Halanaerobium praevalens (strain ATCC 33744 / DSM 2228 / GSL)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium; Halanaerobium praevalens

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3DQN5|E3DQN5_HALPG Outer membrane efflux protein OS=Halanaerobium praevalens (strain ATCC 33744 / DSM 2228 / GSL) OX=572479 GN=Hprae_1821 PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.54SKK4 pKa = 10.61ILVLFLAAFLVIGLSSASMAQVPGYY29 pKa = 9.77WDD31 pKa = 3.06IKK33 pKa = 10.0GAIDD37 pKa = 3.84FGGEE41 pKa = 3.42IDD43 pKa = 4.39IDD45 pKa = 3.96GASDD49 pKa = 3.51EE50 pKa = 5.29DD51 pKa = 4.04VDD53 pKa = 5.32SGFTLVGEE61 pKa = 4.33YY62 pKa = 10.01KK63 pKa = 10.63VPYY66 pKa = 8.21TSQWTLGAGMRR77 pKa = 11.84YY78 pKa = 9.27QLDD81 pKa = 3.73RR82 pKa = 11.84EE83 pKa = 4.18TDD85 pKa = 3.46DD86 pKa = 3.54TKK88 pKa = 11.56KK89 pKa = 10.79DD90 pKa = 3.55FNFIPFYY97 pKa = 11.37GLAQYY102 pKa = 11.4NMQNSPYY109 pKa = 9.95YY110 pKa = 10.75LLGHH114 pKa = 7.17LGYY117 pKa = 8.55NTFDD121 pKa = 3.21IDD123 pKa = 3.93TYY125 pKa = 10.79SDD127 pKa = 3.54EE128 pKa = 4.63SGGMYY133 pKa = 10.01YY134 pKa = 10.73AVGAGMDD141 pKa = 3.83LASNMSAEE149 pKa = 4.14VMYY152 pKa = 10.86SVNNGEE158 pKa = 4.57AEE160 pKa = 4.55VKK162 pKa = 9.76GTDD165 pKa = 3.44QDD167 pKa = 3.84VEE169 pKa = 4.32YY170 pKa = 11.29SKK172 pKa = 10.72LTVSLGYY179 pKa = 10.31QFF181 pKa = 5.42

Molecular weight: 19.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3DLB5|E3DLB5_HALPG Ribosomal protein S12 methylthiotransferase RimO OS=Halanaerobium praevalens (strain ATCC 33744 / DSM 2228 / GSL) OX=572479 GN=rimO PE=3 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84GRR5 pKa = 11.84NKK7 pKa = 10.31SCHH10 pKa = 5.01FCANEE15 pKa = 3.94NKK17 pKa = 10.51KK18 pKa = 9.71IDD20 pKa = 3.95YY21 pKa = 10.38KK22 pKa = 10.61DD23 pKa = 3.61LRR25 pKa = 11.84TLNRR29 pKa = 11.84YY30 pKa = 7.79LTDD33 pKa = 3.45RR34 pKa = 11.84GKK36 pKa = 10.5IMPRR40 pKa = 11.84RR41 pKa = 11.84VTGNCSKK48 pKa = 10.36HH49 pKa = 5.42QKK51 pKa = 10.45LITKK55 pKa = 9.09AVKK58 pKa = 9.94RR59 pKa = 11.84ARR61 pKa = 11.84SVALLPYY68 pKa = 10.22VKK70 pKa = 10.27EE71 pKa = 4.05

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2055

0

2055

674614

30

2607

328.3

36.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.581 ± 0.054

0.606 ± 0.017

5.238 ± 0.044

7.776 ± 0.061

4.455 ± 0.042

6.143 ± 0.057

1.388 ± 0.018

8.951 ± 0.049

9.126 ± 0.071

10.332 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.027

5.679 ± 0.055

2.998 ± 0.03

3.575 ± 0.038

3.42 ± 0.035

5.883 ± 0.04

4.432 ± 0.034

5.845 ± 0.049

0.68 ± 0.017

3.659 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski