Aurantimonas phage AmM-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8IL15|A0A0A8IL15_9CAUD Uncharacterized protein OS=Aurantimonas phage AmM-1 OX=1503929 PE=4 SV=1
MM1 pKa = 7.25QEE3 pKa = 4.93AIMTPMTEE11 pKa = 4.23PPRR14 pKa = 11.84DD15 pKa = 3.41ATRR18 pKa = 11.84IPRR21 pKa = 11.84PLDD24 pKa = 3.39DD25 pKa = 3.23WHH27 pKa = 7.1EE28 pKa = 4.56DD29 pKa = 3.64DD30 pKa = 6.55GDD32 pKa = 4.11VLWWCWRR39 pKa = 11.84DD40 pKa = 3.87GEE42 pKa = 4.46WLGEE46 pKa = 3.94APYY49 pKa = 10.76VGSPLDD55 pKa = 3.36LGYY58 pKa = 8.83TVEE61 pKa = 4.4CHH63 pKa = 4.6THH65 pKa = 6.04AEE67 pKa = 4.11RR68 pKa = 11.84GEE70 pKa = 4.02APAARR75 pKa = 11.84FNVGGWPGYY84 pKa = 7.3HH85 pKa = 5.43THH87 pKa = 5.81WTPLPSQPYY96 pKa = 10.43APGEE100 pKa = 4.42SNN102 pKa = 3.47

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8IKA5|A0A0A8IKA5_9CAUD Uncharacterized protein OS=Aurantimonas phage AmM-1 OX=1503929 PE=4 SV=1
MM1 pKa = 7.35LRR3 pKa = 11.84VRR5 pKa = 11.84PADD8 pKa = 3.72LLDD11 pKa = 3.58AAKK14 pKa = 10.4RR15 pKa = 11.84EE16 pKa = 4.18RR17 pKa = 11.84EE18 pKa = 3.8RR19 pKa = 11.84RR20 pKa = 11.84TIALEE25 pKa = 3.57AARR28 pKa = 11.84AAEE31 pKa = 4.17HH32 pKa = 6.66RR33 pKa = 11.84SIAAGGAPGATYY45 pKa = 8.85WHH47 pKa = 6.56ARR49 pKa = 11.84HH50 pKa = 6.13RR51 pKa = 11.84AEE53 pKa = 4.0QTLQAAHH60 pKa = 7.11RR61 pKa = 11.84LEE63 pKa = 4.21AAIAEE68 pKa = 4.3AMAGG72 pKa = 3.51

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14802

58

760

220.9

23.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.397 ± 0.478

0.885 ± 0.107

6.492 ± 0.183

6.269 ± 0.229

3.283 ± 0.171

8.675 ± 0.215

1.811 ± 0.138

4.432 ± 0.223

3.06 ± 0.177

8.08 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.113

2.067 ± 0.17

5.607 ± 0.255

3.02 ± 0.139

8.458 ± 0.299

5.182 ± 0.185

5.297 ± 0.294

6.877 ± 0.303

1.662 ± 0.16

1.973 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski