD248 pKa = 3.65YY249 pKa = 10.74WGEE252 pKa = 3.99QGDD255 pKa = 3.9VQSIIFRR262 pKa = 11.84VIDD265 pKa = 3.94DD266 pKa = 3.78PQARR270 pKa = 11.84RR271 pKa = 11.84QSLEE275 pKa = 3.63AGSIDD280 pKa = 4.58GYY282 pKa = 11.3DD283 pKa = 3.52LVAPADD289 pKa = 4.0TVALEE294 pKa = 4.08TAGFQIVPRR303 pKa = 11.84DD304 pKa = 3.82PFTILYY310 pKa = 10.21LGINQSVPEE319 pKa = 4.17LQDD322 pKa = 3.02VRR324 pKa = 11.84VRR326 pKa = 11.84EE327 pKa = 4.63AISLAIDD334 pKa = 3.32KK335 pKa = 10.25QQLIDD340 pKa = 3.4QTLPEE345 pKa = 4.57GTQLATQFIPPVVAGYY361 pKa = 10.82NDD363 pKa = 4.6DD364 pKa = 3.77VTTYY368 pKa = 10.93DD369 pKa = 3.85YY370 pKa = 11.27DD371 pKa = 3.8QEE373 pKa = 4.2AAKK376 pKa = 10.46QLLADD381 pKa = 4.33AGYY384 pKa = 9.72PDD386 pKa = 5.41GFTLQFNYY394 pKa = 7.61PTGVSRR400 pKa = 11.84PYY402 pKa = 9.69MPTPEE407 pKa = 4.08EE408 pKa = 4.13VFTNISAQLGAIGITIDD425 pKa = 3.76PQPNAWSPDD434 pKa = 3.44YY435 pKa = 10.94LDD437 pKa = 5.69RR438 pKa = 11.84IQGSEE443 pKa = 3.51NHH445 pKa = 6.86GIHH448 pKa = 6.53LLGWTGDD455 pKa = 3.85YY456 pKa = 11.17NDD458 pKa = 3.83TDD460 pKa = 3.59NFVGVFFGTSSLEE473 pKa = 3.94FGFDD477 pKa = 3.43NPEE480 pKa = 3.76LFAALTEE487 pKa = 4.04ARR489 pKa = 11.84GISSLEE495 pKa = 3.95EE496 pKa = 3.65QVPMYY501 pKa = 10.34EE502 pKa = 4.69VINEE506 pKa = 4.73TIAEE510 pKa = 4.9FIPAIPLAHH519 pKa = 7.25PAPSLAFSDD528 pKa = 4.13RR529 pKa = 11.84VEE531 pKa = 4.53SYY533 pKa = 10.33PVSPVNDD540 pKa = 3.39EE541 pKa = 4.12VFNMIVLTKK550 pKa = 10.74

Molecular weight: 59.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099J168|A0A099J168_9MICO DUF4190 domain-containing protein OS=Cryobacterium roopkundense OX=1001240 GN=GY21_18155 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.33VRR4 pKa = 11.84NSLKK8 pKa = 10.39ALKK11 pKa = 9.59KK12 pKa = 10.37VPGSQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 8.63LNPRR34 pKa = 11.84AKK36 pKa = 10.25ARR38 pKa = 11.84QGG40 pKa = 3.19

Molecular weight: 4.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3249

0

3249

988510

37

1997

304.3

32.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.836 ± 0.054

0.583 ± 0.011

5.788 ± 0.031

5.303 ± 0.039

3.314 ± 0.025

8.744 ± 0.039

2.015 ± 0.021

4.864 ± 0.03

2.239 ± 0.032

10.447 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.929 ± 0.017

2.421 ± 0.023

5.106 ± 0.03

2.917 ± 0.022

6.649 ± 0.04

6.117 ± 0.031

6.402 ± 0.034

8.849 ± 0.042

1.423 ± 0.017

2.053 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski