Eubacterium nodatum ATCC 33099

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; [Eubacterium] nodatum

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W2BZ06|W2BZ06_9FIRM Preprotein translocase YajC subunit OS=Eubacterium nodatum ATCC 33099 OX=1161902 GN=yajC PE=3 SV=1
MM1 pKa = 7.48IGSSITVIGVLSGVIINGDD20 pKa = 3.46PAGVYY25 pKa = 9.58TIFGVSLTLLGVYY38 pKa = 10.39LSSKK42 pKa = 9.74EE43 pKa = 4.12IEE45 pKa = 4.28DD46 pKa = 3.84TAA48 pKa = 5.12

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W2BZL1|W2BZL1_9FIRM ABC transporter ATP-binding protein OS=Eubacterium nodatum ATCC 33099 OX=1161902 GN=HMPREF0378_1271 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 9.31QTYY5 pKa = 7.66QPKK8 pKa = 9.15KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.04GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATKK25 pKa = 10.07NGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.3RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.27KK41 pKa = 9.65LTAA44 pKa = 4.27

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1690

0

1690

541419

35

2906

320.4

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.614 ± 0.06

1.251 ± 0.022

5.689 ± 0.041

7.565 ± 0.066

4.259 ± 0.048

7.183 ± 0.056

1.575 ± 0.022

8.343 ± 0.059

8.986 ± 0.084

8.459 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.98 ± 0.03

4.974 ± 0.05

3.051 ± 0.033

2.434 ± 0.024

4.268 ± 0.048

6.096 ± 0.041

5.056 ± 0.046

6.58 ± 0.042

0.753 ± 0.018

3.883 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski