Orf virus (strain Goat/Texas/SA00/2000) (OV-SA00) (Orf virus-San Angelo 2000)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Parapoxvirus; Orf virus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6TVP1|Q6TVP1_ORFSA Major core protein 4b OS=Orf virus (strain Goat/Texas/SA00/2000) OX=647330 PE=3 SV=1
MM1 pKa = 7.37EE2 pKa = 4.76EE3 pKa = 4.16SVAVEE8 pKa = 3.98YY9 pKa = 11.02ADD11 pKa = 3.81EE12 pKa = 5.79DD13 pKa = 3.71EE14 pKa = 5.97DD15 pKa = 4.6EE16 pKa = 5.02IEE18 pKa = 4.13EE19 pKa = 4.35EE20 pKa = 4.33YY21 pKa = 11.11EE22 pKa = 4.32EE23 pKa = 4.32EE24 pKa = 5.49DD25 pKa = 3.33EE26 pKa = 5.62DD27 pKa = 4.63EE28 pKa = 4.6EE29 pKa = 4.59EE30 pKa = 4.35EE31 pKa = 4.42SAEE34 pKa = 4.07GAADD38 pKa = 3.62SSVSDD43 pKa = 3.77VALSAAEE50 pKa = 4.02KK51 pKa = 10.32LVASEE56 pKa = 4.66VPDD59 pKa = 4.1DD60 pKa = 4.48AAAADD65 pKa = 3.83TNVRR69 pKa = 11.84QRR71 pKa = 11.84VTARR75 pKa = 11.84VEE77 pKa = 3.97EE78 pKa = 4.33LKK80 pKa = 10.9ARR82 pKa = 11.84YY83 pKa = 6.43TRR85 pKa = 11.84RR86 pKa = 11.84MSLFEE91 pKa = 3.78LTGIVAEE98 pKa = 4.73SFNLLCRR105 pKa = 11.84GRR107 pKa = 11.84LPLVADD113 pKa = 4.18AADD116 pKa = 3.69PALDD120 pKa = 3.83NEE122 pKa = 4.61LKK124 pKa = 10.61VVVRR128 pKa = 11.84EE129 pKa = 4.22LEE131 pKa = 4.08EE132 pKa = 4.58GVCPIVIEE140 pKa = 4.32KK141 pKa = 10.61NGEE144 pKa = 3.89FLSPGDD150 pKa = 4.39FDD152 pKa = 4.67PEE154 pKa = 4.04CLRR157 pKa = 11.84YY158 pKa = 9.61HH159 pKa = 6.41LTYY162 pKa = 8.24MTDD165 pKa = 2.63LWKK168 pKa = 11.0SQGRR172 pKa = 11.84MM173 pKa = 3.26

Molecular weight: 19.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6TVN4|Q6TVN4_ORFSA Major core protein 4a precursor OS=Orf virus (strain Goat/Texas/SA00/2000) OX=647330 PE=3 SV=1
MM1 pKa = 7.63ASEE4 pKa = 4.98KK5 pKa = 9.75MAQRR9 pKa = 11.84PQSSYY14 pKa = 11.53DD15 pKa = 3.53DD16 pKa = 3.81YY17 pKa = 12.08VNTLQKK23 pKa = 10.13IAPHH27 pKa = 5.86LRR29 pKa = 11.84SLLTQLNNEE38 pKa = 3.88QVIRR42 pKa = 11.84GGATSGDD49 pKa = 3.68VDD51 pKa = 4.08CVGGAGALEE60 pKa = 4.54ASSSSTRR67 pKa = 11.84RR68 pKa = 11.84GSSSSSSRR76 pKa = 11.84KK77 pKa = 8.14RR78 pKa = 11.84TTGTRR83 pKa = 11.84STGSRR88 pKa = 11.84STGSRR93 pKa = 11.84SSGTRR98 pKa = 11.84SSSSTRR104 pKa = 11.84SRR106 pKa = 11.84RR107 pKa = 11.84SGAPSRR113 pKa = 11.84RR114 pKa = 11.84NNDD117 pKa = 2.69GVYY120 pKa = 10.6SGMNACDD127 pKa = 4.55DD128 pKa = 3.9EE129 pKa = 4.69TTIQAVSNCGKK140 pKa = 9.93IVYY143 pKa = 9.81GVVKK147 pKa = 10.54NGKK150 pKa = 9.45LDD152 pKa = 3.46VQGTVGEE159 pKa = 4.35VCEE162 pKa = 4.78DD163 pKa = 3.54LLGIDD168 pKa = 4.0SVNGGRR174 pKa = 11.84KK175 pKa = 9.2KK176 pKa = 10.72SSSGKK181 pKa = 10.21SGTGTSRR188 pKa = 11.84RR189 pKa = 11.84GGTGTGTGRR198 pKa = 11.84SSTSGTTGRR207 pKa = 11.84RR208 pKa = 11.84STNSGTSSSASSSRR222 pKa = 11.84RR223 pKa = 11.84RR224 pKa = 11.84ASSSNTPCSTTSSASTGARR243 pKa = 11.84RR244 pKa = 11.84RR245 pKa = 11.84ASNLMNAMDD254 pKa = 5.46LDD256 pKa = 4.16SGMCC260 pKa = 3.85

Molecular weight: 26.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

42036

53

1289

325.9

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.379 ± 0.315

2.391 ± 0.119

5.916 ± 0.117

5.885 ± 0.128

4.527 ± 0.164

5.486 ± 0.15

2.177 ± 0.096

3.778 ± 0.159

3.59 ± 0.164

9.585 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.107

3.454 ± 0.144

4.989 ± 0.224

2.129 ± 0.082

8.2 ± 0.212

6.98 ± 0.215

5.374 ± 0.155

8.757 ± 0.172

0.692 ± 0.057

2.883 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski