Microbispora sp. ATCC PTA-5024

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Microbispora; unclassified Microbispora

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W2EWE3|W2EWE3_9ACTN Uncharacterized protein OS=Microbispora sp. ATCC PTA-5024 OX=316330 GN=MPTA5024_15015 PE=4 SV=1
MM1 pKa = 8.78SMDD4 pKa = 3.6LHH6 pKa = 7.46DD7 pKa = 5.33GGHH10 pKa = 6.1TPADD14 pKa = 3.79DD15 pKa = 4.23PLADD19 pKa = 3.53VALDD23 pKa = 3.38ASGVHH28 pKa = 6.35LADD31 pKa = 5.79DD32 pKa = 4.97DD33 pKa = 4.44PPGPGVYY40 pKa = 10.45DD41 pKa = 3.95PDD43 pKa = 5.31GGTAGPYY50 pKa = 10.1GPEE53 pKa = 4.31GGLAGDD59 pKa = 4.46PAHH62 pKa = 7.04EE63 pKa = 3.76AAFWHH68 pKa = 6.02EE69 pKa = 3.98QAASDD74 pKa = 3.81TCAIAAQEE82 pKa = 4.08FVLDD86 pKa = 4.34DD87 pKa = 3.85LTGVPHH93 pKa = 6.72TEE95 pKa = 4.03AEE97 pKa = 4.21LTAVAEE103 pKa = 4.29EE104 pKa = 4.26NGWYY108 pKa = 10.08EE109 pKa = 4.29PGHH112 pKa = 5.49GTAMDD117 pKa = 3.92DD118 pKa = 3.29VGKK121 pKa = 10.54VLEE124 pKa = 4.66HH125 pKa = 6.37YY126 pKa = 9.01GVPVEE131 pKa = 4.45RR132 pKa = 11.84FGHH135 pKa = 5.23ATIADD140 pKa = 4.06LEE142 pKa = 4.4GALAGGRR149 pKa = 11.84DD150 pKa = 3.66VVAGVDD156 pKa = 3.54SGEE159 pKa = 3.68IWEE162 pKa = 4.47AQGLAQDD169 pKa = 4.01LDD171 pKa = 4.55GYY173 pKa = 10.15PGIPGQGADD182 pKa = 3.67HH183 pKa = 6.16VVQVTGVDD191 pKa = 3.41RR192 pKa = 11.84TDD194 pKa = 3.73PAHH197 pKa = 7.01PMVLMNDD204 pKa = 3.39SGSPSGAGAAVPLDD218 pKa = 3.65VFLDD222 pKa = 3.47AWGDD226 pKa = 3.52SGNFMIAAGGTRR238 pKa = 11.84GG239 pKa = 3.32

Molecular weight: 24.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W2ESD7|W2ESD7_9ACTN Uncharacterized protein OS=Microbispora sp. ATCC PTA-5024 OX=316330 GN=MPTA5024_18670 PE=4 SV=1
MM1 pKa = 7.09IKK3 pKa = 10.12KK4 pKa = 9.49RR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.21RR8 pKa = 11.84MAKK11 pKa = 9.41KK12 pKa = 9.99KK13 pKa = 9.77HH14 pKa = 5.81RR15 pKa = 11.84KK16 pKa = 8.55LLKK19 pKa = 8.44KK20 pKa = 9.17TRR22 pKa = 11.84IQRR25 pKa = 11.84RR26 pKa = 11.84NKK28 pKa = 9.73KK29 pKa = 9.84

Molecular weight: 3.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7799

0

7799

2428602

29

2240

311.4

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.708 ± 0.043

0.771 ± 0.008

5.913 ± 0.023

5.523 ± 0.029

2.795 ± 0.015

9.69 ± 0.032

2.139 ± 0.014

3.3 ± 0.018

1.757 ± 0.02

10.435 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.823 ± 0.011

1.705 ± 0.017

6.201 ± 0.027

2.489 ± 0.016

8.45 ± 0.036

4.936 ± 0.02

5.693 ± 0.026

8.968 ± 0.027

1.573 ± 0.012

2.13 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski