Phenylobacterium zucineum (strain HLK1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Phenylobacterium; Phenylobacterium zucineum

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3838 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4RDL3|B4RDL3_PHEZH Integral membrane protein OS=Phenylobacterium zucineum (strain HLK1) OX=450851 GN=PHZ_c1992 PE=4 SV=1
MM1 pKa = 7.29SVGVSVPQSGDD12 pKa = 3.66PIVDD16 pKa = 3.4GLLFGSKK23 pKa = 8.33WSGTSLTFSFPASVADD39 pKa = 3.9LADD42 pKa = 3.61YY43 pKa = 7.34ATPLDD48 pKa = 3.59PTYY51 pKa = 10.88FGVLEE56 pKa = 4.32AAGQTAVRR64 pKa = 11.84AALARR69 pKa = 11.84WAAVAAVTFTEE80 pKa = 4.54VAPSAAADD88 pKa = 3.57LRR90 pKa = 11.84IYY92 pKa = 10.51HH93 pKa = 6.46YY94 pKa = 11.26DD95 pKa = 3.78DD96 pKa = 3.66GTLFTARR103 pKa = 11.84VVAFPDD109 pKa = 3.46ATPEE113 pKa = 4.31AGDD116 pKa = 3.75LQFGSMFVGPDD127 pKa = 3.28WSPGNYY133 pKa = 9.36HH134 pKa = 6.48YY135 pKa = 9.7FTVVHH140 pKa = 6.83EE141 pKa = 4.6IGHH144 pKa = 6.16ALGLKK149 pKa = 9.78HH150 pKa = 6.8PHH152 pKa = 6.38DD153 pKa = 4.42TPNGFPAADD162 pKa = 3.58SAVDD166 pKa = 3.93AVAEE170 pKa = 4.51TIMSYY175 pKa = 10.42RR176 pKa = 11.84SYY178 pKa = 11.33VGGPMGGYY186 pKa = 9.49SLQAGSYY193 pKa = 8.98PVGPMPSDD201 pKa = 3.11IAAIQHH207 pKa = 6.77LYY209 pKa = 9.01GPNWATNAGDD219 pKa = 3.44TTYY222 pKa = 10.8TFDD225 pKa = 4.01PSASVIFQAVWDD237 pKa = 4.14GGGTDD242 pKa = 3.63TYY244 pKa = 11.78NFASYY249 pKa = 10.59AADD252 pKa = 3.35LHH254 pKa = 6.5IDD256 pKa = 3.73LRR258 pKa = 11.84AGEE261 pKa = 4.07WSDD264 pKa = 5.33LGGQYY269 pKa = 11.15AVLDD273 pKa = 3.88TGDD276 pKa = 3.2ASIRR280 pKa = 11.84PSGNVANPHH289 pKa = 7.02LYY291 pKa = 10.38QGDD294 pKa = 3.91LRR296 pKa = 11.84SLIEE300 pKa = 3.94NAVGGSGDD308 pKa = 3.72DD309 pKa = 3.46TLTGNQAANTLTGGAGDD326 pKa = 4.37DD327 pKa = 4.06SLVGGDD333 pKa = 4.2GADD336 pKa = 3.46TLVGGSGNDD345 pKa = 3.66TLSGGAGSDD354 pKa = 3.15RR355 pKa = 11.84FTLGGGGDD363 pKa = 3.96VIADD367 pKa = 4.03FLAGDD372 pKa = 4.41RR373 pKa = 11.84IVAQGAVAGDD383 pKa = 3.6LTFDD387 pKa = 3.45LTGEE391 pKa = 4.28ILTIDD396 pKa = 3.91PDD398 pKa = 4.16GAGAAASFTVTVTGGLLSGYY418 pKa = 8.83EE419 pKa = 4.14LVAEE423 pKa = 4.42ADD425 pKa = 3.99GGLVWKK431 pKa = 10.08ALPPPPEE438 pKa = 4.61PPPPGPTHH446 pKa = 6.51TDD448 pKa = 3.16QPGEE452 pKa = 3.95GSDD455 pKa = 3.94FVVLSQEE462 pKa = 3.89GTTFAGAGADD472 pKa = 3.19WVLGSIEE479 pKa = 4.17GDD481 pKa = 2.97WVHH484 pKa = 6.7GNQGDD489 pKa = 4.26DD490 pKa = 3.5YY491 pKa = 10.89LHH493 pKa = 7.22GYY495 pKa = 8.43GAADD499 pKa = 3.36TVYY502 pKa = 10.56GGQGDD507 pKa = 3.85DD508 pKa = 3.98RR509 pKa = 11.84VFGDD513 pKa = 3.93EE514 pKa = 4.83GDD516 pKa = 3.73DD517 pKa = 4.76AIYY520 pKa = 11.06GDD522 pKa = 4.25MGSDD526 pKa = 3.24RR527 pKa = 11.84LDD529 pKa = 3.55GGTGDD534 pKa = 4.91DD535 pKa = 4.15VAHH538 pKa = 7.07GGPGDD543 pKa = 3.84DD544 pKa = 4.01VVEE547 pKa = 4.66GGQGADD553 pKa = 3.51LLYY556 pKa = 10.76GGKK559 pKa = 9.96DD560 pKa = 3.11ADD562 pKa = 4.34TVSGGAGDD570 pKa = 3.59DD571 pKa = 4.36WIWGDD576 pKa = 4.48LGDD579 pKa = 5.26DD580 pKa = 3.96RR581 pKa = 11.84LSGGAGADD589 pKa = 2.78RR590 pKa = 11.84FAFVAGYY597 pKa = 10.48AGNDD601 pKa = 3.44VIADD605 pKa = 4.28FSAADD610 pKa = 3.49GDD612 pKa = 4.24RR613 pKa = 11.84IEE615 pKa = 4.37LRR617 pKa = 11.84GVEE620 pKa = 4.69DD621 pKa = 4.85FAALTIGSSAAGATILLPTGEE642 pKa = 4.85VITLLGVPGASLGASDD658 pKa = 5.27FLFGG662 pKa = 5.0

Molecular weight: 66.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B4RDX2|PURA_PHEZH Adenylosuccinate synthetase OS=Phenylobacterium zucineum (strain HLK1) OX=450851 GN=purA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.11RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.53GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTKK25 pKa = 10.02NGQKK29 pKa = 9.43ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3838

0

3838

1282678

32

2838

334.2

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.46 ± 0.072

0.757 ± 0.011

5.577 ± 0.034

6.065 ± 0.039

3.481 ± 0.024

9.251 ± 0.039

1.813 ± 0.022

3.913 ± 0.027

2.727 ± 0.033

10.078 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.018

2.061 ± 0.027

5.852 ± 0.03

3.07 ± 0.02

8.168 ± 0.044

4.555 ± 0.027

4.794 ± 0.031

7.731 ± 0.036

1.433 ± 0.018

2.103 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski