Narcissus mosaic virus (NMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P15097|TGB2_NMV Movement protein TGB2 OS=Narcissus mosaic virus OX=12180 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.7DD2 pKa = 5.22CKK4 pKa = 11.04YY5 pKa = 10.82LLEE8 pKa = 4.91LLDD11 pKa = 4.05SYY13 pKa = 11.78SFIRR17 pKa = 11.84SSRR20 pKa = 11.84SFSSPIIIHH29 pKa = 6.32GVAGCGKK36 pKa = 8.64STIIQKK42 pKa = 9.94IALAFPEE49 pKa = 4.28LLIGSFTPALLDD61 pKa = 3.79SNSGRR66 pKa = 11.84KK67 pKa = 8.17QLAVTSDD74 pKa = 3.6PLDD77 pKa = 3.53ILDD80 pKa = 4.86EE81 pKa = 4.26YY82 pKa = 11.36LGGPNPVVRR91 pKa = 11.84LAKK94 pKa = 10.49FCDD97 pKa = 3.58PLQYY101 pKa = 10.59SCEE104 pKa = 4.13QPEE107 pKa = 4.66VPHH110 pKa = 5.6FTSLLTWRR118 pKa = 11.84FCVRR122 pKa = 11.84TTALLNGIFGCQIKK136 pKa = 10.23SRR138 pKa = 11.84RR139 pKa = 11.84EE140 pKa = 3.91DD141 pKa = 3.51LCHH144 pKa = 6.42LTHH147 pKa = 6.94EE148 pKa = 4.75NPYY151 pKa = 8.04TTDD154 pKa = 3.22PKK156 pKa = 10.93GVVVAHH162 pKa = 5.48EE163 pKa = 4.08QEE165 pKa = 4.75VINLLLQHH173 pKa = 6.81GCPVTPTQHH182 pKa = 6.34LWGLTIPVVSVYY194 pKa = 8.77ITSIASLSTVDD205 pKa = 3.81RR206 pKa = 11.84ANLFLSLTRR215 pKa = 11.84DD216 pKa = 3.26SKK218 pKa = 11.3ALHH221 pKa = 6.27IFEE224 pKa = 4.56FDD226 pKa = 3.1AWSHH230 pKa = 5.17ATCC233 pKa = 5.11

Molecular weight: 25.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15098|TGB3_NMV Movement protein TGBp3 OS=Narcissus mosaic virus OX=12180 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.83PGLTPPVNYY10 pKa = 9.63EE11 pKa = 3.52QVYY14 pKa = 9.64KK15 pKa = 10.95VLAIGFLLCASIYY28 pKa = 9.71CLRR31 pKa = 11.84SNHH34 pKa = 6.1LPHH37 pKa = 7.98VGDD40 pKa = 4.89NIHH43 pKa = 6.38SLPHH47 pKa = 5.95GGNYY51 pKa = 10.23ADD53 pKa = 3.56GTKK56 pKa = 9.71RR57 pKa = 11.84VQYY60 pKa = 9.13FRR62 pKa = 11.84PHH64 pKa = 6.49SSTSTNHH71 pKa = 7.2KK72 pKa = 8.79YY73 pKa = 8.12TALCAVLTLSLLIFAQTRR91 pKa = 11.84LAAGNRR97 pKa = 11.84ITSVSICHH105 pKa = 6.31HH106 pKa = 6.74CSSQGSLSGGNHH118 pKa = 5.16GRR120 pKa = 11.84VSGHH124 pKa = 5.97SEE126 pKa = 4.06LPTTT130 pKa = 4.58

Molecular weight: 14.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2440

94

1643

406.7

45.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.582 ± 1.086

2.295 ± 0.712

5.082 ± 0.993

4.959 ± 1.291

4.344 ± 0.532

4.057 ± 0.857

2.992 ± 0.69

5.369 ± 0.916

5.82 ± 1.274

10.861 ± 1.117

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.528

3.852 ± 0.329

6.639 ± 0.762

4.139 ± 0.416

4.795 ± 0.155

8.033 ± 1.206

7.213 ± 0.411

5.615 ± 0.462

1.27 ± 0.186

3.033 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski