Middle East respiratory syndrome-related coronavirus (MERS-CoV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Merbecovirus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R9UQM6|R9UQM6_MERS NS3D protein OS=Middle East respiratory syndrome-related coronavirus OX=1335626 GN=orf5 PE=4 SV=1
MM1 pKa = 6.86IHH3 pKa = 6.87SVFLLMFLLTPTEE16 pKa = 4.37SYY18 pKa = 11.55VDD20 pKa = 3.55VGPDD24 pKa = 3.43SVKK27 pKa = 10.47SACIEE32 pKa = 3.72VDD34 pKa = 2.94IQQTFFDD41 pKa = 4.38KK42 pKa = 10.29TWPRR46 pKa = 11.84PIDD49 pKa = 3.46VSKK52 pKa = 11.24ADD54 pKa = 4.31GIIYY58 pKa = 8.52PQGRR62 pKa = 11.84TYY64 pKa = 11.55SNITITYY71 pKa = 8.99QGLFPYY77 pKa = 10.39QGDD80 pKa = 3.55HH81 pKa = 6.45GDD83 pKa = 3.5MYY85 pKa = 11.3VYY87 pKa = 10.57SAGHH91 pKa = 5.77ATGTTPQKK99 pKa = 10.83LFVANYY105 pKa = 9.11SQDD108 pKa = 3.09VKK110 pKa = 11.08QFANGFVVRR119 pKa = 11.84IGAAANSTGTVIISPSTSATIRR141 pKa = 11.84KK142 pKa = 9.31IYY144 pKa = 8.72PAFMLGSSVGNFSDD158 pKa = 3.45GKK160 pKa = 8.72MGRR163 pKa = 11.84FFNHH167 pKa = 6.01TLVLLPDD174 pKa = 3.85GCGTLLRR181 pKa = 11.84AFYY184 pKa = 10.55CILEE188 pKa = 4.27PRR190 pKa = 11.84SGNHH194 pKa = 5.77CPAGNSYY201 pKa = 9.97TSFATYY207 pKa = 7.63HH208 pKa = 6.01TPATDD213 pKa = 4.12CSDD216 pKa = 3.09GNYY219 pKa = 9.81NRR221 pKa = 11.84NASLNSFKK229 pKa = 10.94EE230 pKa = 3.91YY231 pKa = 10.79FNLRR235 pKa = 11.84NCTFMYY241 pKa = 9.51TYY243 pKa = 10.63NITEE247 pKa = 4.15DD248 pKa = 4.93EE249 pKa = 3.99ILEE252 pKa = 4.35WFGITQTAQGVHH264 pKa = 6.39LFSSRR269 pKa = 11.84YY270 pKa = 7.82VDD272 pKa = 4.34LYY274 pKa = 9.95GGNMFQFATLPVYY287 pKa = 9.53DD288 pKa = 4.09TIKK291 pKa = 9.6YY292 pKa = 9.93YY293 pKa = 10.88SIIPHH298 pKa = 6.57SIRR301 pKa = 11.84SIQSDD306 pKa = 3.44RR307 pKa = 11.84KK308 pKa = 8.9AWAAFYY314 pKa = 10.51VYY316 pKa = 10.62KK317 pKa = 10.57LQPLTFLLDD326 pKa = 3.94FSVDD330 pKa = 3.3GYY332 pKa = 10.29IRR334 pKa = 11.84RR335 pKa = 11.84AIDD338 pKa = 3.32CGFNDD343 pKa = 5.2LSQLHH348 pKa = 6.59CSYY351 pKa = 11.2EE352 pKa = 4.41SFDD355 pKa = 3.95VEE357 pKa = 4.4SGVYY361 pKa = 9.77SVSSFEE367 pKa = 4.52AKK369 pKa = 10.07PSGSVVEE376 pKa = 3.97QAEE379 pKa = 4.32GVEE382 pKa = 4.81CDD384 pKa = 4.79FSPLLSGTPPQVYY397 pKa = 9.55NFKK400 pKa = 10.72RR401 pKa = 11.84LVFTNCNYY409 pKa = 10.82NLTKK413 pKa = 10.6LLSLFSVNDD422 pKa = 3.83FTCSQISPAAIASNCYY438 pKa = 10.04SSLILDD444 pKa = 3.82YY445 pKa = 10.84FSYY448 pKa = 9.64PLSMKK453 pKa = 10.31SDD455 pKa = 3.95LSVSSAGPISQFNYY469 pKa = 9.2KK470 pKa = 10.04QSFSNPTCLILATVPHH486 pKa = 6.9NLTTITKK493 pKa = 8.1PLKK496 pKa = 9.3YY497 pKa = 10.39SYY499 pKa = 10.58INKK502 pKa = 9.66CSRR505 pKa = 11.84LLSDD509 pKa = 5.19DD510 pKa = 3.58RR511 pKa = 11.84TEE513 pKa = 4.39VPQLVNANQYY523 pKa = 9.07SPCVSIVPSTVWEE536 pKa = 4.6DD537 pKa = 2.68GDD539 pKa = 4.03YY540 pKa = 10.91YY541 pKa = 10.98RR542 pKa = 11.84KK543 pKa = 9.48QLSPLEE549 pKa = 4.3GGGWLVASGSTVAMTEE565 pKa = 3.8QLQMGFGITVQYY577 pKa = 9.09GTDD580 pKa = 3.57TNSVCPKK587 pKa = 10.64LEE589 pKa = 3.84FANDD593 pKa = 3.52TKK595 pKa = 10.75IASQLGNCVEE605 pKa = 3.96YY606 pKa = 10.83SLYY609 pKa = 10.47GVSGRR614 pKa = 11.84GVFQNCTAVGVRR626 pKa = 11.84QQRR629 pKa = 11.84FVYY632 pKa = 10.14DD633 pKa = 4.07AYY635 pKa = 11.32QNLVGYY641 pKa = 10.55YY642 pKa = 9.85SDD644 pKa = 4.75DD645 pKa = 3.3GNYY648 pKa = 9.21YY649 pKa = 10.15CLRR652 pKa = 11.84ACVSVPVSVIYY663 pKa = 10.62DD664 pKa = 3.71KK665 pKa = 10.22EE666 pKa = 4.71TKK668 pKa = 8.48THH670 pKa = 5.81ATLFGSVACEE680 pKa = 4.33HH681 pKa = 6.93ISSTMSQYY689 pKa = 11.08SRR691 pKa = 11.84STRR694 pKa = 11.84SMLKK698 pKa = 10.21RR699 pKa = 11.84RR700 pKa = 11.84DD701 pKa = 3.4STYY704 pKa = 11.16GPLQTPVGCVLGLVNSSLFVEE725 pKa = 4.86DD726 pKa = 4.29CKK728 pKa = 11.38LPLGQSLCALPDD740 pKa = 3.78TPSTLTPRR748 pKa = 11.84SVRR751 pKa = 11.84SVPGEE756 pKa = 3.69MRR758 pKa = 11.84LASIAFNHH766 pKa = 7.19PIQVDD771 pKa = 3.76QLNSSYY777 pKa = 10.78FKK779 pKa = 11.13LSIPTNFSFGVTQEE793 pKa = 4.35YY794 pKa = 10.14IQTTIQKK801 pKa = 8.19VTVDD805 pKa = 3.83CKK807 pKa = 10.97QYY809 pKa = 10.71VCNGFQKK816 pKa = 10.67CEE818 pKa = 3.81QLLRR822 pKa = 11.84EE823 pKa = 4.09YY824 pKa = 11.27GQFCSKK830 pKa = 10.24INQALHH836 pKa = 5.92GANLRR841 pKa = 11.84QDD843 pKa = 3.46DD844 pKa = 4.08SVRR847 pKa = 11.84NLFASVKK854 pKa = 9.66SSQSSPIIPGFGGDD868 pKa = 3.83FNLTLLEE875 pKa = 4.3PVSISTGSRR884 pKa = 11.84SARR887 pKa = 11.84SAIEE891 pKa = 4.69DD892 pKa = 3.78LLFDD896 pKa = 4.46KK897 pKa = 10.28VTIADD902 pKa = 3.68PGYY905 pKa = 8.03MQGYY909 pKa = 9.01DD910 pKa = 3.52DD911 pKa = 6.26CMQQGPASARR921 pKa = 11.84DD922 pKa = 4.26LICAQYY928 pKa = 9.2VAGYY932 pKa = 9.81KK933 pKa = 9.75VLPPLMDD940 pKa = 3.55VNMEE944 pKa = 4.0AAYY947 pKa = 9.29TSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYY978 pKa = 10.26RR979 pKa = 11.84LNGVGITQQVLSEE992 pKa = 4.06NQKK995 pKa = 10.94LIANKK1000 pKa = 9.66FNQALGAMQTGFTTTNEE1017 pKa = 4.13AFRR1020 pKa = 11.84KK1021 pKa = 9.46VQDD1024 pKa = 3.57AVNNNAQALSKK1035 pKa = 10.55LASEE1039 pKa = 5.11LSNTFGAISASIGDD1053 pKa = 4.24IIQRR1057 pKa = 11.84LDD1059 pKa = 3.4VLEE1062 pKa = 4.26QDD1064 pKa = 3.65AQIDD1068 pKa = 3.67RR1069 pKa = 11.84LINGRR1074 pKa = 11.84LTTLNAFVAQQLVRR1088 pKa = 11.84SEE1090 pKa = 4.28SAALSAQLAKK1100 pKa = 10.93DD1101 pKa = 3.6KK1102 pKa = 11.5VNEE1105 pKa = 4.19CVKK1108 pKa = 10.57AQSKK1112 pKa = 10.61RR1113 pKa = 11.84SGFCGQGTHH1122 pKa = 5.83IVSFVVNAPNGLYY1135 pKa = 10.31FMHH1138 pKa = 6.54VGYY1141 pKa = 10.68YY1142 pKa = 8.92PSNHH1146 pKa = 6.15IEE1148 pKa = 4.11VVSAYY1153 pKa = 9.92GLCDD1157 pKa = 3.03AANPTNCIAPVNGYY1171 pKa = 9.08FIKK1174 pKa = 10.17TNNTRR1179 pKa = 11.84IVDD1182 pKa = 3.46EE1183 pKa = 4.15WSYY1186 pKa = 10.86TGSSFYY1192 pKa = 11.03APEE1195 pKa = 5.67PITSLNTKK1203 pKa = 9.23YY1204 pKa = 10.36VAPHH1208 pKa = 4.7VTYY1211 pKa = 11.0QNISTNLPPPLLGNSTGIDD1230 pKa = 3.71FQDD1233 pKa = 3.67EE1234 pKa = 3.86LDD1236 pKa = 3.71EE1237 pKa = 4.43FFKK1240 pKa = 11.2NVSTSIPNFGSLTQINTTLLDD1261 pKa = 3.48LTYY1264 pKa = 11.2EE1265 pKa = 4.16MLSLQQVVKK1274 pKa = 10.52ALNEE1278 pKa = 4.09SYY1280 pKa = 10.82IDD1282 pKa = 3.9LKK1284 pKa = 11.28EE1285 pKa = 4.19LGNYY1289 pKa = 7.68TYY1291 pKa = 11.04YY1292 pKa = 11.18NKK1294 pKa = 9.72WPWYY1298 pKa = 8.04IWLGFIAGLVALALCVFFILCCTGCGTNCMGKK1330 pKa = 9.91LKK1332 pKa = 10.64CNRR1335 pKa = 11.84CCDD1338 pKa = 3.3RR1339 pKa = 11.84YY1340 pKa = 10.78EE1341 pKa = 4.28EE1342 pKa = 4.54YY1343 pKa = 10.79DD1344 pKa = 3.78LEE1346 pKa = 4.13PHH1348 pKa = 6.39KK1349 pKa = 11.21VHH1351 pKa = 6.25VHH1353 pKa = 5.33

Molecular weight:
149.41 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|T2BBK0|T2BBK0_MERS Nucleoprotein OS=Middle East respiratory syndrome-related coronavirus OX=1335626 GN=N PE=3 SV=1
MM1 pKa = 7.86ASPAAPRR8 pKa = 11.84AVSFADD14 pKa = 3.78NNDD17 pKa = 3.02ITNTNLSRR25 pKa = 11.84GRR27 pKa = 11.84GRR29 pKa = 11.84NPKK32 pKa = 9.26PRR34 pKa = 11.84AAPNNTVSWYY44 pKa = 9.65TGLTQHH50 pKa = 6.38GKK52 pKa = 10.01VPLTFPPGQGVPLNANSTPAQNAGYY77 pKa = 9.33WRR79 pKa = 11.84RR80 pKa = 11.84QDD82 pKa = 3.96RR83 pKa = 11.84KK84 pKa = 9.7INTGNGIKK92 pKa = 9.96QLAPRR97 pKa = 11.84WYY99 pKa = 9.84FYY101 pKa = 10.45YY102 pKa = 10.42TGTGPEE108 pKa = 3.59AALPFRR114 pKa = 11.84AVKK117 pKa = 10.58DD118 pKa = 4.02GIVWVHH124 pKa = 5.14EE125 pKa = 4.51HH126 pKa = 6.58GATDD130 pKa = 3.5APSTFGTRR138 pKa = 11.84NPNNDD143 pKa = 3.31SAIVTQFAPGTKK155 pKa = 9.59LPKK158 pKa = 9.89NFHH161 pKa = 6.42IEE163 pKa = 4.18GTGGNSQSSSRR174 pKa = 11.84ASSVSRR180 pKa = 11.84NSSRR184 pKa = 11.84SSSQGSRR191 pKa = 11.84SGNSTRR197 pKa = 11.84GTSPGPSGIGAVGGDD212 pKa = 4.31LLYY215 pKa = 11.06LDD217 pKa = 5.07LLNRR221 pKa = 11.84LQALEE226 pKa = 4.11SGKK229 pKa = 10.41VKK231 pKa = 10.49QSQPKK236 pKa = 10.37VITKK240 pKa = 10.17KK241 pKa = 10.58DD242 pKa = 2.86AAAAKK247 pKa = 10.32NKK249 pKa = 8.51MRR251 pKa = 11.84HH252 pKa = 5.56KK253 pKa = 9.8RR254 pKa = 11.84TSTKK258 pKa = 10.27SFNMVQAFGLRR269 pKa = 11.84GPGDD273 pKa = 3.51LQGNFGDD280 pKa = 4.05LQLNKK285 pKa = 10.39LGTEE289 pKa = 4.19DD290 pKa = 4.25PRR292 pKa = 11.84WPQIAEE298 pKa = 4.14LAPTASAFMGMSQFKK313 pKa = 8.94LTHH316 pKa = 5.96QNNDD320 pKa = 2.91DD321 pKa = 4.12HH322 pKa = 6.97GNPVYY327 pKa = 10.43FLRR330 pKa = 11.84YY331 pKa = 9.33SGAIKK336 pKa = 10.61LDD338 pKa = 3.71PKK340 pKa = 10.57NPNYY344 pKa = 10.63NKK346 pKa = 9.76WLEE349 pKa = 3.97LLEE352 pKa = 4.17QNIDD356 pKa = 3.26AYY358 pKa = 9.71KK359 pKa = 10.18TFPKK363 pKa = 10.2KK364 pKa = 9.89EE365 pKa = 4.15KK366 pKa = 9.67KK367 pKa = 9.72QKK369 pKa = 10.33APKK372 pKa = 10.05EE373 pKa = 3.99EE374 pKa = 4.54STDD377 pKa = 3.68QMSEE381 pKa = 3.88PPKK384 pKa = 8.43EE385 pKa = 3.81QRR387 pKa = 11.84VQGSITQRR395 pKa = 11.84TRR397 pKa = 11.84TRR399 pKa = 11.84PSVQPGPMIDD409 pKa = 3.5VNTDD413 pKa = 2.58

Molecular weight:
45.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

14330

82

7078

1302.7

144.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.276

3.001 ± 0.308

5.304 ± 0.332

3.88 ± 0.195

5.045 ± 0.17

5.827 ± 0.241

2.066 ± 0.172

4.801 ± 0.183

5.255 ± 0.352

9.463 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.148

4.997 ± 0.161

4.215 ± 0.459

3.489 ± 0.301

3.643 ± 0.292

7.704 ± 0.511

6.936 ± 0.1

9.016 ± 0.587

1.137 ± 0.089

4.71 ± 0.242

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski