Anaeromassilibacillus sp. An172

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaeromassilibacillus; unclassified Anaeromassilibacillus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2508 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4N5S7|A0A1Y4N5S7_9FIRM Conjugal transfer protein OS=Anaeromassilibacillus sp. An172 OX=1965570 GN=B5F08_10600 PE=4 SV=1
MM1 pKa = 7.23FVLNDD6 pKa = 3.53YY7 pKa = 8.2MKK9 pKa = 10.91KK10 pKa = 9.57NLRR13 pKa = 11.84FIIPVAALIVLAIIFGIMYY32 pKa = 10.12IVNNNSDD39 pKa = 3.64QDD41 pKa = 3.89NTDD44 pKa = 2.84TTEE47 pKa = 4.16PVSTEE52 pKa = 3.71PQTTMTATEE61 pKa = 4.36EE62 pKa = 3.82LSQYY66 pKa = 10.6KK67 pKa = 9.9IGIMQFDD74 pKa = 4.23DD75 pKa = 3.62SQEE78 pKa = 3.98YY79 pKa = 10.69SDD81 pKa = 4.99VYY83 pKa = 11.53DD84 pKa = 3.86EE85 pKa = 5.23FVLAMEE91 pKa = 4.65HH92 pKa = 6.82RR93 pKa = 11.84GFTEE97 pKa = 4.19GVNVEE102 pKa = 4.23YY103 pKa = 11.05VYY105 pKa = 10.96MNAEE109 pKa = 4.18GDD111 pKa = 4.0SEE113 pKa = 4.46KK114 pKa = 10.86CSDD117 pKa = 3.54MAQSIVDD124 pKa = 3.92SGVDD128 pKa = 3.82LIFAISEE135 pKa = 4.16EE136 pKa = 4.29NAVAAYY142 pKa = 9.25NATKK146 pKa = 10.22DD147 pKa = 3.3IPIVFGAVNDD157 pKa = 4.13PEE159 pKa = 4.18EE160 pKa = 5.83AEE162 pKa = 5.39LIDD165 pKa = 3.85TNEE168 pKa = 3.8APGANVTGVSDD179 pKa = 3.86YY180 pKa = 11.27SPSIEE185 pKa = 4.56QIDD188 pKa = 4.38LIKK191 pKa = 10.15TVFPEE196 pKa = 4.1AKK198 pKa = 9.19TVGAVYY204 pKa = 10.8DD205 pKa = 4.08EE206 pKa = 4.98LNEE209 pKa = 4.18SSVTQISLAEE219 pKa = 4.14TQAQTNEE226 pKa = 4.02MTFDD230 pKa = 3.85KK231 pKa = 11.17YY232 pKa = 10.83PVNDD236 pKa = 3.56TTDD239 pKa = 3.7FDD241 pKa = 5.13SYY243 pKa = 9.4ITDD246 pKa = 3.79MIEE249 pKa = 4.07KK250 pKa = 9.88VDD252 pKa = 4.55VIYY255 pKa = 10.87LPYY258 pKa = 10.69DD259 pKa = 3.32EE260 pKa = 6.01LLIDD264 pKa = 4.96NIDD267 pKa = 4.16KK268 pKa = 9.18ITAVAYY274 pKa = 7.87EE275 pKa = 3.97HH276 pKa = 7.1GIPVVGGNEE285 pKa = 3.68EE286 pKa = 4.3MVQKK290 pKa = 10.75GCTIAYY296 pKa = 9.26GIDD299 pKa = 3.45YY300 pKa = 10.75ADD302 pKa = 3.73VGKK305 pKa = 10.55QSALMAVEE313 pKa = 4.65ILQNDD318 pKa = 4.23GTPAEE323 pKa = 4.23MPVRR327 pKa = 11.84YY328 pKa = 9.73LNEE331 pKa = 4.67LILYY335 pKa = 9.27VNTEE339 pKa = 3.95AKK341 pKa = 9.83EE342 pKa = 3.95KK343 pKa = 10.96LGFEE347 pKa = 5.02LDD349 pKa = 3.12EE350 pKa = 4.28TLQNKK355 pKa = 9.13AVLLPEE361 pKa = 4.35KK362 pKa = 10.7SEE364 pKa = 4.08EE365 pKa = 3.98

Molecular weight: 40.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4NDK2|A0A1Y4NDK2_9FIRM Pseudouridine synthase OS=Anaeromassilibacillus sp. An172 OX=1965570 GN=B5F08_05790 PE=4 SV=1
MM1 pKa = 7.9AEE3 pKa = 3.97RR4 pKa = 11.84PMNRR8 pKa = 11.84SRR10 pKa = 11.84KK11 pKa = 8.43PRR13 pKa = 11.84KK14 pKa = 8.99KK15 pKa = 10.03VCGFCVDD22 pKa = 3.21KK23 pKa = 11.52VEE25 pKa = 4.75NIDD28 pKa = 3.57YY29 pKa = 10.94KK30 pKa = 11.15DD31 pKa = 3.08IARR34 pKa = 11.84LRR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 8.42MSEE41 pKa = 3.7RR42 pKa = 11.84GKK44 pKa = 10.14ILPRR48 pKa = 11.84RR49 pKa = 11.84VTGTCAAHH57 pKa = 5.98QRR59 pKa = 11.84ALTVAIKK66 pKa = 10.31RR67 pKa = 11.84ARR69 pKa = 11.84HH70 pKa = 5.37LALLPYY76 pKa = 8.42TTDD79 pKa = 2.85

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2508

0

2508

812747

34

4072

324.1

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.074 ± 0.051

1.588 ± 0.025

5.872 ± 0.046

7.493 ± 0.05

4.359 ± 0.038

6.966 ± 0.046

1.471 ± 0.018

7.59 ± 0.044

7.288 ± 0.05

8.414 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.025

5.087 ± 0.043

3.246 ± 0.028

2.856 ± 0.026

3.895 ± 0.041

6.426 ± 0.043

5.745 ± 0.05

6.858 ± 0.039

0.841 ± 0.018

4.137 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski