Lactobacillus phage JNU_P5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3BE06|A0A6M3BE06_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P5 OX=2686384 PE=4 SV=1
MM1 pKa = 6.77TPAMIVTNLLIDD13 pKa = 4.39NLDD16 pKa = 3.81KK17 pKa = 11.46LPGLTEE23 pKa = 4.53GNICTYY29 pKa = 10.74SIDD32 pKa = 3.82NDD34 pKa = 3.81NVDD37 pKa = 4.2DD38 pKa = 4.17TNLIIVVGEE47 pKa = 4.01NAAGDD52 pKa = 3.68ASYY55 pKa = 11.58GNNDD59 pKa = 3.47VISTHH64 pKa = 6.31RR65 pKa = 11.84RR66 pKa = 11.84IQIQFYY72 pKa = 9.49YY73 pKa = 9.75PKK75 pKa = 10.41DD76 pKa = 3.81YY77 pKa = 11.21QEE79 pKa = 5.14DD80 pKa = 3.52MALIEE85 pKa = 4.28KK86 pKa = 8.4TVKK89 pKa = 10.57AFLRR93 pKa = 11.84AHH95 pKa = 7.68GYY97 pKa = 9.46RR98 pKa = 11.84CYY100 pKa = 11.22ADD102 pKa = 3.87AGHH105 pKa = 7.49IITPDD110 pKa = 2.88TMNILNTLKK119 pKa = 10.62FNYY122 pKa = 9.52QEE124 pKa = 5.54SEE126 pKa = 4.01DD127 pKa = 4.14TQNGG131 pKa = 3.37

Molecular weight: 14.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3BE80|A0A6M3BE80_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P5 OX=2686384 PE=4 SV=1
MM1 pKa = 7.54TSQLNGWLFILEE13 pKa = 4.37RR14 pKa = 11.84EE15 pKa = 4.37VSGITQRR22 pKa = 11.84LTQKK26 pKa = 9.01QRR28 pKa = 11.84RR29 pKa = 11.84FVDD32 pKa = 3.01EE33 pKa = 4.29HH34 pKa = 8.21VIFGNATQSYY44 pKa = 8.66KK45 pKa = 10.93AAGSTPARR53 pKa = 11.84QVGRR57 pKa = 11.84DD58 pKa = 3.64VVKK61 pKa = 10.73LRR63 pKa = 11.84VLHH66 pKa = 7.04RR67 pKa = 11.84ILKK70 pKa = 9.45VFATGLSGGFLYY82 pKa = 10.21TT83 pKa = 3.98

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

17752

30

1891

250.0

27.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.875 ± 0.65

0.513 ± 0.112

6.675 ± 0.346

5.453 ± 0.331

3.898 ± 0.235

6.343 ± 0.433

1.662 ± 0.18

6.309 ± 0.3

7.83 ± 0.419

7.729 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.226

6.185 ± 0.23

2.997 ± 0.221

4.771 ± 0.236

3.983 ± 0.297

7.357 ± 0.476

6.957 ± 0.463

5.836 ± 0.264

1.358 ± 0.105

3.763 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski