Mycoplasma cloacale

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z4LMB3|A0A2Z4LMB3_9MOLU Uncharacterized protein OS=Mycoplasma cloacale OX=92401 GN=DK849_02485 PE=4 SV=1
MM1 pKa = 7.34TNKK4 pKa = 10.19KK5 pKa = 10.0YY6 pKa = 10.62FPLTKK11 pKa = 10.37EE12 pKa = 3.95EE13 pKa = 4.25LKK15 pKa = 11.08EE16 pKa = 3.82LVNNLDD22 pKa = 3.84IYY24 pKa = 11.17LGDD27 pKa = 3.69IDD29 pKa = 4.12TSRR32 pKa = 11.84MFDD35 pKa = 3.23MSSLFQNSLRR45 pKa = 11.84KK46 pKa = 9.98KK47 pKa = 10.15FDD49 pKa = 4.72GIEE52 pKa = 3.82TWNTSNVLNMEE63 pKa = 4.2RR64 pKa = 11.84MFEE67 pKa = 3.99SSFINKK73 pKa = 8.73PLNFDD78 pKa = 3.53TSNVKK83 pKa = 10.3NMEE86 pKa = 4.01YY87 pKa = 10.03MFADD91 pKa = 4.62CYY93 pKa = 11.22LFNQPINFNTSKK105 pKa = 10.34VKK107 pKa = 10.56NMKK110 pKa = 10.5NMFSNCYY117 pKa = 9.87FFNQNLNWDD126 pKa = 3.83TSNVKK131 pKa = 10.19NMSYY135 pKa = 9.54MFEE138 pKa = 4.1NCHH141 pKa = 6.75SFNQPLNWDD150 pKa = 3.67TSNVEE155 pKa = 3.63NMSYY159 pKa = 9.64MFRR162 pKa = 11.84GCHH165 pKa = 6.06SFNQPLDD172 pKa = 3.39WDD174 pKa = 4.25TTNLTDD180 pKa = 3.69TYY182 pKa = 11.5SMFSGCRR189 pKa = 11.84SFNQPLNWDD198 pKa = 3.79TTNVEE203 pKa = 3.98DD204 pKa = 3.57MSYY207 pKa = 9.58MFEE210 pKa = 4.21YY211 pKa = 10.36CHH213 pKa = 7.11SFNQPLNWDD222 pKa = 3.67TSNVEE227 pKa = 3.88NMSLMFYY234 pKa = 9.93YY235 pKa = 10.28CHH237 pKa = 7.29SFNQPLNWDD246 pKa = 3.72TSNVEE251 pKa = 4.44DD252 pKa = 3.71MSKK255 pKa = 9.4MFEE258 pKa = 3.94YY259 pKa = 10.26CYY261 pKa = 10.96SFNQPLDD268 pKa = 3.35WDD270 pKa = 3.99TSNVEE275 pKa = 4.28DD276 pKa = 4.1MSDD279 pKa = 3.06MFEE282 pKa = 4.19NCHH285 pKa = 6.73SFNQPLNWDD294 pKa = 3.67TSNVEE299 pKa = 3.77NMAGMFKK306 pKa = 10.37NCHH309 pKa = 6.03SFNQPLDD316 pKa = 3.46WDD318 pKa = 3.93TSNVKK323 pKa = 10.42DD324 pKa = 3.81MNFMFCEE331 pKa = 4.56CSAFNQPLDD340 pKa = 3.39WDD342 pKa = 4.08TTNVEE347 pKa = 3.8NMEE350 pKa = 4.1YY351 pKa = 10.05MFWNCKK357 pKa = 6.9EE358 pKa = 4.56LEE360 pKa = 4.06YY361 pKa = 11.18EE362 pKa = 4.55MNFKK366 pKa = 9.95MPYY369 pKa = 10.12NKK371 pKa = 9.03TKK373 pKa = 11.09NMFHH377 pKa = 6.71GCILLSNEE385 pKa = 4.16EE386 pKa = 4.1EE387 pKa = 4.29DD388 pKa = 5.34NEE390 pKa = 4.56TKK392 pKa = 10.42HH393 pKa = 6.42SKK395 pKa = 9.2EE396 pKa = 3.61WFNDD400 pKa = 3.02QKK402 pKa = 11.17EE403 pKa = 4.1IDD405 pKa = 4.88EE406 pKa = 4.48IPLLKK411 pKa = 10.47FKK413 pKa = 10.58IIEE416 pKa = 4.33NDD418 pKa = 3.83DD419 pKa = 3.71DD420 pKa = 5.12DD421 pKa = 4.88EE422 pKa = 4.35NN423 pKa = 4.41

Molecular weight:
50.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z4LLZ1|A0A2Z4LLZ1_9MOLU 30S ribosomal protein S5 OS=Mycoplasma cloacale OX=92401 GN=rpsE PE=3 SV=1
MM1 pKa = 7.33ARR3 pKa = 11.84KK4 pKa = 9.63ALMVKK9 pKa = 10.38AEE11 pKa = 4.31RR12 pKa = 11.84EE13 pKa = 4.05PKK15 pKa = 10.35FGVRR19 pKa = 11.84KK20 pKa = 7.54YY21 pKa = 8.6TRR23 pKa = 11.84CQICGRR29 pKa = 11.84VHH31 pKa = 6.76AVLRR35 pKa = 11.84KK36 pKa = 9.58YY37 pKa = 10.23KK38 pKa = 9.85ICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.88LAHH49 pKa = 6.54EE50 pKa = 4.41GKK52 pKa = 10.21IPGVKK57 pKa = 9.38KK58 pKa = 10.89ASWW61 pKa = 3.03

Molecular weight:
7.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

539

0

539

201187

37

2772

373.3

42.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.257 ± 0.116

0.596 ± 0.03

5.439 ± 0.082

7.068 ± 0.106

5.055 ± 0.107

4.324 ± 0.105

1.614 ± 0.037

10.169 ± 0.124

9.773 ± 0.092

9.598 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.882 ± 0.051

8.237 ± 0.166

2.656 ± 0.058

3.232 ± 0.049

2.889 ± 0.059

6.26 ± 0.075

5.301 ± 0.072

5.244 ± 0.071

0.941 ± 0.036

4.463 ± 0.072

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski