Lake Sarah-associated circular virus-38

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQP9|A0A140AQP9_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-38 OX=1685766 PE=3 SV=1
MM1 pKa = 7.09SNKK4 pKa = 8.41MAKK7 pKa = 8.4NWCMTLNNYY16 pKa = 9.89DD17 pKa = 3.76DD18 pKa = 5.03SEE20 pKa = 4.36VARR23 pKa = 11.84FEE25 pKa = 4.05SHH27 pKa = 4.75MRR29 pKa = 11.84PWCVYY34 pKa = 8.89YY35 pKa = 9.96IYY37 pKa = 10.84GFEE40 pKa = 4.12RR41 pKa = 11.84GEE43 pKa = 3.92NDD45 pKa = 3.73TPHH48 pKa = 6.33LQCFFSLKK56 pKa = 9.77AKK58 pKa = 10.43KK59 pKa = 10.17RR60 pKa = 11.84MSCLKK65 pKa = 10.39KK66 pKa = 9.94IFPRR70 pKa = 11.84AHH72 pKa = 6.2FEE74 pKa = 4.24VKK76 pKa = 10.16SRR78 pKa = 11.84ASTMEE83 pKa = 3.77QASDD87 pKa = 3.67YY88 pKa = 10.66CKK90 pKa = 10.57KK91 pKa = 10.56EE92 pKa = 3.84EE93 pKa = 4.8NFIEE97 pKa = 4.39WGVLPDD103 pKa = 3.41NSTARR108 pKa = 11.84GLKK111 pKa = 10.2AISDD115 pKa = 4.0NYY117 pKa = 10.69EE118 pKa = 3.86EE119 pKa = 4.33TVEE122 pKa = 4.0LAKK125 pKa = 10.95KK126 pKa = 10.71GDD128 pKa = 3.47IEE130 pKa = 4.98AINPEE135 pKa = 4.48HH136 pKa = 6.14VLKK139 pKa = 10.78YY140 pKa = 10.1YY141 pKa = 8.54PTIKK145 pKa = 10.37RR146 pKa = 11.84IAHH149 pKa = 6.65DD150 pKa = 4.0NKK152 pKa = 10.79KK153 pKa = 9.54MPSDD157 pKa = 3.86LLWEE161 pKa = 4.67EE162 pKa = 4.13GHH164 pKa = 7.07PPNIWIYY171 pKa = 10.79GPTGTGKK178 pKa = 10.22SYY180 pKa = 10.43RR181 pKa = 11.84ARR183 pKa = 11.84AILQEE188 pKa = 4.08NFGQFYY194 pKa = 11.26SKK196 pKa = 10.13MAQNKK201 pKa = 7.55WWDD204 pKa = 3.62KK205 pKa = 10.01YY206 pKa = 11.18DD207 pKa = 3.7GEE209 pKa = 4.37EE210 pKa = 4.24GVLIEE215 pKa = 6.28DD216 pKa = 3.65MDD218 pKa = 3.9ILHH221 pKa = 6.86NYY223 pKa = 6.96MGPYY227 pKa = 8.25MKK229 pKa = 9.78IWADD233 pKa = 3.14KK234 pKa = 9.03YY235 pKa = 10.74AFPVEE240 pKa = 4.52VKK242 pKa = 10.07TSGDD246 pKa = 3.55RR247 pKa = 11.84IRR249 pKa = 11.84PKK251 pKa = 10.78VIVVTSNYY259 pKa = 9.38TIEE262 pKa = 4.58QIWPDD267 pKa = 3.71RR268 pKa = 11.84STHH271 pKa = 5.32GPISRR276 pKa = 11.84RR277 pKa = 11.84FKK279 pKa = 10.35VIHH282 pKa = 6.68MDD284 pKa = 3.6QPWNANINQVLRR296 pKa = 11.84DD297 pKa = 3.95APAEE301 pKa = 3.95TSAPRR306 pKa = 11.84EE307 pKa = 4.2KK308 pKa = 10.21KK309 pKa = 10.38RR310 pKa = 11.84KK311 pKa = 9.08FDD313 pKa = 3.68QPLKK317 pKa = 10.75KK318 pKa = 9.76PALLRR323 pKa = 11.84RR324 pKa = 11.84NAVGDD329 pKa = 3.56LVEE332 pKa = 4.11THH334 pKa = 7.28GYY336 pKa = 9.59QPQQTIPQYY345 pKa = 11.02LEE347 pKa = 3.72PTLEE351 pKa = 3.99EE352 pKa = 3.95LAEE355 pKa = 3.94EE356 pKa = 4.22MEE358 pKa = 4.87IYY360 pKa = 10.2RR361 pKa = 11.84ASQDD365 pKa = 3.22MFEE368 pKa = 4.77EE369 pKa = 4.57LSEE372 pKa = 4.88SDD374 pKa = 5.51DD375 pKa = 5.05LLDD378 pKa = 4.29LL379 pKa = 4.89

Molecular weight: 44.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQP9|A0A140AQP9_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-38 OX=1685766 PE=3 SV=1
MM1 pKa = 8.12DD2 pKa = 5.06GNGDD6 pKa = 3.83VPEE9 pKa = 4.67GWTDD13 pKa = 5.12PITMAEE19 pKa = 3.4IRR21 pKa = 11.84KK22 pKa = 9.32YY23 pKa = 10.47IKK25 pKa = 10.76NSDD28 pKa = 3.48YY29 pKa = 11.64DD30 pKa = 3.56KK31 pKa = 10.92TKK33 pKa = 10.84ARR35 pKa = 11.84AIRR38 pKa = 11.84SLVSRR43 pKa = 11.84RR44 pKa = 11.84PRR46 pKa = 11.84RR47 pKa = 11.84SYY49 pKa = 10.38RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84SNRR56 pKa = 11.84RR57 pKa = 11.84GSRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84GYY64 pKa = 9.91RR65 pKa = 11.84GYY67 pKa = 10.61GAYY70 pKa = 9.85SRR72 pKa = 11.84RR73 pKa = 11.84GSASGSIGQRR83 pKa = 11.84LGGYY87 pKa = 9.47LGTMLGGAAQNLLKK101 pKa = 10.12TYY103 pKa = 9.06TGLGAYY109 pKa = 7.24QVKK112 pKa = 10.3GNALMPGAVISNPNPHH128 pKa = 6.69GGQVFRR134 pKa = 11.84GSDD137 pKa = 3.48YY138 pKa = 11.33LGDD141 pKa = 3.65IFSSPVAGQFTNQSFPINAALEE163 pKa = 4.05QTFPKK168 pKa = 10.31LAQFLQNFDD177 pKa = 3.39QYY179 pKa = 11.43VIEE182 pKa = 4.09GLIFEE187 pKa = 4.77FRR189 pKa = 11.84SMSCDD194 pKa = 3.11SLNSTNTALGSIIAACNYY212 pKa = 10.03NVLQAPFASKK222 pKa = 10.71AAMEE226 pKa = 4.3EE227 pKa = 4.46YY228 pKa = 10.45EE229 pKa = 4.59GGVSQRR235 pKa = 11.84PSSNMQFFVEE245 pKa = 4.64CARR248 pKa = 11.84AQSPMDD254 pKa = 3.39VLYY257 pKa = 10.0TRR259 pKa = 11.84TGAIPANADD268 pKa = 2.9LRR270 pKa = 11.84MYY272 pKa = 11.05DD273 pKa = 3.88LGNFQIASQGLQGTSVNMGEE293 pKa = 4.37LWVHH297 pKa = 5.07YY298 pKa = 9.65QVAGLKK304 pKa = 9.35EE305 pKa = 3.95KK306 pKa = 10.3IYY308 pKa = 11.07AGFGNYY314 pKa = 8.69NSAYY318 pKa = 9.4RR319 pKa = 11.84AVGSGGNNSNPLGNTRR335 pKa = 11.84TDD337 pKa = 3.38TYY339 pKa = 11.32NSGGFSLPTSTTIQFPNSPFVQTYY363 pKa = 10.7LMEE366 pKa = 4.38LVWTGGAVATLLPTFTATNAVFGQAFYY393 pKa = 10.74GPPSITTTAVQVITLVVQTTPNTVPVVTLSTTLVTLPTVPTVNIQITQQPNLITGVV449 pKa = 3.36

Molecular weight: 48.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

828

379

449

414.0

46.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.367 ± 0.684

0.966 ± 0.233

4.469 ± 1.231

5.556 ± 2.257

3.986 ± 0.367

7.729 ± 2.143

1.449 ± 0.786

5.314 ± 0.499

4.952 ± 2.133

7.005 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.019 ± 0.446

5.918 ± 0.598

5.797 ± 0.179

4.831 ± 0.751

5.918 ± 0.249

6.884 ± 0.887

6.763 ± 1.679

5.556 ± 0.881

1.57 ± 0.706

4.952 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski