Phycicoccus sp. CMS6Z-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Phycicoccus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A444BN28|A0A444BN28_9MICO SDR family NAD(P)-dependent oxidoreductase OS=Phycicoccus sp. CMS6Z-2 OX=2502783 GN=EPD83_09150 PE=3 SV=1
MM1 pKa = 7.19TRR3 pKa = 11.84RR4 pKa = 11.84SPRR7 pKa = 11.84LQTDD11 pKa = 3.6RR12 pKa = 11.84PTYY15 pKa = 9.47EE16 pKa = 3.92GRR18 pKa = 11.84VLPRR22 pKa = 11.84PDD24 pKa = 4.03EE25 pKa = 4.17EE26 pKa = 5.25VVDD29 pKa = 3.66QGLAFDD35 pKa = 5.66LGTLLDD41 pKa = 3.81RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84ALQVLGIGAAGGVLAACGTSSGGGATATASASATASATATAGAADD89 pKa = 3.98AADD92 pKa = 3.86TTLTEE97 pKa = 4.7IPDD100 pKa = 3.91EE101 pKa = 4.36TAGPYY106 pKa = 10.2PGDD109 pKa = 3.75GSNGADD115 pKa = 3.23VLEE118 pKa = 4.11QSGVVRR124 pKa = 11.84SDD126 pKa = 2.68ITASFGDD133 pKa = 4.19ASATAPGIPMTLTLTLLDD151 pKa = 3.74IAGGGAPMTGAAVYY165 pKa = 9.68VWHH168 pKa = 7.43CDD170 pKa = 3.2ALGQYY175 pKa = 10.92SMYY178 pKa = 9.62SQAVVGEE185 pKa = 4.06NYY187 pKa = 10.26LRR189 pKa = 11.84GVQVTDD195 pKa = 3.21DD196 pKa = 3.56TGAVTFTSVFPGCYY210 pKa = 8.86SGRR213 pKa = 11.84WPHH216 pKa = 5.27VHH218 pKa = 6.32FEE220 pKa = 4.47VYY222 pKa = 10.32PSVDD226 pKa = 4.55DD227 pKa = 3.74ITDD230 pKa = 3.44ASNAICTSQVALPKK244 pKa = 10.08GASDD248 pKa = 4.12AVYY251 pKa = 10.92ALDD254 pKa = 5.4DD255 pKa = 4.18YY256 pKa = 11.35PGSAQNLSSMSLTGDD271 pKa = 3.26NVFGDD276 pKa = 4.21DD277 pKa = 4.53GGVHH281 pKa = 5.78QLATVTGDD289 pKa = 3.16TSGYY293 pKa = 8.07TVALSVPIDD302 pKa = 3.56TATAPTAGGAPGGGQPPAPPSSS324 pKa = 3.34

Molecular weight: 32.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A444BHA2|A0A444BHA2_9MICO Histidine kinase OS=Phycicoccus sp. CMS6Z-2 OX=2502783 GN=EPD83_17105 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.93GRR40 pKa = 11.84AQLSAA45 pKa = 3.87

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4413

0

4413

1282195

24

2844

290.5

30.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.692 ± 0.052

0.673 ± 0.009

6.657 ± 0.034

5.531 ± 0.037

2.533 ± 0.024

9.732 ± 0.033

2.211 ± 0.022

2.506 ± 0.03

1.511 ± 0.03

10.227 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.675 ± 0.016

1.418 ± 0.019

6.023 ± 0.03

2.345 ± 0.023

8.402 ± 0.043

4.98 ± 0.029

6.2 ± 0.037

10.321 ± 0.037

1.555 ± 0.015

1.808 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski