Wuhan fly virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLL7|A0A1L3KLL7_9VIRU Uncharacterized protein OS=Wuhan fly virus 5 OX=1923699 PE=4 SV=1
MM1 pKa = 7.61SRR3 pKa = 11.84YY4 pKa = 9.82NFNNNNNGGNFQGFVPDD21 pKa = 4.2FNNWRR26 pKa = 11.84DD27 pKa = 3.44PNMSYY32 pKa = 10.53QYY34 pKa = 11.38NNQQQQRR41 pKa = 11.84PQQNFGPQRR50 pKa = 11.84GGRR53 pKa = 11.84PHH55 pKa = 6.82VFRR58 pKa = 11.84GRR60 pKa = 11.84GGRR63 pKa = 11.84RR64 pKa = 11.84GGISNNPKK72 pKa = 10.06ANVRR76 pKa = 11.84NPVEE80 pKa = 4.48LKK82 pKa = 10.85EE83 pKa = 4.12EE84 pKa = 4.38TPKK87 pKa = 9.47PTTMPTGQHH96 pKa = 5.55LSKK99 pKa = 10.1TNSEE103 pKa = 4.54FKK105 pKa = 10.72NDD107 pKa = 4.01PLYY110 pKa = 11.31LEE112 pKa = 5.52AFMPSPSEE120 pKa = 3.71EE121 pKa = 3.94LVPIAEE127 pKa = 4.58EE128 pKa = 4.31LNHH131 pKa = 6.33FPGFDD136 pKa = 3.31GLTTLIEE143 pKa = 3.88EE144 pKa = 4.58TYY146 pKa = 11.12EE147 pKa = 3.92NFSAHH152 pKa = 5.88NLNFKK157 pKa = 10.62RR158 pKa = 11.84SVSLSAYY165 pKa = 9.57SYY167 pKa = 10.86YY168 pKa = 9.53ITVLSWARR176 pKa = 11.84VLYY179 pKa = 9.6LKK181 pKa = 10.84KK182 pKa = 9.48MNKK185 pKa = 9.51YY186 pKa = 10.45KK187 pKa = 10.14LTTNEE192 pKa = 3.98LEE194 pKa = 4.64FIEE197 pKa = 4.27MVYY200 pKa = 10.51EE201 pKa = 3.86QGNFLLPKK209 pKa = 10.21SVIIYY214 pKa = 10.45LSGFGNFNIPSGVEE228 pKa = 3.8SKK230 pKa = 11.15FNLKK234 pKa = 10.25PYY236 pKa = 10.01TYY238 pKa = 10.61DD239 pKa = 3.18EE240 pKa = 4.58LGYY243 pKa = 10.62FVDD246 pKa = 5.92FDD248 pKa = 4.16TKK250 pKa = 10.77FYY252 pKa = 11.15LCTSYY257 pKa = 11.38PNISIAAEE265 pKa = 3.5RR266 pKa = 11.84VMRR269 pKa = 11.84DD270 pKa = 3.19LAYY273 pKa = 9.83TDD275 pKa = 3.46NPEE278 pKa = 4.41IGEE281 pKa = 4.12AWSPAEE287 pKa = 6.16IEE289 pKa = 4.38QEE291 pKa = 3.84WNTRR295 pKa = 11.84CIGYY299 pKa = 9.8APSVIIHH306 pKa = 6.51DD307 pKa = 3.84MQRR310 pKa = 11.84AVLEE314 pKa = 4.22RR315 pKa = 11.84ALVRR319 pKa = 11.84SDD321 pKa = 4.55NFPSDD326 pKa = 3.79LNGFLVNVRR335 pKa = 11.84LMNNIQKK342 pKa = 9.67YY343 pKa = 9.67LSEE346 pKa = 4.32IPSLEE351 pKa = 4.09SGPIPKK357 pKa = 10.07NLTGSLGQFIIEE369 pKa = 4.24IPTVSVGANDD379 pKa = 4.28EE380 pKa = 4.2IGSLSFTSKK389 pKa = 10.96SPLSCPGSMSFLGGSFLYY407 pKa = 10.38RR408 pKa = 11.84VDD410 pKa = 4.66KK411 pKa = 11.03DD412 pKa = 3.31LTPARR417 pKa = 11.84RR418 pKa = 11.84KK419 pKa = 10.05YY420 pKa = 10.22FYY422 pKa = 9.93PYY424 pKa = 10.13TKK426 pKa = 10.68DD427 pKa = 3.53SDD429 pKa = 3.94TQEE432 pKa = 4.69DD433 pKa = 4.7KK434 pKa = 11.39INLNLLNTGWSPIFNQIYY452 pKa = 9.59HH453 pKa = 5.92YY454 pKa = 11.24SNVPFKK460 pKa = 10.73PVLRR464 pKa = 11.84VKK466 pKa = 10.05KK467 pKa = 10.18FCSIDD472 pKa = 3.54VKK474 pKa = 10.99PSGVV478 pKa = 3.13

Molecular weight: 54.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLL7|A0A1L3KLL7_9VIRU Uncharacterized protein OS=Wuhan fly virus 5 OX=1923699 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84SRR4 pKa = 11.84GPATRR9 pKa = 11.84QCINVSPRR17 pKa = 11.84RR18 pKa = 11.84GTKK21 pKa = 8.16TAITRR26 pKa = 11.84LEE28 pKa = 4.1NKK30 pKa = 9.74EE31 pKa = 4.03GFVMNMTPARR41 pKa = 11.84EE42 pKa = 4.02DD43 pKa = 3.03GKK45 pKa = 11.15ARR47 pKa = 11.84LACEE51 pKa = 4.58LYY53 pKa = 10.28FGKK56 pKa = 10.4DD57 pKa = 2.72ITDD60 pKa = 4.72KK61 pKa = 10.35ITSTLHH67 pKa = 6.69RR68 pKa = 11.84SRR70 pKa = 11.84LTYY73 pKa = 10.7DD74 pKa = 3.24SLLEE78 pKa = 4.65DD79 pKa = 3.82FMDD82 pKa = 3.8YY83 pKa = 11.32DD84 pKa = 3.79RR85 pKa = 11.84THH87 pKa = 6.6VPRR90 pKa = 11.84ISDD93 pKa = 3.52PDD95 pKa = 3.25AQIIYY100 pKa = 10.06KK101 pKa = 10.42CVLQSIKK108 pKa = 10.87DD109 pKa = 3.51DD110 pKa = 4.01LGNVKK115 pKa = 10.48LSPLTYY121 pKa = 9.85QQVRR125 pKa = 11.84DD126 pKa = 3.89LPDD129 pKa = 3.64FPGAKK134 pKa = 9.94SPGLPYY140 pKa = 10.74KK141 pKa = 10.81NLGFTKK147 pKa = 10.45KK148 pKa = 10.41SDD150 pKa = 3.58VYY152 pKa = 11.11DD153 pKa = 3.89DD154 pKa = 4.7PIYY157 pKa = 10.5RR158 pKa = 11.84EE159 pKa = 4.05HH160 pKa = 6.94LTNLWRR166 pKa = 11.84NIGHH170 pKa = 6.73GKK172 pKa = 8.77NVKK175 pKa = 10.45LPDD178 pKa = 3.05VCMFARR184 pKa = 11.84AQVAKK189 pKa = 10.45APKK192 pKa = 9.59NKK194 pKa = 8.93IRR196 pKa = 11.84ATWGYY201 pKa = 10.4SFDD204 pKa = 3.69VYY206 pKa = 10.53MEE208 pKa = 4.19EE209 pKa = 4.97ARR211 pKa = 11.84FFYY214 pKa = 10.03PIQEE218 pKa = 4.4FIKK221 pKa = 9.9SHH223 pKa = 4.97KK224 pKa = 9.78HH225 pKa = 4.88NLPIAYY231 pKa = 9.24GLEE234 pKa = 4.01MANGGMVAINDD245 pKa = 3.29MLQRR249 pKa = 11.84HH250 pKa = 5.94RR251 pKa = 11.84NSKK254 pKa = 10.28YY255 pKa = 10.93VISDD259 pKa = 2.88WSKK262 pKa = 9.92FDD264 pKa = 3.25KK265 pKa = 10.72TIPPWLIRR273 pKa = 11.84DD274 pKa = 3.46AFEE277 pKa = 4.35ILEE280 pKa = 3.97GLIDD284 pKa = 4.31FSAVHH289 pKa = 6.53RR290 pKa = 11.84SAHH293 pKa = 4.99EE294 pKa = 3.52KK295 pKa = 10.49RR296 pKa = 11.84RR297 pKa = 11.84FKK299 pKa = 11.06KK300 pKa = 10.26LVDD303 pKa = 3.85YY304 pKa = 10.48FVEE307 pKa = 4.67TPIRR311 pKa = 11.84TCKK314 pKa = 10.63GEE316 pKa = 3.98RR317 pKa = 11.84FLVTGGVPSGSCFTNIIDD335 pKa = 5.23SIINCIVTRR344 pKa = 11.84FLVFQTTNAFPVGEE358 pKa = 4.59IFLGDD363 pKa = 3.67DD364 pKa = 3.54GVFIVDD370 pKa = 4.62GFACLEE376 pKa = 4.98DD377 pKa = 3.63IASLAIKK384 pKa = 9.83YY385 pKa = 10.19FGMILNVDD393 pKa = 3.4KK394 pKa = 11.19SYY396 pKa = 10.2VTTNPSNVHH405 pKa = 4.86FLGYY409 pKa = 10.08FNYY412 pKa = 10.41SGYY415 pKa = 9.82PFKK418 pKa = 11.38NQDD421 pKa = 3.13FLIASFIFPEE431 pKa = 4.29HH432 pKa = 6.29KK433 pKa = 9.4RR434 pKa = 11.84TRR436 pKa = 11.84LVDD439 pKa = 3.48ACAAALGQMYY449 pKa = 10.5SGFDD453 pKa = 3.05PGYY456 pKa = 10.41ARR458 pKa = 11.84NWLNIIHH465 pKa = 6.25YY466 pKa = 10.09LADD469 pKa = 4.23LEE471 pKa = 4.27NSNPFSLNEE480 pKa = 4.05VVLHH484 pKa = 6.16LKK486 pKa = 10.57NNEE489 pKa = 3.6FRR491 pKa = 11.84HH492 pKa = 6.11KK493 pKa = 10.65YY494 pKa = 8.47LAQVGISADD503 pKa = 3.56SMTLPSRR510 pKa = 11.84ANSQILEE517 pKa = 4.34VLPKK521 pKa = 9.04TYY523 pKa = 10.88CNITLPSRR531 pKa = 11.84AYY533 pKa = 10.18DD534 pKa = 3.59FKK536 pKa = 11.45DD537 pKa = 2.76ILSRR541 pKa = 11.84II542 pKa = 4.05

Molecular weight: 61.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1020

478

542

510.0

58.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.902 ± 1.026

1.373 ± 0.353

5.49 ± 1.0

5.098 ± 0.777

6.275 ± 0.001

6.176 ± 0.342

1.961 ± 0.465

6.471 ± 0.68

5.784 ± 0.366

8.431 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.043

7.255 ± 1.286

6.176 ± 0.756

2.941 ± 0.406

5.588 ± 0.512

7.353 ± 0.808

5.0 ± 0.124

5.588 ± 0.098

0.98 ± 0.043

5.0 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski