Paenibacillus phage Dragolir

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gochnauervirinae; Dragolirvirus; Paenibacillus virus Dragolir

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7SC57|A0A2I7SC57_9CAUD Uncharacterized protein OS=Paenibacillus phage Dragolir OX=2070190 GN=DRAGOLIR_32 PE=4 SV=1
MM1 pKa = 7.48NEE3 pKa = 3.99TVEE6 pKa = 4.49CPYY9 pKa = 10.76CEE11 pKa = 4.77HH12 pKa = 6.81EE13 pKa = 4.45NDD15 pKa = 3.39MSHH18 pKa = 7.13ALVDD22 pKa = 4.1GLSDD26 pKa = 5.0DD27 pKa = 4.07NTFDD31 pKa = 3.63WEE33 pKa = 4.58CNNCHH38 pKa = 6.31EE39 pKa = 4.29EE40 pKa = 4.26FEE42 pKa = 4.81VKK44 pKa = 10.67VEE46 pKa = 4.14FEE48 pKa = 4.43PSFSASKK55 pKa = 10.33IEE57 pKa = 4.52YY58 pKa = 9.35IDD60 pKa = 5.34CEE62 pKa = 4.09HH63 pKa = 7.09CGNNTRR69 pKa = 11.84DD70 pKa = 3.25IYY72 pKa = 11.12EE73 pKa = 4.31KK74 pKa = 10.6GRR76 pKa = 11.84VYY78 pKa = 10.33PFPEE82 pKa = 4.27RR83 pKa = 11.84LSGKK87 pKa = 9.11RR88 pKa = 11.84VCKK91 pKa = 10.34QCFCEE96 pKa = 4.87SLAEE100 pKa = 4.28EE101 pKa = 4.27YY102 pKa = 10.29TSNKK106 pKa = 9.42KK107 pKa = 9.92VDD109 pKa = 3.11

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7SC65|A0A2I7SC65_9CAUD Uncharacterized protein OS=Paenibacillus phage Dragolir OX=2070190 GN=DRAGOLIR_13 PE=4 SV=1
MM1 pKa = 7.15SWIAMRR7 pKa = 11.84EE8 pKa = 3.55VDD10 pKa = 3.7PYY12 pKa = 11.03SRR14 pKa = 11.84RR15 pKa = 11.84GNGRR19 pKa = 11.84WADD22 pKa = 3.67EE23 pKa = 3.82VRR25 pKa = 11.84ILILFSSYY33 pKa = 10.74VIQGKK38 pKa = 9.02RR39 pKa = 2.96

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12741

23

1233

187.4

21.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.734 ± 0.59

1.012 ± 0.165

5.525 ± 0.294

8.225 ± 0.417

3.681 ± 0.272

6.609 ± 0.238

1.774 ± 0.175

6.334 ± 0.371

7.872 ± 0.326

8.814 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.154

4.552 ± 0.236

3.485 ± 0.205

4.505 ± 0.23

5.125 ± 0.379

5.73 ± 0.275

5.455 ± 0.277

6.868 ± 0.264

1.593 ± 0.168

3.406 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski