Shimia thalassica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Shimia

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1I5X0|A0A0P1I5X0_9RHOB Probable multidrug resistance protein NorM OS=Shimia thalassica OX=1715693 GN=mepA_2 PE=4 SV=1
MM1 pKa = 6.96MAFSIRR7 pKa = 11.84VFDD10 pKa = 4.25MIYY13 pKa = 10.73EE14 pKa = 4.45VIGTAADD21 pKa = 3.95DD22 pKa = 5.48LIVAPPNPNNQAVIYY37 pKa = 9.88GRR39 pKa = 11.84EE40 pKa = 4.0GDD42 pKa = 4.39DD43 pKa = 3.55VLAAHH48 pKa = 6.94SNNANNNDD56 pKa = 3.81TLRR59 pKa = 11.84GDD61 pKa = 3.69SGNDD65 pKa = 2.98TLYY68 pKa = 10.91AGNGGDD74 pKa = 3.89VLFGGPDD81 pKa = 3.76DD82 pKa = 5.59DD83 pKa = 6.25LLFGPSGHH91 pKa = 6.14GHH93 pKa = 5.68ATLNGGTGDD102 pKa = 3.98DD103 pKa = 4.39TISSASLTRR112 pKa = 11.84SYY114 pKa = 10.49LGPMQVHH121 pKa = 6.63GGEE124 pKa = 4.33GDD126 pKa = 3.87DD127 pKa = 4.54VIHH130 pKa = 7.09LGGGNHH136 pKa = 7.67WIYY139 pKa = 11.16GDD141 pKa = 3.85NGNDD145 pKa = 4.49LIVGSHH151 pKa = 6.51KK152 pKa = 10.43FFNWEE157 pKa = 4.05HH158 pKa = 6.36LFGGAGNDD166 pKa = 4.03TILGQQGEE174 pKa = 4.45DD175 pKa = 3.76TISGGSGDD183 pKa = 3.8DD184 pKa = 3.81ALWGGNLSDD193 pKa = 5.17HH194 pKa = 7.04IYY196 pKa = 11.01GGTGDD201 pKa = 4.54DD202 pKa = 3.64RR203 pKa = 11.84LFGGQYY209 pKa = 11.09GDD211 pKa = 3.8TLQGGDD217 pKa = 3.81GFDD220 pKa = 5.51FIDD223 pKa = 4.48GGNGVDD229 pKa = 4.73DD230 pKa = 4.19LWGGADD236 pKa = 3.27EE237 pKa = 4.76DD238 pKa = 3.72RR239 pKa = 11.84FYY241 pKa = 11.51RR242 pKa = 11.84EE243 pKa = 3.52ASDD246 pKa = 3.71YY247 pKa = 11.29LFDD250 pKa = 6.48RR251 pKa = 11.84IHH253 pKa = 7.58DD254 pKa = 3.99YY255 pKa = 11.01DD256 pKa = 4.17ASEE259 pKa = 4.31GDD261 pKa = 3.62LLHH264 pKa = 7.12FGGEE268 pKa = 4.31AYY270 pKa = 10.23NANQFHH276 pKa = 6.49VSFHH280 pKa = 5.29NTNNGEE286 pKa = 4.14AGIQDD291 pKa = 4.89AIITYY296 pKa = 10.57APTNQVLWILTDD308 pKa = 3.44VEE310 pKa = 4.27HH311 pKa = 7.92AEE313 pKa = 4.88DD314 pKa = 3.3INIRR318 pKa = 11.84LFTGATIDD326 pKa = 4.94LII328 pKa = 4.35

Molecular weight: 34.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1IJD5|A0A0P1IJD5_9RHOB 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Shimia thalassica OX=1715693 GN=ispE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4063

0

4063

1322757

29

8219

325.6

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.001 ± 0.05

0.887 ± 0.015

6.446 ± 0.065

6.031 ± 0.04

3.995 ± 0.028

8.716 ± 0.08

2.094 ± 0.023

5.309 ± 0.031

3.651 ± 0.04

9.737 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.839 ± 0.027

3.077 ± 0.029

4.678 ± 0.037

3.307 ± 0.024

5.786 ± 0.046

5.696 ± 0.037

5.686 ± 0.044

7.366 ± 0.034

1.374 ± 0.017

2.323 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski