Pseudomonas phage tf

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Krylovvirus; Pseudomonas virus tf

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2FLQ6|I2FLQ6_9CAUD Uncharacterized protein OS=Pseudomonas phage tf OX=1114179 GN=tf_35 PE=4 SV=1
MM1 pKa = 7.71SKK3 pKa = 9.18FTLYY7 pKa = 10.84GYY9 pKa = 10.98LSDD12 pKa = 4.9NGDD15 pKa = 3.67GSASLHH21 pKa = 5.12WTSNPDD27 pKa = 4.02SVDD30 pKa = 3.82LDD32 pKa = 3.75DD33 pKa = 4.35EE34 pKa = 4.37QYY36 pKa = 11.69YY37 pKa = 8.79MCEE40 pKa = 4.04GDD42 pKa = 3.38WVEE45 pKa = 4.88VLTFDD50 pKa = 4.29SPEE53 pKa = 3.71AARR56 pKa = 11.84ACGLSWSDD64 pKa = 4.5EE65 pKa = 4.28EE66 pKa = 5.04GCC68 pKa = 5.11

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2FLM6|I2FLM6_9CAUD Uncharacterized protein OS=Pseudomonas phage tf OX=1114179 GN=tf_05 PE=4 SV=1
MM1 pKa = 7.05TPHH4 pKa = 5.74VRR6 pKa = 11.84PMEE9 pKa = 4.66TIMHH13 pKa = 6.24HH14 pKa = 5.06HH15 pKa = 7.16HH16 pKa = 6.69ARR18 pKa = 11.84ALRR21 pKa = 11.84IARR24 pKa = 11.84RR25 pKa = 11.84NYY27 pKa = 8.46LFCRR31 pKa = 11.84GVVARR36 pKa = 11.84RR37 pKa = 11.84IEE39 pKa = 3.93EE40 pKa = 3.94EE41 pKa = 3.56QRR43 pKa = 11.84IRR45 pKa = 11.84SMYY48 pKa = 9.9LANAHH53 pKa = 6.07NGAAVRR59 pKa = 11.84SYY61 pKa = 11.3YY62 pKa = 9.48FAKK65 pKa = 10.29VKK67 pKa = 10.57SAVRR71 pKa = 11.84SLEE74 pKa = 3.82LALVEE79 pKa = 4.51LQNQLSVLMTVEE91 pKa = 4.03RR92 pKa = 11.84LLEE95 pKa = 4.08KK96 pKa = 10.78AA97 pKa = 4.09

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14335

27

1021

199.1

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.083 ± 0.444

1.13 ± 0.128

5.72 ± 0.225

6.439 ± 0.423

3.558 ± 0.199

8.28 ± 0.417

2.023 ± 0.201

4.841 ± 0.195

5.72 ± 0.307

8.232 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.762 ± 0.175

4.402 ± 0.227

4.297 ± 0.241

4.311 ± 0.238

5.657 ± 0.251

5.755 ± 0.239

5.727 ± 0.272

7.018 ± 0.302

1.737 ± 0.126

3.307 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski