Mycetocola miduiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Mycetocola

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5CLU8|A0A1I5CLU8_9MICO Threonine dehydrogenase OS=Mycetocola miduiensis OX=995034 GN=SAMN05216219_2411 PE=3 SV=1
MM1 pKa = 7.34TNNVNNDD8 pKa = 3.62DD9 pKa = 3.87YY10 pKa = 11.95VEE12 pKa = 4.27PVLPADD18 pKa = 4.29EE19 pKa = 4.37YY20 pKa = 11.42DD21 pKa = 3.34GRR23 pKa = 11.84EE24 pKa = 4.08PVVSPLGADD33 pKa = 4.25DD34 pKa = 5.66DD35 pKa = 4.53DD36 pKa = 5.76HH37 pKa = 7.4NADD40 pKa = 3.54YY41 pKa = 7.47TTTGEE46 pKa = 4.68PIDD49 pKa = 4.27SPEE52 pKa = 4.01HH53 pKa = 5.67LAEE56 pKa = 4.24EE57 pKa = 4.61DD58 pKa = 3.73RR59 pKa = 11.84PHH61 pKa = 6.51ATTGNPLTGEE71 pKa = 4.47DD72 pKa = 4.13DD73 pKa = 4.05VEE75 pKa = 5.69AGADD79 pKa = 3.54YY80 pKa = 7.64TTPGNPITSVQHH92 pKa = 5.07EE93 pKa = 4.46VEE95 pKa = 4.19EE96 pKa = 4.88EE97 pKa = 4.04IPHH100 pKa = 5.82ATPGNNSAIPEE111 pKa = 4.09NN112 pKa = 3.87

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4Z5I5|A0A1I4Z5I5_9MICO SnoaL-like domain-containing protein OS=Mycetocola miduiensis OX=995034 GN=SAMN05216219_0728 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILGARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.99GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3145

0

3145

983503

31

1945

312.7

33.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.65 ± 0.05

0.505 ± 0.01

6.085 ± 0.036

5.686 ± 0.044

3.235 ± 0.025

8.721 ± 0.035

1.924 ± 0.019

4.842 ± 0.03

2.364 ± 0.032

10.266 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.84 ± 0.019

2.359 ± 0.021

5.264 ± 0.03

2.836 ± 0.025

6.784 ± 0.043

6.25 ± 0.031

6.168 ± 0.036

8.782 ± 0.039

1.413 ± 0.019

2.024 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski