Alistipes sp. AF17-16

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; unclassified Alistipes

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396MGY2|A0A396MGY2_9BACT Uncharacterized protein OS=Alistipes sp. AF17-16 OX=2292190 GN=DWW79_01185 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.11KK3 pKa = 10.37YY4 pKa = 9.99KK5 pKa = 10.41CQVCGYY11 pKa = 9.47IYY13 pKa = 10.46DD14 pKa = 4.37PEE16 pKa = 5.18VGDD19 pKa = 4.21PDD21 pKa = 4.17NGIEE25 pKa = 4.31PGTAFEE31 pKa = 5.37DD32 pKa = 5.05LPDD35 pKa = 3.97DD36 pKa = 4.27WVCPLCQEE44 pKa = 4.1GKK46 pKa = 10.38EE47 pKa = 4.16VFEE50 pKa = 4.66ALL52 pKa = 4.07

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396M7A7|A0A396M7A7_9BACT Ribosome maturation factor RimM OS=Alistipes sp. AF17-16 OX=2292190 GN=rimM PE=3 SV=1
MM1 pKa = 6.99EE2 pKa = 4.63QNSFRR7 pKa = 11.84IRR9 pKa = 11.84LLRR12 pKa = 11.84AVSEE16 pKa = 4.23AMRR19 pKa = 11.84PAWRR23 pKa = 11.84TAVWVIKK30 pKa = 9.52MVVPITLAISILEE43 pKa = 3.9HH44 pKa = 6.84LGVIGRR50 pKa = 11.84ISEE53 pKa = 4.48WIAPLFGLVGLPGDD67 pKa = 3.82SGLVFLTAALSNNYY81 pKa = 9.75AAVAVIATLGFDD93 pKa = 3.52YY94 pKa = 10.74RR95 pKa = 11.84SVVILAVMALVCHH108 pKa = 6.19CLIIEE113 pKa = 4.43SAIQHH118 pKa = 5.32KK119 pKa = 9.24TGASGLKK126 pKa = 7.95MTLLRR131 pKa = 11.84IGMAFIAAALMNLILPKK148 pKa = 10.72HH149 pKa = 4.21MTGRR153 pKa = 11.84LFLPPAGVSPEE164 pKa = 3.78TWAEE168 pKa = 4.53AGLLWLKK175 pKa = 10.03TILPLSLKK183 pKa = 9.97IGIIIVSLNILQRR196 pKa = 11.84ILQEE200 pKa = 4.2FRR202 pKa = 11.84LLDD205 pKa = 4.28LLTVPLQPLMAVFGLPRR222 pKa = 11.84STSFLWIISNVVGLTYY238 pKa = 10.81GGAALVDD245 pKa = 4.84EE246 pKa = 4.97IRR248 pKa = 11.84RR249 pKa = 11.84GAISRR254 pKa = 11.84EE255 pKa = 3.85DD256 pKa = 3.45SRR258 pKa = 11.84LLNAHH263 pKa = 5.58VAISHH268 pKa = 6.36SLLEE272 pKa = 4.33DD273 pKa = 3.12TAIFYY278 pKa = 10.81SIGVGLFWLLVPRR291 pKa = 11.84IILAICTVWIIRR303 pKa = 11.84LFVRR307 pKa = 11.84LRR309 pKa = 11.84NPRR312 pKa = 11.84RR313 pKa = 11.84SIAPGG318 pKa = 3.12

Molecular weight: 34.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2605

0

2605

907528

23

2235

348.4

38.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.318 ± 0.05

1.235 ± 0.017

5.801 ± 0.032

6.221 ± 0.04

4.487 ± 0.031

7.507 ± 0.042

1.731 ± 0.019

6.345 ± 0.046

5.431 ± 0.043

8.99 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.019

4.186 ± 0.032

4.237 ± 0.029

3.178 ± 0.027

6.163 ± 0.045

5.843 ± 0.031

5.531 ± 0.035

7.007 ± 0.037

1.223 ± 0.021

4.03 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski