Oceanispirochaeta sp. M1

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Oceanispirochaeta; unclassified Oceanispirochaeta

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370ARX4|A0A370ARX4_9SPIO LacI family transcriptional regulator OS=Oceanispirochaeta sp. M1 OX=2283433 GN=DV872_09130 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.34KK3 pKa = 9.12TIVLLTIVLALVAAPVFAEE22 pKa = 4.17TGFTGSVEE30 pKa = 4.37FKK32 pKa = 10.73YY33 pKa = 9.83GTDD36 pKa = 3.66FSDD39 pKa = 4.18FATDD43 pKa = 3.2TGNDD47 pKa = 3.49TKK49 pKa = 10.85ISLDD53 pKa = 3.47GKK55 pKa = 9.89VGEE58 pKa = 4.76FSTISVGIEE67 pKa = 3.36ADD69 pKa = 3.69EE70 pKa = 4.43NSNASATLMTLSQDD84 pKa = 3.22VTGALGVEE92 pKa = 4.8GPVSFAYY99 pKa = 9.78KK100 pKa = 9.48MGKK103 pKa = 7.41QTYY106 pKa = 9.2QPADD110 pKa = 3.36YY111 pKa = 10.21SGVGGYY117 pKa = 10.31EE118 pKa = 3.83DD119 pKa = 4.15AGRR122 pKa = 11.84DD123 pKa = 3.48AKK125 pKa = 10.56IGQGYY130 pKa = 8.36EE131 pKa = 3.92ADD133 pKa = 3.86DD134 pKa = 3.83TDD136 pKa = 3.82FTTNSMLGTVLTFGFADD153 pKa = 4.42MIMIDD158 pKa = 3.53AVVFPASYY166 pKa = 10.39FEE168 pKa = 4.36KK169 pKa = 10.83AADD172 pKa = 3.51AANGYY177 pKa = 10.17FDD179 pKa = 5.42ADD181 pKa = 3.51NFAEE185 pKa = 4.48FAVNAYY191 pKa = 7.88GTFGPAQVSVYY202 pKa = 8.65YY203 pKa = 10.61TMSNLSYY210 pKa = 10.72LADD213 pKa = 3.8SDD215 pKa = 4.22GDD217 pKa = 4.52AIDD220 pKa = 4.2EE221 pKa = 4.54AGDD224 pKa = 3.73MIGANLGLVIDD235 pKa = 4.39ALSVGVLWEE244 pKa = 4.04TDD246 pKa = 3.45LEE248 pKa = 4.43KK249 pKa = 11.04DD250 pKa = 3.55FQAATGVTGTKK261 pKa = 10.05PMNTEE266 pKa = 3.04IGIAAKK272 pKa = 10.26YY273 pKa = 10.13VIGSVTPGIAFKK285 pKa = 9.79TAFYY289 pKa = 10.65DD290 pKa = 4.15GSDD293 pKa = 3.67FAADD297 pKa = 3.36SGLGLNVTWAAAEE310 pKa = 4.14MFNVTAAVKK319 pKa = 10.55NSFVFDD325 pKa = 4.32DD326 pKa = 4.69LGDD329 pKa = 3.69TLGYY333 pKa = 10.07EE334 pKa = 4.11VGVDD338 pKa = 3.49YY339 pKa = 10.8TLDD342 pKa = 3.39GVKK345 pKa = 10.63YY346 pKa = 10.59SIGYY350 pKa = 8.01TDD352 pKa = 3.53GSEE355 pKa = 4.34FKK357 pKa = 10.69AYY359 pKa = 10.07DD360 pKa = 3.78AEE362 pKa = 5.12LSDD365 pKa = 3.84TGNMYY370 pKa = 10.84VKK372 pKa = 10.57VGASFF377 pKa = 3.67

Molecular weight: 39.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370AUF5|A0A370AUF5_9SPIO HAMP domain-containing protein OS=Oceanispirochaeta sp. M1 OX=2283433 GN=DV872_12745 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.23KK4 pKa = 10.87SMIVKK9 pKa = 10.12CNRR12 pKa = 11.84KK13 pKa = 8.44QKK15 pKa = 10.53YY16 pKa = 6.66STRR19 pKa = 11.84EE20 pKa = 3.65YY21 pKa = 10.59NRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PRR31 pKa = 11.84GYY33 pKa = 8.93MRR35 pKa = 11.84KK36 pKa = 9.48FEE38 pKa = 3.99MCRR41 pKa = 11.84ICFRR45 pKa = 11.84KK46 pKa = 9.57LANEE50 pKa = 3.91GLIPGVTKK58 pKa = 10.8SSWW61 pKa = 2.87

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5141

0

5141

1776840

27

2815

345.6

38.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.584 ± 0.038

1.011 ± 0.011

5.812 ± 0.027

7.163 ± 0.039

4.687 ± 0.023

6.978 ± 0.033

1.782 ± 0.014

7.804 ± 0.036

6.122 ± 0.03

10.358 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.883 ± 0.018

4.394 ± 0.025

3.829 ± 0.019

3.039 ± 0.017

4.636 ± 0.025

7.43 ± 0.028

4.861 ± 0.021

5.915 ± 0.027

1.134 ± 0.012

3.577 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski