Tetrapisispora blattae (strain ATCC 34711 / CBS 6284 / DSM 70876 / NBRC 10599 / NRRL Y-10934 / UCD 77-7) (Yeast) (Kluyveromyces blattae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Tetrapisispora; Tetrapisispora blattae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5385 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2H1V5|I2H1V5_TETBL Uncharacterized protein OS=Tetrapisispora blattae (strain ATCC 34711 / CBS 6284 / DSM 70876 / NBRC 10599 / NRRL Y-10934 / UCD 77-7) OX=1071380 GN=TBLA0C05590 PE=4 SV=1
MM1 pKa = 7.49NLTPNIQLTQDD12 pKa = 3.64CAPAKK17 pKa = 10.65DD18 pKa = 3.87DD19 pKa = 3.85DD20 pKa = 4.48EE21 pKa = 6.07EE22 pKa = 4.64YY23 pKa = 10.77FSCISNLDD31 pKa = 3.29ATGQSSFQSKK41 pKa = 8.51QVLNNQLNPSKK52 pKa = 10.77YY53 pKa = 8.49YY54 pKa = 10.66NNLSFHH60 pKa = 7.0HH61 pKa = 6.18STVSSSSNSATTQFHH76 pKa = 6.7NISFKK81 pKa = 10.79KK82 pKa = 9.99KK83 pKa = 7.2QLKK86 pKa = 10.03FKK88 pKa = 10.5VFQKK92 pKa = 10.32CYY94 pKa = 9.61PRR96 pKa = 11.84TPVPQNEE103 pKa = 4.88GCCQDD108 pKa = 3.6YY109 pKa = 9.32TSLCLEE115 pKa = 4.44DD116 pKa = 4.79SNNEE120 pKa = 4.01EE121 pKa = 4.01YY122 pKa = 10.95DD123 pKa = 3.62DD124 pKa = 6.58DD125 pKa = 4.56EE126 pKa = 7.48DD127 pKa = 4.27EE128 pKa = 6.66DD129 pKa = 4.17EE130 pKa = 6.63DD131 pKa = 4.21EE132 pKa = 6.65DD133 pKa = 4.31EE134 pKa = 6.65DD135 pKa = 4.31EE136 pKa = 6.65DD137 pKa = 4.31EE138 pKa = 6.65DD139 pKa = 4.31EE140 pKa = 6.65DD141 pKa = 4.31EE142 pKa = 6.65DD143 pKa = 4.31EE144 pKa = 6.65DD145 pKa = 4.31EE146 pKa = 6.6DD147 pKa = 4.38EE148 pKa = 6.51DD149 pKa = 4.15EE150 pKa = 6.21DD151 pKa = 4.08EE152 pKa = 5.3YY153 pKa = 11.34EE154 pKa = 5.08YY155 pKa = 11.22EE156 pKa = 6.08DD157 pKa = 3.94EE158 pKa = 6.58DD159 pKa = 4.38EE160 pKa = 6.48DD161 pKa = 5.16EE162 pKa = 6.12DD163 pKa = 5.4EE164 pKa = 5.09DD165 pKa = 5.82DD166 pKa = 4.1EE167 pKa = 5.65GKK169 pKa = 11.09GSTGTYY175 pKa = 9.26YY176 pKa = 10.74RR177 pKa = 11.84KK178 pKa = 9.71QKK180 pKa = 10.06YY181 pKa = 8.85

Molecular weight: 21.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2GY00|I2GY00_TETBL ATP-dependent RNA helicase DBP10 OS=Tetrapisispora blattae (strain ATCC 34711 / CBS 6284 / DSM 70876 / NBRC 10599 / NRRL Y-10934 / UCD 77-7) OX=1071380 GN=TBLA0B01590 PE=3 SV=1
MM1 pKa = 7.41LRR3 pKa = 11.84LIGLFAAPSHH13 pKa = 6.37LPAPLQLSLVRR24 pKa = 11.84GLHH27 pKa = 5.04KK28 pKa = 10.83LKK30 pKa = 10.93LKK32 pKa = 6.52THH34 pKa = 6.66KK35 pKa = 9.91GTLKK39 pKa = 9.0RR40 pKa = 11.84WRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84VAPGGPAAEE53 pKa = 3.71QFVRR57 pKa = 11.84SKK59 pKa = 11.03AGRR62 pKa = 11.84NHH64 pKa = 6.8GNIGWSHH71 pKa = 6.66RR72 pKa = 11.84ALAALSRR79 pKa = 11.84RR80 pKa = 11.84VPAHH84 pKa = 4.89STHH87 pKa = 5.66VKK89 pKa = 10.24ALRR92 pKa = 11.84KK93 pKa = 9.48LLPYY97 pKa = 10.64

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5385

0

5385

2914559

25

4917

541.2

61.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.67 ± 0.034

1.11 ± 0.011

5.905 ± 0.026

6.121 ± 0.035

4.321 ± 0.022

4.377 ± 0.029

2.059 ± 0.013

7.524 ± 0.035

7.435 ± 0.037

9.365 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.978 ± 0.011

8.044 ± 0.059

4.251 ± 0.026

3.96 ± 0.03

3.866 ± 0.02

9.456 ± 0.053

6.352 ± 0.038

4.836 ± 0.026

0.937 ± 0.009

3.43 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski