Halobacillus karajensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059NXV6|A0A059NXV6_9BACI Uncharacterized protein OS=Halobacillus karajensis OX=195088 GN=BN983_01854 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.31NLEE5 pKa = 4.13YY6 pKa = 10.74APNEE10 pKa = 3.63IFDD13 pKa = 3.87WYY15 pKa = 10.08MGCTQDD21 pKa = 4.2NYY23 pKa = 10.42IPSRR27 pKa = 11.84EE28 pKa = 4.08DD29 pKa = 3.17FYY31 pKa = 11.54EE32 pKa = 4.48AFDD35 pKa = 3.98GQHH38 pKa = 6.57AEE40 pKa = 3.71WADD43 pKa = 3.96RR44 pKa = 11.84LGLPDD49 pKa = 3.62VPEE52 pKa = 4.03VLEE55 pKa = 4.35PEE57 pKa = 4.0IDD59 pKa = 3.93DD60 pKa = 4.53EE61 pKa = 4.7VFSPQSTHH69 pKa = 6.85DD70 pKa = 3.27QMRR73 pKa = 11.84DD74 pKa = 3.4CGHH77 pKa = 6.89KK78 pKa = 10.77LSDD81 pKa = 4.1FEE83 pKa = 4.45

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059NZG0|A0A059NZG0_9BACI Uncharacterized protein OS=Halobacillus karajensis OX=195088 GN=BN983_01834 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 8.91VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3959

0

3959

1112030

29

1526

280.9

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.899 ± 0.038

0.653 ± 0.011

5.336 ± 0.03

8.001 ± 0.049

4.459 ± 0.037

7.201 ± 0.032

2.317 ± 0.022

7.217 ± 0.04

6.5 ± 0.036

9.476 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.004 ± 0.019

4.027 ± 0.023

3.754 ± 0.021

3.873 ± 0.026

4.261 ± 0.025

5.968 ± 0.03

5.377 ± 0.024

7.114 ± 0.033

1.096 ± 0.017

3.466 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski