Fusarium graminearum dsRNA mycovirus-4

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0TZ33|D0TZ33_9VIRU Uncharacterized protein OS=Fusarium graminearum dsRNA mycovirus-4 OX=687918 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84EE3 pKa = 3.87ILYY6 pKa = 10.46HH7 pKa = 4.73MWEE10 pKa = 4.26GDD12 pKa = 3.2MHH14 pKa = 6.6GLLRR18 pKa = 11.84LVTRR22 pKa = 11.84TLEE25 pKa = 4.3SEE27 pKa = 4.29AEE29 pKa = 3.97SDD31 pKa = 5.09NGDD34 pKa = 3.33EE35 pKa = 4.3EE36 pKa = 4.26QWIGSTMVPGYY47 pKa = 8.78PALPARR53 pKa = 11.84RR54 pKa = 11.84ADD56 pKa = 3.45YY57 pKa = 10.6GRR59 pKa = 11.84PFLDD63 pKa = 3.22VLRR66 pKa = 11.84WATNAAVAQGYY77 pKa = 7.63ATAQRR82 pKa = 11.84IVEE85 pKa = 4.06QDD87 pKa = 3.1PDD89 pKa = 3.17QDD91 pKa = 4.32RR92 pKa = 11.84EE93 pKa = 4.39LEE95 pKa = 4.16CLEE98 pKa = 4.29VVQDD102 pKa = 4.59AMVTGDD108 pKa = 4.11GRR110 pKa = 11.84FRR112 pKa = 11.84DD113 pKa = 3.29QWVFLL118 pKa = 4.1

Molecular weight: 13.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0TZ33|D0TZ33_9VIRU Uncharacterized protein OS=Fusarium graminearum dsRNA mycovirus-4 OX=687918 PE=4 SV=1
MM1 pKa = 7.74ADD3 pKa = 3.49MRR5 pKa = 11.84QMFAGATRR13 pKa = 11.84DD14 pKa = 3.42VSFNPIPGLEE24 pKa = 3.89NSYY27 pKa = 11.23LPIPAKK33 pKa = 9.94TDD35 pKa = 3.16SLTQLSKK42 pKa = 11.46KK43 pKa = 10.02MGDD46 pKa = 3.09LRR48 pKa = 11.84KK49 pKa = 9.83VVEE52 pKa = 4.7SGHH55 pKa = 5.78SLAVATGMRR64 pKa = 11.84GAAFDD69 pKa = 3.87TVNPEE74 pKa = 3.7EE75 pKa = 4.23KK76 pKa = 10.3AIAEE80 pKa = 4.04MGVAEE85 pKa = 4.34KK86 pKa = 10.39EE87 pKa = 4.16QYY89 pKa = 10.42LAWKK93 pKa = 9.98GGARR97 pKa = 11.84LSDD100 pKa = 4.19FDD102 pKa = 4.78PATCHH107 pKa = 6.33EE108 pKa = 4.84PTSKK112 pKa = 10.2WNGSIATLDD121 pKa = 2.88KK122 pKa = 10.26WLRR125 pKa = 11.84GLRR128 pKa = 11.84AMYY131 pKa = 10.51ADD133 pKa = 4.26EE134 pKa = 4.11EE135 pKa = 4.46LQRR138 pKa = 11.84EE139 pKa = 4.28HH140 pKa = 6.87AVWFAVRR147 pKa = 11.84APFMVPTITAHH158 pKa = 5.92VKK160 pKa = 10.18VLTAGKK166 pKa = 10.87SMVDD170 pKa = 2.86QHH172 pKa = 6.95AKK174 pKa = 10.17LRR176 pKa = 11.84PEE178 pKa = 4.12RR179 pKa = 11.84MAEE182 pKa = 3.85YY183 pKa = 10.29QSAKK187 pKa = 10.32RR188 pKa = 11.84ILADD192 pKa = 2.9AAYY195 pKa = 9.0RR196 pKa = 11.84VQVIKK201 pKa = 10.97RR202 pKa = 11.84EE203 pKa = 4.3INKK206 pKa = 8.24LTQEE210 pKa = 3.7IGRR213 pKa = 11.84AEE215 pKa = 4.28DD216 pKa = 3.51VVLTRR221 pKa = 11.84VRR223 pKa = 11.84HH224 pKa = 4.46YY225 pKa = 8.04QQKK228 pKa = 10.55VGTKK232 pKa = 6.52EE233 pKa = 3.95HH234 pKa = 7.02KK235 pKa = 8.99EE236 pKa = 3.78APEE239 pKa = 3.46NRR241 pKa = 11.84NRR243 pKa = 11.84KK244 pKa = 8.31RR245 pKa = 11.84RR246 pKa = 11.84GMPALGAPTDD256 pKa = 3.98LGGMTGTGRR265 pKa = 11.84EE266 pKa = 3.87ARR268 pKa = 11.84RR269 pKa = 11.84RR270 pKa = 11.84RR271 pKa = 11.84IDD273 pKa = 3.85LGPQLGTSPYY283 pKa = 10.93SLRR286 pKa = 11.84PWLGSDD292 pKa = 3.85HH293 pKa = 7.53PSTTHH298 pKa = 6.86LVPPLSPPSMRR309 pKa = 11.84RR310 pKa = 11.84IWAISSRR317 pKa = 11.84TPQCPPAGGSRR328 pKa = 11.84SRR330 pKa = 11.84PDD332 pKa = 3.12CRR334 pKa = 11.84HH335 pKa = 5.65WEE337 pKa = 4.02LL338 pKa = 4.61

Molecular weight: 37.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1168

118

712

389.3

44.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.503 ± 0.334

1.113 ± 0.137

7.277 ± 0.986

6.079 ± 0.915

3.596 ± 0.896

6.507 ± 0.558

2.911 ± 0.328

3.339 ± 0.204

4.11 ± 1.072

9.075 ± 0.546

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.62

2.397 ± 0.273

6.079 ± 0.589

4.366 ± 0.438

8.305 ± 0.748

5.736 ± 0.828

5.908 ± 0.215

5.479 ± 0.616

2.055 ± 0.37

3.253 ± 0.473
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski